Through bioinformatic methods screening out the key miRNAs and mRNAs differentially expressed in mouse testicular tissues with their increasing age, to provide new directions for the research of spermatogenesis. Methods: Microarray data on miRNAs and mRNAs of spermatogenesis were searched in NCBI's GEO database, and the datasets were identified as GSE41857 and GSE41858. GEO2R online analysis of the dataset GSE41857 was used to derive the differentially expression of miRNAs related to spermatogenesis, and the Lianchuan Omicstudio was used to predict their target genes. GEO2R online analysis of the dataset GSE41858 was used to derive the differentially expression of mRNAs associated with spermatogenesis. The target genes as mentioned above were intersected with the target genes of the miRNA to obtain the final differentially expressed genes. GO enrichment and KEGG pathway analysis of the differentially expressed genes were performed by DAVID, and STRING was applied to construct a protein interaction network associated with differentially expressed genes. Results: Analysis of the dataset GSE41857 yielded 52 differentially expressed miRNAs with 17,224 target genes. Analysis of the dataset GSE41858 yielded 619 differentially expressed genes, and finally 485 differentially expressed genes of intersection were obtained. GO enrichment analysis of biological processes (BP), mainly included spermatogenesis, cell differentiation, and cilia assembly, etc. Cell composition (CC) mainly included cytoplasm, nucleus, and cytoskeleton, etc. Molecular function (MF) mainly included protein binding, RNA polymerase II sequence-specific DNA binding transcription factor binding, and integrin binding, etc. The pathways obtained by KEGG analysis of differentially expressed genes mainly included axon guidance, hepatitis B, and Hippo signaling pathway, etc. The top 20 hub genes analyzed by cytoscape were Tgfb1/1, Tns3, Trn1, Csk, Zyx, Capn5, Capn1, Crebbp, Plxna1, Plxna3, Plxna4, Plxnb1, Itga9, Lama5, Fras1, Pdlim3, Ccnb2, E2f1, Ncor2, and Inppl1. Conclusion: The miRNA-mRNA regulatory network in mouse spermatogenesis has been constructed in this study, and some miRNAs and novel genes are uncovered, which may be involved in spermatogenesis. The new directions are provided for further elucidation of spermatogenesis.

2022 Vol. 30　(9):　1944- [Abstract]（ 801 ） HTML (0 KB)  PDF  (0 KB)  ( 25 )

To study the problems existing in the performance appraisal of maternal and child health care institutions in Xinjiang, and put forward suggestions and measures for improvement. Methods: Based on the national performance appraisal information system of maternal and child health care institutions, the data were collected. The unified evaluation and scoring were conducted by “performance appraisal standards of maternal and child health care institutions” 56 performance evaluation indicators of 106 maternal and child health care institutions in Xinjiang were evaluated and scored one by one, and the scores differences of these indicators were compared among the institutions. Results: The average scores of the tertiary institutions (4 institutions), the secondary institutions (33 institutions), and the primary institutions (41 institutions) were 451.4±51.7 points, 372.9±56.7 points, and 295.2±42.2 points, respectively. There were many problems in these institutions, such as lack of personnel, low levels of standardization and information construction, weak clinical service ability of the service providers, and insufficient scientific research ability. Conclusion: In the future, the personnel training and guidance should be strengthened, the capacity building of maternal and child health care institutions should be enhanced, and the information construction should be improved, so as to the institutions can achieve better performance appraisal.

2022 Vol. 30　(9):　1955- [Abstract]（ 242 ） HTML (0 KB)  PDF  (0 KB)  ( 22 )

2022 Vol. 30　(9):　1963- [Abstract]（ 360 ） HTML (0 KB)  PDF  (0 KB)  ( 25 )

To investigate the correlation between the levels of human chorionic gonadotropin(HCG) and soluble human leukocyte differentiation antigen-C(sHLA-G) in embryo culture medium of women during in vitro fertilization- embryo transfer(IVF-ET) and their clinical pregnancy after assisted reproduction. Methods: The clinical data of 120 women who were being treated for infertility and underwent IVF-ET from February 2018 to May 2020 were analyzed retrospectively. Two embryos were transferred for each of 120 patients in every IVF-ET cycle. According to the clinical pregnant situation, 120 woen were divided into group A(60 women with successful pregnancy) and group B(60 women with pregnancy failure). Logistic regression model was used to analyze the risk factors affecting pregnancy of the women after IVF-ET. Spearman method was used to analyze the correlation between the HLA-G and HCG levels of the women and their pregnancy and the number of excellent embryos. Receiver operating characteristic curve(ROC) curve was used to analyze the efficacy of the levels of HLA-G and HCG of the women for predicting their pregnancy outcomes. Results: There were no significant differences in age, insemination method, infertility time, basal sex hormone level, Gn days, Gn total dosage, endometrial thickness, number of oocytes obtained, number of fertilization eggs, and number of embryos of the women between the two groups(P>0.05). The levels of HCG and SHLA-G in embryo culture medium and number of good embryos of the women in group A were significantly higher than those of the women in group B(all P<0.05). Multivariate logistic regression analysis showed that the SHLA-G and HCG levels, and the number of good embryos of the women after IVF-ET were the independent risk factors of their pregnancy(P<0.05). Spearman correlation analysis showed that the SHLA-G and HCG levels of the women were positively correlated with their pregnancy after IVF-ET(r=0.559, 0.682, all P<0.05) and their number of good embryos(r=0.602, 0.564, all P<0.05). ROC curve analysis showed that the area under the curve of HCG level and SHLA-G level for predicting clinical pregnancy were 0.823 and 0.894, respectively. The optimal cut-off value, the sensitivity, the specificity, and Youden index of HCG level for predicting clinical pregnancy were 1.29IU/L, 66.7%, 85.0%, and 0.517, respectively. The optimal cut-off value, the sensitivity, the specificity, and Youden index of SHLA-G level for predicting clinical pregnancy were 4.73 IU/ml, 78.3%, 90.0%, and 0.683, respectively. Conclusion: The levels of HCG and sHLA-G in embryo culture medium of the women after assisted reproduction have certain correlation with their early embryo development and clinical pregnancy, and which can be used as the important reference indicators for predicting pregnancy of the women after assisted reproduction.

2022 Vol. 30　(9):　1972- [Abstract]（ 334 ） HTML (0 KB)  PDF  (0 KB)  ( 30 )

To study the efficacy of low molecular weight heparin calcium for adjuvant treating women with recurrent abortion, and to study its effects on their cyclooxygenase 2(COX-2) and interleukin 33(IL-33) levels. Methods: 121 women with recurrent abortion were selected and were divided into two groups by random number table method from January 2019 to January 2021. 61 women in the study group were given aspirin combined with low molecular weight heparin calcium treatment, and 60 women in the control group were given aspirin only. The clinical efficacy, the changes of the levels of serum COX-2, IL-33, estradiol(E2), progesterone(P), chorionic gonadotropin(β-HCG), and D-dimer(D-D), the plasminogen activator inhibitor 1(PAI-1) and fibrinogen levels, and the incidence of adverse reactions of the women were compared between the two groups. Results: After treatment, the total effective rate(83.6%) of the women in the study group was significantly higher than that(66.7%) of the women in the control group. The serum levels of COX-2 and IL-33(525.47±67.19 U/L and 1318.15±398.61 ng/L) of the women in the study group were significantly higher than those of the women(444.94±76.05U/L and 1017.79±150.16 ng/L) in the control group. The serum E2, P, and β-hCG levels of the women in both groups had increased significantly, and which of the women in the study group was significantly higher than that of the women in the control group. The serum levels of D-D, PAI-1, and fibrinogen of the women in the two groups had decreased significantly, and which(121.34±26.55μg/L, 0.51±0.23 AU/ml, and 2.13±0.24 g/L) of the women in the study group were significantly lower than those(187.97±35.23μg/L, 0.95±0.28 AU/ml, and 4.11±0.35 g/L) of the women in the control group(all P<0.05). There was no significant difference in the total incidence of adverse reactions(8.2% vs. 11.7%) of the women between the two groups(P>0.05). Conclusion: Low molecular weight heparin calcium as adjuvant therapy for the women with recurrent abortion can improve the effect with good safety, which may be related to the effectively improvement of COX-2 and IL-33 levels.

2022 Vol. 30　(9):　1983- [Abstract]（ 352 ） HTML (0 KB)  PDF  (0 KB)  ( 23 )

To compare and analyze the effect of cervical cerclage and progesterone for treating singleton pregnant women with short cervix(<10 mm). Methods: 132 singleton pregnant women with short cervix(<10 mm) between June 2018 and June 2021 were collected retrospectively. These women were divided into group A(83 cervical cerclage treatment) and group B(49 women with progesterone treatment) according to different treatment methods. The cumulative delivery rate, the pregnancy status, the neonatal status, and the incidence of complications of the women were compared between the two groups. Results: There was no significant difference in the delivery mode of the women between the two groups(P>0.05). The cumulative delivery rate(81.9%) of the women during ≥37 gestational weeks in group A was significantly higher than that(63.3%) of the women in group B, the gestational weeks at delivery(36.6±2.1 weeks) and the gestational prolongation time(20.4±3.7 weeks) of the women in group A were significantly longer than those(35.6±2.3 weeks and 19.1±3.8 weeks) of the women in group B(all P<0.05). There was no significant difference in the neonatal survival rate between the two groups(P>0.05). The birth weight of neonates(2.8±0.4kg) in group A was significantly higher than that(2.5±0.4kg)in group B, the proportion of the neonates transferred in neonatal department(15.7%) in group A was significantly lower than that(34.7%) of the neonates in group B, and the neonatal Apgar score(8.0±1.4 points) in group A was significantly higher than that(7.5±1.2 points) in group B (P<0.05). There was no significant difference in the complication rate(10.2% vs.12.1%) of the women between the two groups(P>0.05). Conclusion: The effect of cervical cerclage for treating singleton pregnant women with short cervix＜10 mm is higher than that of progesterone treatment. The rational use of drugs for anti-infection and control uterine contraction, and regular monitoring vaginal secretions situation of these pregnant women can reduce their rate of infection after cerclage and can decrease their incidence of premature delivery.

2022 Vol. 30　(9):　1992- [Abstract]（ 318 ） HTML (0 KB)  PDF  (0 KB)  ( 25 )

To explore the value of P16 autoantibody(P16 IgG) level combined with HPV detection for diagnosing high-grade squamous intraepithelial lesion(HSIL), and to evaluate the prognosis of these women. Methods: A total of 85 women with HSIL who wanted surgery treatment were selected in observation group, and 85 healthy women who wanted cervical examination were selected in control group from March 2019 to March 2020. The serum P16 IgG and HPV-DNA levels of the women in the observation group were detected before operation, and which of the women in the control group were detected during physical examination, respectively. The women in the observation group were followed up for 12 months after operation and were divided into group A(women with progression of HSIL) and group B(women with stabilization of HSIL). The values of the serum P16 IgG and HPV-DNA levels of the women in the observation group for diagnosing their HSIL and for evaluating their prognosis were analyzed. Results: The levels of preoperative serum P16 IgG(1.26±0.30) and HPV-DNA(2.12±0.69) of the women in the observation group were significantly higher than those(1.06±0.18 and 0.41±0.17) of the women in the control group. The serum P16 IgG level(1.11±0.20) and the HPV-DNA level(0.85±0.24) of the women in the observation group after operation had decreased significantly, and which were significantly  higher than those(1.06±0.18 and 0.41±0.17) of the women in the control group after operation(all P<0.05). There were 28 women with progression or reoccurrence of HSIL in the observation group during the follow up for 12 months after operation. The serum P16 IgG and HPV-DNA levels of the women in group A were significantly higher than those of the women in group B. The serum P16 IgG and HPV-DNA levels of the women in group A after operation had decreased significantly, and which were significantly lower than those of the women in group B after operation(P<0.05). There was a positive correlation between the serum P16 IgG level of the women in observation group and their HPV-DNA level(r=0.784, 0.803, P<0.001). The area under curve(AUC) of the P16 IgG level and HPV-DNA level for diagnosing HSIL were 0.878 and 0.736, the best cut-off values of the P16 IgG level and HPV-DNA level for diagnosing HSIL were 1.21 and 2.11, the sensitivity of the P16 IgG level and HPV-DNA level for diagnosing HSIL were 91.3% and 81.4%, and the specificity of the P16 IgG level and HPV-DNA level for diagnosing HSIL were 78.8% and 69.6%. The AUC, the sensitivity and the specificity of the combined detection of P16 IgG and HPVDNA levels for diagnosing HSIL were 0.933, 97.7%, and 92.6%, respectively. The AUC of P16 IgG and HPV-DNA levels of the women for assessing prognosis of HSIL were 0.771 and 0.704, the best cut-off values of P16 IgG and HPV-DNA levels of the women for assessing prognosis of HSIL were 1.10 and 0.86, the sensitivity of P16 IgG and HPV-DNA levels of the women for assessing prognosis of HSIL were 86.5% and 79.4%, and the specificity of P16 IgG and HPVDNA levels of the women for assessing prognosis of HSIL were 71.7% and 66.8%. The AUC, the sensitivity and the specificity of the combined detection of P16 IgG and HPV-DNA levels for assessing prognosis of HSIL were 0.897, 91.7%, and 90.1%, respectively. Conclusion: The abnormally elevated levels of P16 IgG and HPV-DNA are related to the cervix HSIL lesions of the women, and the detections of P16 IgG and HPV levels can be used as auxiliary indicators for the early diagnosis and prognosis evaluation of the cervix HISL.

2022 Vol. 30　(9):　2001- [Abstract]（ 343 ） HTML (0 KB)  PDF  (0 KB)  ( 25 )

To investigate the effects of esketamine for postoperative analgesia of obese parturient(BMI ≥ 30 kg/m2) on their pain control and depression after cesarean section. Methods: A total of 82 obese parturients who had undergone cesarean section were selected and randomly divided into two groups(41 cases in each group) from January 2020 to December 2021. The parturients in the research group were given 45mg estazolam combined with 50μg sufentanil and 10mg azasetron for postoperative analgesia, and the parturient in the control group were given 100μg sufentanil combined with 10mg azasetron for postoperative analgesia. All the parturient were followed up for 8 weeks after operation. The intraoperative related indicators, the NRS analgesic score, the postpartum depression score(EPDS), the analgesic-related adverse reactions, the sleep quality, the postpartum anxiety, and the recovery quality of the parturient in the two groups were observed. Results: All 82 parturient had completed the follow-up. There were no significant differences in the operation time, the anesthesia duration, and the intraoperative blood loss of the parturient between the two groups(P>0.05). The resting NRS scores of the parturient in the research group at 2h, 6h, 12h, 24h, and 48h after operation were significantly lower than those of the parturient in the control group, and the locomotor NRS scores of the parturient in the research group at 6h, 12h, 24h and 48h after operation were also significantly lower than those of the parturient in the control group. The EPDS scores of the parturient in the research group in 1, 3, 6 and 8 weeks after operation were significantly lower than those of the parturient in the control group(all P<0.05). Compared with those of the parturient in the control group, the parturient in the research group had better sleep quality, lower GAD-7 score(5.73±2.16 points vs. 7.49±2.58 points), and higher QOR-15 score(122.47±29.51 points vs. 108.45±25.66 points)(all P>0.05).  Conclusion: Esketamine can reduce the postoperative pain degree of the obese parturient, relieve their postpartum depression, and promote their recovery quality, with good safety.

2022 Vol. 30　(9):　2012- [Abstract]（ 674 ） HTML (0 KB)  PDF  (0 KB)  ( 29 )

To investigate the influence of different doses of melatonin for treating patients with laparoscopic hysterectomy under general anesthesia on their postoperative pain, sleep, and inflammation indicators. Methods: A total of 96 patients who wanted laparoscopic hysterectomy under general anesthesia were selected and were randomly divided into group A(48 cases with 5 mg melatonin treatment before operation) and group B(48 cases with 10 mg melatonin treatment before operation). The sleep quality evaluated by St.Mary's Hospital Sleep Questionnaire(SMH) and Pittsburgh Sleep Quality Index(PSQI), the alleviate situation of pain evaluated by Visual Analogue Scale(VAS) and Numerical Rating Scale(NRS), the levels of inflammatory indicators, such as tumor necrosis factor-α(TNF-α), interleukin-6(IL-6), and C-reactive protein(CRP), the serum central nervous system specific protein(S100-β) level, the cognitive function evaluated by MiniMental State Examination(MMSE), and the rate of adverse reactions of the patients were compared between the two groups. Results: The SMH score and MMSE score of the patients in the two groups on the 1st day after operation were significantly lower than those 1 day before operation, but which of the patients in group B were significantly higher than those of the patients in group A. The SMH score and MMSE score the patients in the two groups on the 7th day after operation were significantly higher than those on the 1st day after operation(P<0.05), but which of the patients had no significant difference between the two groups. The PSQI score of the patients in the two groups on the 1st day after operation was significantly higher than that 1 day before operation, and which of the patients in group B was significantly lower than that of the patients in group A(P<0.05). The PSQI scores of the patients in the two groups on the 7th day after operation were significantly lower than that on the 1st day after operation(P< 0.05), but which of the patients had no significant difference between the groups. The VAS and NRS scores of the patients in group B on the 1st, the 2 second, and the 3rd day after operation were significantly lower than those of the patients in group A. The serum levels of TNF-α, IL-6, and CRP the patients in the two groups on the 1st day after operation were significantly higher than those before operation, and which of the patients in group B were significantly lower than those of the patients in group A. The serum S100β level of the patients in the two groups on the 1st day after operation were significantly higher than that before operation, and the increase degree of the patients in group B was significantly lower than that of the patients in group A(all P< 0.05). There were no any patients with adverse reactions, such as pruritus, reflux aspiration, respiratory depression, hypoxemia, nausea, or vomiting in the two groups. Conclusion: Compared with those of 5mg melatonin, 10mg melatonin for treating the patients before laparoscopic hysterectomy under general anesthesia can more effectively reduce the inflammation and pain of the patients, reduce the changes of their postoperative cognitive function and sleep quality, with medication safety.

2022 Vol. 30　(9):　2022- [Abstract]（ 407 ） HTML (0 KB)  PDF  (0 KB)  ( 26 )

To observe the effects of family participation combined with empowerment education intervention for treating pregnant women with severe preeclampsia during the third trimester of pregnanct on their psychological states and maternal and neonatal outcomes. Methods: 114 pregnant women with severe preeclampsia during the third trimester of pregnancy were selected and were divided into control group(n=56) and observation group(n=58) according to nursing method between May 2018 and May 2021. The women in the two groups were given routine nursing.  The women in the observation group were given family participation combined with empowerment education intervention additionally. The degree of family perceived social support, the psychological states, and the maternal and neonatal outcomes of the women were compared between the two groups. Results: After intervention, the score of perceived social support scale(22.41±2.12 points) of the women in the observation group was significantly higher than that(20.26±2.47 points) of the women in the control group. The scores of toughness, optimism, and strength by Connor-davidson Resilience Scale(CD-RISC) of the women in the observation group were significantly higher than those of the women in the control group. The scores of self-rating anxiety scale(31.10±4.16 points) and self-rating depression scale(24.42±4.46 points) of the women in the observation group were significantly lower than those(35.62±4.08 points, 28.26±4.47 points) of the women in the control group(P<0.05). The gestational weeks of pregnancy termination(32.3±1.4 weeks) and the expected treatment days(10.5±2.7 days) of the women in the observation group were significantly longer than those(31.4±1.5 weeks and 8.3±2.4 days) of the women in the control group. The vaginal delivery rate(93.1%) of the women in the observation group was significantly higher than that(75.0%) of the women in the control group. The neonatal Apgar score(7.0±0.4 points) and the neonatal body weight(3.1±0.7kg) in the observation group were significantly higher than those(6.7±0.4 points and 2.9±0.5kg) in the control group, and the neonatal complication rate(8.6%) in the observation group was significantly lower than that(30.4%) in the control group(all P<0.05). Conclusion: Family participation combined with empowerment education intervention can improve social-family support, psychological states, and maternal and neonatal outcomes of the pregnant women with severe preeclampsia during the third trimester of pregnancy.

2022 Vol. 30　(9):　2031- [Abstract]（ 518 ） HTML (0 KB)  PDF  (0 KB)  ( 25 )

To explore the clinical application of chromosomal analysis microarray(CAM) technology for fetus with congenital heart disease(CHD) and extracardiac malformation screened by ultrasonic STIC technology in the prenatal diagnosis. Methods: 380 high-risk pregnant women with fetal heart examination were selected as the study subjects from January 2020 to January 2021. The situation of fetal CHD was screened by two-dimensional ultrasound and ultrasonic STIC technology, and which was compared with the results of anatomical pathology and postpartum follow-up for analyzing the accuracy of two-dimensional ultrasound and ultrasonic STIC technology. The detections of pathogenic chromosome copy number variation(CNV) of the fetus with CHD and extra-cardiac malformation were compared by between CAM technology and ultrasonic STIC technology, and their clinical values were analyzed. Results: Among 380 highrisk pregnant women, 104 fetuses with CHD were detected, and a total of 301 cardiac structural abnormalities were found. The anatomical pathology and postpartum follow-up results were as the gold standard, the sensitivity and the specificity of two-dimensional ultrasound for diagnosing fetal CHD were 91.4% and 98.9%, and the sensitivity and the specificity of ultrasonic STIC for diagnosing fetal CHD were 96.2% and 99.3%, and the two kinds of ultrasonic technology for diagnosing fetal CHD had good consistency(Kappa=0.919, 0.960). There were no significant differences in the coincidence rate, the missed detection rate, and the misdiagnosis rate for diagnosing fetal CHD between the two kinds of ultrasonic technology(P>0.05), but there was significant difference in the coincidence rate for diagnosing fetal cardiac structural abnormalities(χ2=61.499, P<0.05). Among 100 fetal CHD with abnormal karyotype analysis, there were 66(66.0%) fetuses with single type CHD and 34(34.0%) fetuses with complex type CHD. Among the fetuses with complex type CHD, there were 23(23.0%) fetuses with CHD and extracardiac malformation, 15(15.0%) fetuses with single CHD and extracardiac malformation, and 6 fetuses with pathogenic CNV. In the fetuses with complex type CHD, there were 8(8.0%) fetuses with CHD combined with extracardiac malformation and without pathogenic CNV. In the fetuses with single type CHD, there were 77(77.0%) CHD fetuses with without extracardiac malformation, 51(51.0%) CHD fetuses without extracardiac malformation, and 6 fetuses with pathogenic CNV. In the 26(26.0%) fetuses with complex type CHD and extracardiac malformation, there were 5 fetuses with pathogenic CNV. Conclusion: Ultrasonic STIC technology has good diagnostic value for fetal CHD and extracardiac abnormalities during prenatal diagnosis, and CMA technique has good detectable rate for chromosome microduplication and microdeletion.

2022 Vol. 30　(9):　2040- [Abstract]（ 348 ） HTML (0 KB)  PDF  (0 KB)  ( 26 )

To investigate the correlation between the temperament optimistic situation of patients with cervical cancer caused by cervical high risk HPV (hr-HPV) infection and their life satisfaction, coping style, hope level, anxiety, and depression. Methods: 126 patients with hr-HPV infection were surveyed and analyzed from September 2018 to December 2021. The patients were divided into group A (66 patients with dispositional optimism) and group B (60 patients with dispositional optisimism) according to the temperament optimistic situation of the patient. The scores of life satisfaction, anxiety, depression, coping style, and hope level of the patients were compared between the two groups. The correlation between the temperament optimistic situation of these patients and their life satisfaction, anxiety, depression, coping style, and hope level was analyzed. Results: The scores of SWLS (29.33±3.41 points), seeking support (2.67±0.15 points), optimism(2.58±0.26 points), emotional catharsis (2.41±0.25 points), direct face (2.33±0.25 points), and hope level (54.08±6.58 points) of the patients in group A were significantly higher than those(10.22±2.17 points, 0.09±0.21 points, 1.87±0.23 points, 1.69±0.17 points, 27.76±3.69 points, and 1.37±0.18 points) of the patients in group B. The scores of self-dependence(0.87±0.12 points), fatalism (0.69±0.07 points), avoidance(0.75±0.08 points), appease(0.56±0.07 points), anxiety(16.35±2.33 points), and depression(18.33±2.64 points) of the patients in group A were significantly lower than those(1.36±0.1 8 points, 1.55±0.16 points, 1.23±0.17 points, 1.33±0.15 points, 35.64±5.11 points, 38.29±4.23) of the patients in group B (all P<0.05). The Pearson correlation analysis showed that, the temperament optimistic situation of the patients was positively correlated their life satisfaction, coping style, and hope level, and was negatively correlated with their anxiety and depression (all P<0.05). Conclusion: The dispositional optimism of the patients with cervical cancer caused by cervical hr-HPV infection is associated with their life satisfaction, anxiety, depression, coping style, and hope level. It is suggested that intervention based on above factors may be expected to improve the dispositional optimism of the patients in clinic and improve the positively treating disease of the patients.

2022 Vol. 30　(9):　2050- [Abstract]（ 336 ） HTML (0 KB)  PDF  (0 KB)  ( 23 )

2022 Vol. 30　(9):　2060- [Abstract]（ 330 ） HTML (0 KB)  PDF  (0 KB)  ( 23 )

To analyze the correlation between the expression of CXCR5+CD4+T cells of infertility patients with endometriosis (EMS) and their pathogenesis and autoantibodies. Methods: 78 patients with EMS and infertility were selected in observation group, and 50 patients with EMS only were selected in control group from January 2019 to February 2021. The levels of endometrial antibody (EMAb), anti-ovarian antibody (AoAb), anti-sperm antibody (AsAb), thyroid autoantibody (ATA), anti-human chorionic gonadotropin antibody (AhCGAb), anti-β-2 glycoprotein 1 (α-β-2GP1), and CXCR5+CD4+T cell of the patients in the two groups were detected, and the correlation of which was analyzed. Results: The levels of AsAb (3.32±0.54), AoAb (2.94±0.28), EMAb (3.21±0.62), AhCGAb (3.64±0.59), α-β-2GP1 (3.28±0.61), and ATA (2.81±0.23) of the patients in the observation group were significantly higher than those (1.43±0.39, 2.13±0.34, 1.32±0.43, 2.03±0.48, 1.29±0.57, and 2.01±0.21) of the patients in the control group, and the CXCR5+CD4+T cell level (23.19±5.91%) of the patients in the observation group was significantly higher than that (9.34±2.88 %) of the patients in the control group (all P<0.05). The level of CXCR5+CD4+T cells of the patients with EMS was positively correlated with their incidence of infertility. The levels of AoAb, AsAb, EMAb, ATA, α-β-2GP1, and AhCGAb of the patients in the observation group were positively correlated with their CXCR5+CD4+T cells level (all P<0.05). Conclusion: The CXCR5+CD4+T cells level and the related antibody level of the patients with EMS and infertility all increase abnormally, and there is a positive correlation between the expression of CXCR5+CD4+T cells level of the infertility patients with EMS and their autoantibody level.

2022 Vol. 30　(9):　2068- [Abstract]（ 397 ） HTML (0 KB)  PDF  (0 KB)  ( 26 )

2022 Vol. 30　(9):　2076- [Abstract]（ 318 ） HTML (0 KB)  PDF  (0 KB)  ( 23 )

To study the situation of adverse maternal and infant outcomes of pregnant women with group B streptococcal (GBS) infection during the third trimester of pregnancy. Methods: 80 pregnant women with positive GBS in vaginal secretion during the third trimester of pregnancy (35-37 gestational weeks) were selected in observation group and 80 pregnant women with negative GBS in vaginal secretion were selected in control group from June 2019 to September 2020. The maternal and infant outcomes in the two groups were followed up. Results: The rates of the premature rupture of membranes (55.0%) and the total adverse pregnancy (72.5%) of the women in the observation group were significantly higher than those (35.0% and 45.0%) of the women in the control group, and the rates of the neonatal asphyxia (8.8%) and the total adverse events (20.0%) in the observation group were also significantly higher than those (0 and 2.5%) in the control group (all P<0.05). Conclusion: The pregnant women with prenatal GBS infection can increase the incidence of their adverse pregnancy, leading to the increase of neonatal adverse outcomes. Therefore, it is necessary to strengthen the detection of GBS infection of the pregnant women during the third trimester of pregnancy, and to intervene timely for reducing the incidence of adverse pregnancy.

2022 Vol. 30　(9):　2086- [Abstract]（ 399 ） HTML (0 KB)  PDF  (0 KB)  ( 22 )

To explore the expressions and clinical significance of Toll-like receptor 4 (TLR4) and NOD-like receptor protein 3 (NLRP3) inflammasomes in peripheral blood of women with unexplained recurrent spontaneous abortion (URSA). Methods: 65 women with URSA were enrolled in observation group and 30 women with normal early pregnancy who wanted induced abortion were selected in control group between June 2019 and June 2021. The general data, the levels of β-human chorionic gonadotropin (β-hCG) and progesterone (P), and the expressions of TLR4 and NLRP3 inflammasomes in peripheral blood of the women were compared between the two groups. Pearson correlation analysis was used to analyze the correlation between the expressions of TLR4 and NLRP3 inflammasomes in peripheral blood of the women in the observation group and their levels of β-hCG and P. Logistics regression equation was used to analyze the correlation between the expressions of peripheral blood TLR4 and NLRP3 inflammasomes of the women in the observation group and their URSA. Results: The levels of serum β-HCG and P of the women in the observation group were significantly lower than those of the women in the control group, and the expressions of TLR4 mRNA, NLRP3 mRNA, ASC mRNA, and caspase-1 mRNA in peripheral blood of the women in the observation group were significantly higher than those of the women in the control group (all P<0.05). The expressions of TLR4 mRNA, NLRP3 mRNA, ASC mRNA, and caspase-1 mRNA in peripheral blood of the women in the observation group were correlated with theirβ-HCG and P levels (P<0.05). Logistic regression analysis showed that the expressions of TLR4 mRNA, NLRP3 mRNA, ASC mRNA, and caspase-1 mRNA in peripheral blood of the pregnant women were the related factors of their URSA (P<0.05). Conclusion: The expressions of TLR4 mRNA, NLRP3 mRNA, ASC mRNA, and caspase-1 mRNA in peripheral blood of the women with URSA increase abnormally. The immune inflammatory response may be involved in the process of URSA of the women.

2022 Vol. 30　(9):　2094- [Abstract]（ 296 ） HTML (0 KB)  PDF  (0 KB)  ( 23 )

To explore the effect of uterine strap suture (uterine B-lynch suture) or uterine artery ascending branch ligation for treating women with postpartum hemorrhage (PPH). Methods:The clinical data of 92 women with PPH from October 2016 to October 2020 were analyzed retrospectively. These women were divided into group A (46 women with uterine B-lynch suture) and group B (46 women with uterine artery ascending branch ligation). The hemostatic efficacy, the perioperative indicators, such as the intraoperative blood loss, the blood loss in 24h after surgery, the blood transfusion volume, the surgical time, and the hospital stay time, the postoperative prognosis status, such as puerperalism, incomplete elimination of lochia, incision infection, intrauterine adhesions, adverse uterine involution, and other complications, and the ovarian function indexes, such as anti-Müllerian hormone (AMH), estrogen (E2), luteinizing hormone (LH), and follicle stimulating hormone (FSH) before surgery and in 1 week after surgery, of the women were compared between the two groups. Results: There were no significant differences in the total effective rate of hemostasis (95.7% vs. 93.5%), the intraoperative blood loss (1554.6±281.7 ml vs.1568.6±287.2 ml), the blood loss in postoperative 24h (225.6±55.6 ml vs.236.2±61.1 ml), the blood transfusion volume (1215.3±129.2ml vs.1239.6±118.7ml), the operation time (58.6±9.1min vs.60.8±9.2min), the hospital stay time (7.3±2.1d vs.8.1±2.3d), the incidence of puerperal disease (13.0% vs.15.2%), the rate of incomplete elimination of lochia (6.5% vs.4.0%), the rate of incision infection (2.2% vs.4.4%), the rate of intrauterine adhesion (0 vs.4.4%), and the rate of adverse uterine involution (82.6% vs.87.0%) of the women between the two groups (all P＞0.05). The serum AMH and E2 levels of the women in the two groups in 1 week after surgery had increased compared with those before surgery operation, while the LH and FSH levels of the women in the two groups had decreased (all P<0.05), but which of the women had no significant difference between the two groups (P＞0.05). Conclusion: Uterine B-Lynch suture for treating the women with PPH can achieve the same hemostatic effect as that of uterine artery ascending branch ligation. Both uterine B-Lynch suture and uterine artery ascending branch ligation will not increase the complications, and has good postoperative ovarian function recovery.

2022 Vol. 30　(9):　2102- [Abstract]（ 335 ） HTML (0 KB)  PDF  (0 KB)  ( 26 )

To explore the changes of intestinal flora, vitamin D level, and glucose and lipid metabolism of pregnant women with gestational diabetes mellitus (GDM), and to analyze their values for predicting adverse pregnancy outcomes of these women. Methods: A total of 95 pregnant women with GDM who underwent routine pregnancy examinations from January 2018 to January 2021 were selected as in research group, and 80 healthy pregnant women were selected in control group during the same period. The change of the intestinal flora, the levels of serum 25(OH)D, the blood glucose indicators, such as fasting blood glucose (FBG), 2h postprandial blood glucose (2hPG), glycosylated hemoglobin (HbA1c), fasting insulin (FINS), and insulin resistance index by homeostasis model assessment of insulin resistance (HOMA-IR), and the blood lipid indexes, such as triglycerides (TG), total cholesterol (TC), high-density lipoprotein cholesterol (HDL-C), and low-density lipoprotein cholesterol (LDL-C), of the women in the two groups were detected. The pregnancy outcomes of the women in the two groups were analyzed. Results: The proportions of intestinal enterobacteria and yeast of the women in the research group were significantly higher than those of the women in the control group, while the proportions of intestinal bifidobacteria, lactobacillus, and bacteroides of the women in the research group were significantly lower than those of the women in the control group. The levels of FBG, 2hPG, HbA1c, FINS, TG, and LDL-C, and the HOMA-IR value of the women in the research group were significantly higher than those of the women in the control group. The levels of 25-(OH) D of the women in the research group was significantly lower than that of the women in the control group. The proportions of adverse pregnancy outcomes, such as pregnancy-induced hypertension, postpartum hemorrhage, premature delivery, and puerperal infection of the women in the research group were significantly higher than those of the women in the control group (all P<0.05). The analysis of receiver operator characteristics curve showed that the intestinal microbiota, the vitamin D, and glucose and lipid metabolism levels of the women with GDM had impact on their pregnancy outcomes. The FBG and 25-(OH) D levels, and the HOMA-IR value of the women with GDM had certain predictive values for their adverse pregnancy outcomes, and the Youden index of which were 0.562, 0.517, and 0.573, respectively, the sensitivity of which were 0.842, 0.737, and 0.895, respectively, and the specificity of which were 0.720, 0.780, and 0.678, respectively. Conclusion: Compared with those of the healthy pregnant women, the proportions of beneficial bacteria in the intestines of the pregnant women with GDM reduce significantly, their vitamin D level decreases, and their glucose and lipid metabolism are imbalanced, which leads to the increase of adverse pregnancy outcomes. It is suggested that the levels of vitamin D, and glucose and lipid of the pregnant women with GDM should be detected early, and the changes of intestinal flora of these women should be paid attention to in order to reduce the incidence of their adverse pregnancy outcomes.

2022 Vol. 30　(9):　2111- [Abstract]（ 362 ） HTML (0 KB)  PDF  (0 KB)  ( 28 )

To investigate the correlation of the levels of the serum anti-Müllerian hormone (AMH) and integrin β1 (ITGβ-1) of pregnant women with preeclampsia during the second and the third trimester of pregnancy and their uterine spiral artery blood flow parameters and vascular endothelial cell (VEC) injury indictors. Methods: 96 pregnant women with preeclampsia during the second and the third trimester of pregnancy were selected in observation group and another 96 healthy pregnant women were selected in control group from March 2019 to March 2021. The serum AMH and ITGβ-1 levels, and the values of uterine spiral artery blood flow parameters, such as PI, RI, and S/D, and the levels of endothelial progenitor cells (EPCs) and angiogenic T cells (Tang) of the women in both groups were measured, and the correlation of which each other was analyzed. Results: The levels of AMH (1.62±0.15 ng/ml) and ITGβ-1 (0.05±0.02) of the women in the observation group were significantly lower than those (2.44±0.17 ng/ml and 0.18±0.03) of the women in the control group. The values of the uterine spiral artery blood flow parameters of the women in the observation group were significantly higher than those of the women in the control group. The levels of EPCs (0.08±0.03%) and Tang (47.31±8.57%) of the women in the observation group were significantly lower than those (0.16±0.05 % and 53.61±7.95%) of the women in the control group (all P<0.05). The levels of AMH and ITGβ-1 of the women were negatively correlated with their uterine spiral artery blood flow parameters values, and were positively correlated with their VEC injury indexes values (all P<0.05). Conclusion: The levels of serum AMH and
ITGβ-1 of the pregnant women with preeclampsia during the second and the third trimester of pregnancy change abnormally, which are correlated with their uterine spiral artery blood flow parameters and their VEC injury. 

2022 Vol. 30　(9):　2120- [Abstract]（ 296 ） HTML (0 KB)  PDF  (0 KB)  ( 25 )

To analyze the situations of the chronic hepatitis B virus (HBV) load during pregnancy and postpartum hepatitis occurrence of women, and neonatal HBV infection. Methods: The clinical data of 208 pregnant women with chronic HBV carriers from January 2019 to October 2020 were analyzed retrospectively. The women with HBV DNA ≥106 copies/mL before delivery were included in group A, and the women with HBV DNA<106 copies/mL were included in group B. The adverse outcomes of maternal and infant, the abnormal liver function rate, and the hepatitis occurrence of the women in the two groups were recorded. In the group A, the women with HBV DNA ≥500 copies/mL were included in group A1, and the women with HBV DNA <500 copies/mL were included in group A2. The situation of alanine aminotransferase (ALT) elevation of the women with different loads of HBV in the 42th d after delivery was analyzed, and the positive rates of HBV DNA in cord blood immediately after birth and in infant venous blood in the 6th month after birth was also analyzed. Results: The overall incidence of adverse outcomes of maternal and infant (33.8%), the neonatal intrauterine infection rate (20.9%), the abnormal liver function rate on the postpartum 42th day (36.7%), and the hepatitis rate (15.8%) of the women in group A were significantly higher than those (18.8%, 1.5%, 21.7%, and 5.8%) of the women in group B. The ALT level of the women in group A1 was significantly higher than that of the women in group A2 (all P<0.05). Spearman rank correlation analysis showed that the HBV DNA load of the women in group A was positively correlated with their ALT level (P<0.05). The positive rates of HBV DNA in neonatal umbilical cord blood at birth (38.1%) and in infants’ venous blood in the 6th month after birth (7.9%) in group A1 were significantly higher than those (6.6% and 0) in group A2 (all P<0.05). Conclusion: The HBV DNA load of the pregnant women with chronic HBV carriers before delivery is closely related to the degree of their abnormal liver function and their adverse maternal and infant outcomes. The pregnant women with high HBV DNA load have higher risk of mother-to-child vertical transmission of HBV and have higher risk of failure to blockade of HBV, thus it is necessary to perform proper clinical treatment for these women during the third trimester of pregnancy so as to reduce the adverse effect of high HBV load.

2022 Vol. 30　(9):　2129- [Abstract]（ 351 ） HTML (0 KB)  PDF  (0 KB)  ( 26 )

To compare the effect of disposable balloon cervical dilator used in induction of labor of full-term pregnant women with different amniotic fluid volumes. Methods: 116 full-term pregnant women who had undergone induction of labor by disposable balloon cervical dilator from August 2019 to September 2021 were selected retrospectively. According to the amniotic fluid index (AFI) value, these women were divided in group (26 women with the AFI value below 5cm), group B (42 women with the AFI value in 5-8cm), and group C (48 women with the AFI value over 8cm), respectively. The effective rate of promoting cervical ripening, the pregnancy outcomes, and the neonatal outcomes of the women were compared among three groups. Results: The effective rate (53.9%) of promoting cervical ripening of the women in group A was significantly lower than that (71.4%) of the women in group B and that (85.4%) of the women in group C (P<0.05). There were no significant differences in the postpartum blood loss, the duration of first stage of labor, and the complications rate of induced labor of the women among the three groups (P＞0.05). The rate of natural delivery of the women in group A (19.2%) or in group B (33.3%) were significantly lower than that (47.9%) of the women in group C, and the rate of cesarean section of the women in group A (53.9%) or in group B (47.6%) were significantly higher than that (27.1%) in group C. The Apgar score at 1min of newborns after birth in group A (7.92±0.27 points) or in group B (7.85±0.32 points) was significantly lower than that (9.92±0.26 points) of the women in group C (all P<0.05). There was no significant difference in the incidence of neonatal jaundice among the three groups (P＞0.05). The incidences of the neonatal low weight, fetal distress, asphyxia, and pneumonia in group A were significantly higher than those in group B and in group C (P<0.05). Conclusion: The value of AFI has significant impact on the outcomes of induced labor by bisposable balloon cervical dilator of full-term pregnant women. The lower value of AFI, the poorer pregnancy outcomes of the women is. AFI of the pregnant women can be used as a reference index of the for evaluating their pregnancy outcomes in the clinic.

2022 Vol. 30　(9):　2137- [Abstract]（ 231 ） HTML (0 KB)  PDF  (0 KB)  ( 25 )

To investigate the expression of serum long non-coding RNA-OIS1 (lncRNA-OIS1) of patients with cervical cancer, and to study its correlation with the cervical human papillomavirus (HPV) infection of the patients. Methods: From February 2020 to December 2021, 66 patients with cervical cancer were included in group A, 75 patients with cervical intraepithelial neoplasia were included in group B, and 72 patients with cervicitis were included in group C. The expression level of serum lncRNA-OIS1 of the patients in the three groups was detected by quantitative real-time PCR (qRT-PCR). HPV DNA detection kit and HPV E6/E7 mRNA kit were used to detect HPV positive expression and HPV E6/E7 mRNA load in cervical exfoliated cells of these patients. Spearman correlation analysis was performed to analyze the correlation between the serum lncRNA-OIS1 expression level of the patients with cervical cancer and their cervical HPV infection. Multivariate Logistic regression was performed to analyze the risk factors affecting the cervical cancer occurrence. Receiver operating characteristic (ROC) curve was performed to analyze the diagnostic value of serum lncRNA-OIS1 level for cervical cancer. Results: The expression levels of lncrNA-OIS1 of the patients in group C, in group B, and in group A were 1.01±0.17, 0.82±0.16, and 0.69±0.18, respectively, which had decreased gradually. The positive expression rates of HPV of the patients in group C, in group B, and in group A were 26.4%, 45.3%, and 100.0%, respectively, which had increased gradually, and the high load rate of HPV E6/E7 mRNA of the patients in group C, in group B, and in group A were 19.4%, 37.3%, 100.0%, respectively, which had increased gradually (all P<0.05). Spearman correlation analysis showed that the expression level of lncrNA-OIS1 of the patients with cervical cancer was negatively correlated with their HPV positive expression and their HPV E6/E7 mRNA load (r=-0.526, -0.489, all P=0.000). The low expression of lncrNA-OIS1, the positive HPV, and the high HPV E6/E7 mRNA load of the patients were the risk factors of their cervical cancer (P<0.05). The area under ROC curve of serum lncrNA-OIS1 level of the patients for diagnosing their cervical cancer was 0.892. Conclusion: The expression level of serum lncRNA-OIS1 of the patients with cervical cancer decreases, which is closely related to their cervical HPV infection. It has certain value for diagnosing cervical cancer and can be used as a biomarker for predicting cervical cancer.

2022 Vol. 30　(9):　2145- [Abstract]（ 787 ） HTML (0 KB)  PDF  (0 KB)  ( 26 )

To investigate the risk factors of spontaneous preterm birth of women with twin pregnancies. Methods：The clinical data of the women with twin pregnancies who had delivered in the fourth hospital Shijiazhuang from June 2015 to June 2020 were analyzed retrospectively. The women with preterm premature rupture of membranes, and iatrogenic preterm birth, and incomplete clinical data were excluded from this research. 298 women delivered during 28-36+6 gestational weeks were included in group A, and 1367 women delivered during ≥37 gestational weeks were included in group B. The clinical data of the women were compared between the two groups. The risk factors of spontaneous preterm delivery of the women with twin pregnancies were analyzed. Results: Logistic regression analysis showed that, the age <25 years old at delivery (OR=1.691, 95%CI 1.108-2.580), the history of preterm delivery (OR=4.828, 95%CI 1.28418.161), the history of early pregnancy (OR=1.592, 95%CI 1.284-18.161), the history of preventing miscarriage during the first trimester of pregnancy (OR=1.592, 95%CI 1.184-2.142), the monochorionic (OR=2.82, 95%CI 1.972-4.034), the vaginitis (OR=2.047, 95%CI 1.049-3.995), the anemia (OR=2.633, 95%CI 1.900-3.648), the cervical insufficiency (OR=5.961, 95%CI 2.986-11.901), the uterine malformation (OR=7.843, 95%CI 1.323-46.487) of the women with twin pregnancies were the risk factors of their spontaneous preterm birth. The twin pregnancies women with pre-pregnancy weight 55-65kg had less weight gain during pregnancy than those women with pre-pregnancy weight ≤55kg (OR=1.711, 95%CI 1.147-2.466), and the women with pre-pregnancy weight > 65kg had less weight gain during pregnancy than those women with pre-pregnancy weight ≤55kg (OR=2.31, 95%CI 1.356-3.783), and which were the risk factors of their spontaneous preterm birth. Compared with those twin pregnancies women with ≤70kg at delivery, the women with weight 70-85kg (OR=0.373, 95%CI 0.2540.547), and the women with weight >85kg (OR=0.229, 95%CI 0.134-0.392) of the women were the risk factors of their spontaneous preterm birth. Conclusion: The age <25 years old, the history of preterm birth, the history of preventing miscarriage during the first trimester of pregnancy, the monochorionic, the vaginitis, the anemia, the cervical insufficiency, the uterine malformation, the less weight gain during pregnancy, and other high-risk factors of the women with twin pregnancies should be paid more attention to, and based on which to formulate the individualized intervention measures, so as to improve the maternal and infant prognosis.

2022 Vol. 30　(9):　2154- [Abstract]（ 298 ） HTML (0 KB)  PDF  (0 KB)  ( 22 )

Previous cesarean scar diverticulum of uterus refers to low echo triangle or caesarean scar defect or diverticulum of non-pregnant women with previous history of cesarean section examined by transvaginal ultrasound or sonohysterography. The cesarean scar diverticulum of uterus is a long-term complication after cesarean section, and the main pathological features of which are endometrial hyperemia, lymphocytes infiltration, and telangiectasia. Studies have shown that the changes in the quality of uterine mucus, the accumulation of uterine fluid, and the local chronic inflammation of diverticulum are the main mechanisms of reducing sperm quality and affecting embryo implantation. In recent years, with the adjustment of China's fertility policy, more women have reproductive requirement, and the fertility function of some infertility women caused by uterine scar diverticulum can recover after surgery. This article review the research progress on the influencing factors of uterine scar diverticulum formation, diagnosis, infertility related mechanism, treatment, evaluation of surgical effect, and timing of postoperative fertility.

2022 Vol. 30　(9):　2165- [Abstract]（ 272 ） HTML (0 KB)  PDF  (0 KB)  ( 27 )