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Abstract To analyze the effects of trace elements for treating endothelial progenitor cells(EPCs) of pregnant women with preeclampsia and abnormal glucose and lipid metabolism on the proliferation and migration activity of these EPCs. Methods: The morning fasting venous blood samples were collected from 20 pregnant women with preeclampsia and abnormal glucose and lipid metabolism between November 2018 and November 2021 and the EPCs in these blood samples were isolated and cultured. The effects of different dose of trace elements on the proliferation and migration activity of EPCs were detected by MTT colorimetry and Transwell chamber technology. The effects of different doses of trace elements on the expression levels of Caspase-3 mRNA and Caspase-3 protein of EPCs were determined by RT-PCR and Western blot. Results: As the dose of trace elements had decreased, the optical density and the number of migrating cells of EPCs in trace elements 6 ml group(2.06±0.09, 38.71±4.59/cells×200), in trace elements 4 ml group(1.70±0.10, 29.47±3.52/cells×200), in trace elements 2 ml group(1.52±0.09, 25.47±4.62/cells×200), and in control group(1.33±0.04, 17.88±2.75/cells×200), which had decreased gradually. As the dose of trace elements had decreased, the expressions of caspase-3 mRNA and caspase-3 protein of EPCs in trace elements 6 ml group(0.217±0.05caspase-3/GAPHD, 0.212±0.03), in trace elements 4 ml group(0.537±0.08caspase-3/GAPHD, 0.427±0.04), in trace elements 2 ml group(0.669±0.09caspase-3/GAPHD, 0.617±0.05), and in control group(0.880±0.06caspase-3/GAPHD, 0.749±0.04) had increased gradually(all P<0.05). Conclusion: Trace elements may promote the proliferation and enhance the migration activity of the EPCs of pregnant women with preeclampsia and abnormal glucose and lipid metabolism, and which can down regulate the expression quantities of Caspase-3 mRNA and Caspase-3 protein of the EPCs.
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