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Abstract To explore the value of P16 autoantibody(P16 IgG) level combined with HPV detection for diagnosing high-grade squamous intraepithelial lesion(HSIL), and to evaluate the prognosis of these women. Methods: A total of 85 women with HSIL who wanted surgery treatment were selected in observation group, and 85 healthy women who wanted cervical examination were selected in control group from March 2019 to March 2020. The serum P16 IgG and HPV-DNA levels of the women in the observation group were detected before operation, and which of the women in the control group were detected during physical examination, respectively. The women in the observation group were followed up for 12 months after operation and were divided into group A(women with progression of HSIL) and group B(women with stabilization of HSIL). The values of the serum P16 IgG and HPV-DNA levels of the women in the observation group for diagnosing their HSIL and for evaluating their prognosis were analyzed. Results: The levels of preoperative serum P16 IgG(1.26±0.30) and HPV-DNA(2.12±0.69) of the women in the observation group were significantly higher than those(1.06±0.18 and 0.41±0.17) of the women in the control group. The serum P16 IgG level(1.11±0.20) and the HPV-DNA level(0.85±0.24) of the women in the observation group after operation had decreased significantly, and which were significantly higher than those(1.06±0.18 and 0.41±0.17) of the women in the control group after operation(all P<0.05). There were 28 women with progression or reoccurrence of HSIL in the observation group during the follow up for 12 months after operation. The serum P16 IgG and HPV-DNA levels of the women in group A were significantly higher than those of the women in group B. The serum P16 IgG and HPV-DNA levels of the women in group A after operation had decreased significantly, and which were significantly lower than those of the women in group B after operation(P<0.05). There was a positive correlation between the serum P16 IgG level of the women in observation group and their HPV-DNA level(r=0.784, 0.803, P<0.001). The area under curve(AUC) of the P16 IgG level and HPV-DNA level for diagnosing HSIL were 0.878 and 0.736, the best cut-off values of the P16 IgG level and HPV-DNA level for diagnosing HSIL were 1.21 and 2.11, the sensitivity of the P16 IgG level and HPV-DNA level for diagnosing HSIL were 91.3% and 81.4%, and the specificity of the P16 IgG level and HPV-DNA level for diagnosing HSIL were 78.8% and 69.6%. The AUC, the sensitivity and the specificity of the combined detection of P16 IgG and HPVDNA levels for diagnosing HSIL were 0.933, 97.7%, and 92.6%, respectively. The AUC of P16 IgG and HPV-DNA levels of the women for assessing prognosis of HSIL were 0.771 and 0.704, the best cut-off values of P16 IgG and HPV-DNA levels of the women for assessing prognosis of HSIL were 1.10 and 0.86, the sensitivity of P16 IgG and HPV-DNA levels of the women for assessing prognosis of HSIL were 86.5% and 79.4%, and the specificity of P16 IgG and HPVDNA levels of the women for assessing prognosis of HSIL were 71.7% and 66.8%. The AUC, the sensitivity and the specificity of the combined detection of P16 IgG and HPV-DNA levels for assessing prognosis of HSIL were 0.897, 91.7%, and 90.1%, respectively. Conclusion: The abnormally elevated levels of P16 IgG and HPV-DNA are related to the cervix HSIL lesions of the women, and the detections of P16 IgG and HPV levels can be used as auxiliary indicators for the early diagnosis and prognosis evaluation of the cervix HISL.
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