o analyze the effect and safety of dexmedetomidine combined anesthesia during laparoscopic hysterectomy of patients. Methods: A total of 120 patients who wanted laparoscopic hysterectomy were selected and randomly divided into two groups(60 cases in each group) from January 2019 to January 2021. The patients in the two groups were given the same induction anesthesia. The patients in the control group were given 0.5ug/kg/h sufentanil for maintaining anesthesia, and the patients in the observation group was given 0.5ug/kg/h dexmedetomidine combined anesthesia for maintaining anesthesia. The changes of hemodynamic indexes of the patients in the two groups during anesthesia were observed. The perioperative indicators and adverse reactions of the patients in the two groups were recorded. Results: The values of heart rate(HR) and mean arterial pressure(MAP) of the patients in the observation group at 5min after operation(T4) and at extubation(T6) were significantly lower than those of the patients in the control group(P<0.05). There were no significant differences in the values of HR, and MAP of the patients before anesthesia(T1), 5min after intubation(T2), 5min after pneumoperitoneum creation(T3), and 5min after pneumoperitoneum end(T5) between the two groups(P>0.05). There was no significant difference in SpO2 value of the patients at T1, T2, T3, T4, T5, and T6 between the two groups(P>0.05). There were no significant differences in the operation time(68.2±9.7min vs. 67.1±9.2min), the anesthesia time(48.8±7.1min vs. 49.6±7.5min), and the extubation time(15.2±4.6min vs. 14.9±4.3min) of the patients between the two groups(P>0.05). The Riker score of extubation agitation(2.2±0.6 points), and the incidence of nausea and vomiting(1.7%) and total adverse reactions(6.7%) of the patients in the observation group were significantly lower than those(1.1±0.3 points, 13.3%, and 23.3%) of the patients in the control group(all P<0.05). Conclusion: Dexmedetomidine combined anesthesia during laparoscopic hysterectomy has little influence on the perioperative hemodynamics of the patients, and which can effectively reduce the agitation of extubation with good anesthesia effect and safety.