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| Expression of serum long non-coding RNA-OIS1 of patients with cervical cancer and its correlation with their cervical human papillomavirus infection |
| 1. Hainan Women's and Children's Medical Center, Haikou, Hainan Province, 570216; 2. Hainan Medical College |
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Abstract To investigate the expression of serum long non-coding RNA-OIS1 (lncRNA-OIS1) of patients with cervical cancer, and to study its correlation with the cervical human papillomavirus (HPV) infection of the patients. Methods: From February 2020 to December 2021, 66 patients with cervical cancer were included in group A, 75 patients with cervical intraepithelial neoplasia were included in group B, and 72 patients with cervicitis were included in group C. The expression level of serum lncRNA-OIS1 of the patients in the three groups was detected by quantitative real-time PCR (qRT-PCR). HPV DNA detection kit and HPV E6/E7 mRNA kit were used to detect HPV positive expression and HPV E6/E7 mRNA load in cervical exfoliated cells of these patients. Spearman correlation analysis was performed to analyze the correlation between the serum lncRNA-OIS1 expression level of the patients with cervical cancer and their cervical HPV infection. Multivariate Logistic regression was performed to analyze the risk factors affecting the cervical cancer occurrence. Receiver operating characteristic (ROC) curve was performed to analyze the diagnostic value of serum lncRNA-OIS1 level for cervical cancer. Results: The expression levels of lncrNA-OIS1 of the patients in group C, in group B, and in group A were 1.01±0.17, 0.82±0.16, and 0.69±0.18, respectively, which had decreased gradually. The positive expression rates of HPV of the patients in group C, in group B, and in group A were 26.4%, 45.3%, and 100.0%, respectively, which had increased gradually, and the high load rate of HPV E6/E7 mRNA of the patients in group C, in group B, and in group A were 19.4%, 37.3%, 100.0%, respectively, which had increased gradually (all P<0.05). Spearman correlation analysis showed that the expression level of lncrNA-OIS1 of the patients with cervical cancer was negatively correlated with their HPV positive expression and their HPV E6/E7 mRNA load (r=-0.526, -0.489, all P=0.000). The low expression of lncrNA-OIS1, the positive HPV, and the high HPV E6/E7 mRNA load of the patients were the risk factors of their cervical cancer (P<0.05). The area under ROC curve of serum lncrNA-OIS1 level of the patients for diagnosing their cervical cancer was 0.892. Conclusion: The expression level of serum lncRNA-OIS1 of the patients with cervical cancer decreases, which is closely related to their cervical HPV infection. It has certain value for diagnosing cervical cancer and can be used as a biomarker for predicting cervical cancer.
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