To explore the effect and mechanism of vascular endothelial growth factorA (VEGF-A) inhibitor intervening model rats with endometriosis. Methods: 35 SD healthy female rats were selected and divided in 2 groups, which included 5 rats in the normal group and 30 rats were in the endometriosis model group. And the 30 rats in the endometriosis model group were randomly divided into group A (10 rats were given saline), group B (10 rats were given danazol), and group C (10 rats were given VEGF-A inhibitor). The levels of estradiol (E2), progesterone (P), follicle stimulating hormone (FSH), luteinizing hormone (LH), C-reactive protein (CRP), interleukin-8 (IL-8), interleukin-6 (IL-6) of rates in the four groups were detected, and the levels of Hypoxia inducible factor-1α (HIF-1α) and VEGF, and the expression of Bcl-2, Bax, caspase-3 proteins of rates in the four groups were detected by Western blot. Results: The levels of E2, P, FSH, LH, CRP, IL-8 and IL-6, and the relative expression of VEGF, HIF-1α, Bcl-2 and Caspase-3 of rats in group C were significant lower than those in group A and B, but the relative expression level of Bax of rats in group C was significant higher (P<0.05). Conclusion: VEGF-A inhibitors used for intervening the model rats with endometriosis can regulate their sex hormones level, inhibit the inflammation and growth of heterotopic endometrium tissue, and promote apoptosis of ectopic endometrium tissue.

2020 Vol. 28　(7):　977-980 [Abstract]（ 475 ） HTML (0 KB)  PDF  (0 KB)  ( 7 )

To explore and evaluate the effect of reproductive health education interventions of undergraduates from four colleges in Guangzhou. Methods: A random stratified cluster sampling method was used to select 750 undergraduates from four colleges in Guangzhou for reproductive health education interventions. The effect was evaluated and analyzed by questioner before and after intervention. Results: Before and after intervention, condoms accounted for the highest proportion of contraception used of undergraduates in both knew and considered suitable contraceptives for teenagers. After the intervention, the awareness rate of contraceptives of undergraduates had increased significantly, and the proportion of effective suitable contraceptives for teenagers by undergraduates chosen (included compound oral contraceptives, subcutaneous implants, and intrauterine devices) had increased significantly. And the proportion of low-efficiency contraceptives and long-acting irreversible contraceptive (included safety period, in vitro sperm expulsion, external contraceptive, and male/female sterilization) had decreased significantly (P<0.001). The correct rate of answering the five common contraceptive questions had increased significantly (P<0.01). 99.7% of undergraduates had thought that it was necessary to carry out lectures on reproductive health education in universities. Conclusion: The reproductive health education in universities can increase the contraceptive knowledge of undergraduates significantly. The intervention effect is significant and feasible, so the needs of reproductive health education of undergraduates should be paid more attention to.

2020 Vol. 28　(7):　986-989 [Abstract]（ 401 ） HTML (0 KB)  PDF  (0 KB)  ( 7 )

 To study the change of concentration of cupric ion in cervical mucus before and after copper-bearing intrauterine device (IUD) removal. Methods: In this prospective cohort study, women with copper-bearing IUD inserted for least 3 month were included in study group, and women with condoms used only were included in control group. The difference of copper ion concentration in cervical mucus before and 1 month and 6 months after the removal of IUD were measured and compared between the two groups. As well as the copper ion concentration in cervical mucus of women with IUD used for different time or IUD with different copper surface area in the study group was also measured and analyzed. Results: The concentration of copper ions in cervical mucus of women in the study group before IUD removal was 2.1 (1.4, 4.9) μg/g, which was significant higher than that ［0.2 (0.1, 0.3) μg/g］ of women in the control group (Z=-10.4, P< 0.0001). The copper ion concentration of women in the study group in 1 month and 6 months after IUD removal was 0.2 (0.1, 0.3) μg/g and 0.2 (0.1, 0.3) μg/g, which were similar to those of women in the control group (Z=-0.72, P=0.47; Z=-0.86, P=0.38). There was no significant difference in the concentration of copper ions in cervical mucus before IUD removal among women with different time of IUD used (χ2=3.35, P=0.34). The concentration of copper ions in the cervical mucus of women with copper surface area ≥300 mm2 before IUD removal was 2.2 (1.0, 15.3) μg/g, which had no significant different from that ［2.1 (1.4, 3.9) μg/g］ of women with copper surface area <300 mm2 (Z=0.29, P=0.77). Conclusion: The concentration of copper ions in cervical mucus of women will return to the normal level in 1 month after IUD removal. The copper ion concentration in cervical mucus of women is not related to the duration of IUD used and the copper surface area of copper-bearing IUD.

2020 Vol. 28　(7):　994-997 [Abstract]（ 428 ） HTML (0 KB)  PDF  (0 KB)  ( 8 )

 To analyze the effect of combined oral contraceptives (COC) on postoperative complications and ovarian function of women after induced abortion. Methods: 150 women after abortion during the first trimester of pregnancy were divided into group A (50 women were given Marvelon), group B (50 women were given Diane-35), and group C (50 women were not given any controceptive) according to their controceptive schedule from January 2018 to June 2018. The bleeding volume after abortion, endometrium situation, complications, and the serological indexes of ovarian function in the early follicular phase of women were compared among these groups. Result: The days and amount of vaginal bleeding of women in group A were the lowest, but those of women in group C were the highest. The rate of menstrual punctual recovery of women in group A was the highest, but that of women in group C was lowest (all P<0.05). The incidence of postoperative complication of women in group A and B were 0.0% and 0.0%, which was significant lower than that (14.0%) of women in group C (P<0.05). The levels of serum LH, E2, AMH, and INH-B, and the improvement of PI value and endometrial blood flow signal of women in group A and B were significant higher than those of women in group C, but the levels of FSH, FN, and PSV were significant lower than those of women in group C (all P<0.05). There were no significant different in the levels of serum LH, E2, AMH, and INH-B, and the improvements of PI value and endometrial blood flow signal, and the levels of FSH, FN, and PSV of women between group A and B. Conclusion: The short acting COC can effectively reduce the postoperative complications and improve ovarian function of women after abortion. And Marvelon and Diane-35 have the same safety and efficacy.

2020 Vol. 28　(7):　1002-1005 [Abstract]（ 445 ） HTML (0 KB)  PDF  (0 KB)  ( 10 )

To investigate the effect of laparoscopy and hysteroscopy combined with gonadotropin releasing hormone analogue (GnRH a) for treating infertility women with endometriosis on their levels of serum sex hormones, 5 lipoxygenase (5 LOX), and lipoxin A4 (LX A4). Methods: 55 infertility women with endometriosis who had undergone laparoscopy combined with hysteroscopy were enrolled in this study, and were divided into two groups according to willingness of the women from June 2017 to December 2018. 25 women in the control group had received medroxyprogesterone acetate tablets, while 30 women in the observation group had received GnRH a treatment. The clinical efficacy, the levels of serum sex hormones, 5 LOX, and LX A4, and visual analogue scale (VAS) scores of women were compared between the two groups. Results: The total recurrence rate of women in the observation group within 1 year after surgery was 3.3%, which was significant lower than that (28.0%) of women in the control group. The cumulative clinical pregnancy rate within 1 year and 2 years after surgery were 63.3% and 76.7%, which were significant higher than those (28.0% and 48.0%) of women in the control group (all P<0.05). The postoperative fertility rate of women in the two groups were 6.03% and 2.26%, and the odd ratio of them was 2.67. After operation, the levels of E2, LH and FSH of women in both groups had decreased significantly, which of women in the observation group were significant lower than those of women in the control group, meanwhile, the levels of LXA4 and 5 LOX of women in both groups had increased significantly, which of women in the observation group were significant higher than those of women in the control group (P<0.05). The scores of sexual intercourse pain, chronic pelvic pain, and dysmenorrhea of women in the observation group were significant lower than those of women in the control group (P<0.05). Conclusion: After operation, GnRH a can effectively increase the expression levels of 5 LOX and LX A4 of infertility women with endometriosis, and can regulate their level of hormones, thereby GnRH a can reduce the postoperative recurrence rate and increase the pregnancy rate.

2020 Vol. 28　(7):　1010-1013 [Abstract]（ 485 ） HTML (0 KB)  PDF  (0 KB)  ( 0 )

To analyze the correlation between the levels of inhibin B (INH-B), leptin (Leptin), and anti-Mullerian hormone (AMH) of patients with polycystic ovary syndrome (PCOS) and their sex hormones levels. Methods: 120 patients with PCOS were selected in study group from January 2018 to January 2019. Another 120 healthy women who underwent physical examination were selected in control group during the same period. The levels of INH-B, Leptin, AMH and sex hormones of the women in both groups were detected, which’s correlation with the obesity and the disease classification of patients with PCOS was analyzed. Pearson correlation analysis was used to analyze the relationship between the levels of INH-B, Leptin, AMH, INH-B, Leptin, AMH and sex hormones levels. Results: The serum levels of INH-B, Leptin, AMH, testosterone(T) and luteinizing hormone (LH) of women in the study group were significant higher than those of women in the control group, but the level of FSH was significant lower than that of women in the control group (P<0.05). In the study group, the levels of Leptin, AMH, and T of women were positively correlated with their obesity, but the INH-B and LH levels were negatively correlated with their obesity (P<0.05), and the FSH level was not correlated with obesity (P>0.05). The levels of serum INH-B, Leptin, AMH, T and LH were positively correlated with the severity of the disease (P<0.05). The INH-B level was positively correlated with the levelsof Leptin and AMH (r=0.328, 0.236, P=0.016, 0.028), but the Leptin level was positively correlated with the AMH level (r=0.685, P=0.002). The levels of INH-B, Leptin, and AMH were positively correlated with the levels of T and LH, but were negatively correlated with the FSH level (P<0.05). Conclusion: INH-B, Leptin and AMH of patients with PCOS are involved in the occurrence and development of PCOS, which are related to abnormal sex hormones levels, so they maybe used as predictors for detecting PCOS.

2020 Vol. 28　(7):　1018-1021 [Abstract]（ 436 ） HTML (0 KB)  PDF  (0 KB)  ( 0 )

To investigate the expression features of serum high-density lipoprotein (HDL) of pregnant women with gestational diabetes mellitus (GDM) and pregnancy-induced hypertension syndrome, and to study its predictive value for pregnancy outcomes. Methods: In this study, 95 pregnant women with GDM and pregnancy-induced hypertension syndrome were selected in observation group from 2014 to 2016, and 100 healthy pregnant women were selected in control group. The fasting blood glucose, OGTT, high-density lipoprotein (HDL) level, and blood pressure of the women in the two groups were measured. And the relationship between serum HDL expression of the women in the observation group and their pregnancy outcomes was analyzed. Results: The fasting glucose level of women in the 28th gestational weeks in the observation group was 5.64±0.79 mmol/L, which was significant higher than that (4.46±0.50 mmol/L) of women in the control group. The values of systolic and diastolic blood pressure of women in the observation group were 156.68±13.94 mmHg and 7.66±9.07 mmHg, which were significant higher than those of women in the control group. The serum HDL level of women in the observation group was 1.36±0.29 mmol/L, which was significant lower than that (1.80±0.33 mmol/L) of women in the control group. The blood glucose level 1 hour and 2 hours after dinner, and the levels of low density lipoprotein (LDL), triglyceride and cholesterol women in the observation group were significant higher than those of women in the control group. The proportion of vaginal delivery of women with low HDL level was 51%, which was significant lower than that of women with normal HDL level. The incidence of postpartum hemorrhage of women with lowr HDL level 8.1%, which was significant higher than that of women with normal HDL level (P<0.05). There were no significant different in the incidences of vaginal midwifery, cesarean section, neonatal asphyxia, and stillbirth between the women with low HDL level and the women with normal HDL level. The multivariate regression analysis showed that the abnormal HDL level decreased, fasting blood glucose level, LDL level and abnormal systolic blood pressure increased of women were associated with their adverse pregnancy outcomes. Conclusion: The HDL level of pregnant women with GDM and pregnancy-induced hypertension syndrome is significantly has decreased, which is related to the the proportion of normal vaginal delivery and postpartum hemorrhage, so it is should be paid more attention to in the clinical practice.

2020 Vol. 28　(7):　1026-1028 [Abstract]（ 486 ） HTML (0 KB)  PDF  (0 KB)  ( 7 )

To investigate the expression of 25-hydroxyvitamin D［25(OH)D］, mean platelet volume (MPV), and homocysteine (Hcy) of women with hypertensive disorder complicating pregnancy, and to study their relationship with the disease severity. Methods: 80 women with hypertensive disorder complicating pregnancy were selected in study group from June 2017 to June 2019. 80 healthy pregnant women were selectedin control group during the same period. The blood levels of of 25-(OH) D, MPV and Hcy of women in the two groups were detected. The influencing factors of the occurense and the disease severity of hypertensive disorder were analyzed. Results: The level of blood 25-(OH)D of women in the study group was significant lower than that of women in the control group, but the levels of MPV and Hcy of women in the study group were significant higher (P<0.05). In the study group, the level of blood 25-(OH) D of women had gradually decreased with the severity of the disease, but the levels of MPV and Hcy had gradually increased (P<0.05). Logistic regression analysis showed that the levels of blood 25-(OH) D, MPV and Hcy were the risk factors for the development and the severity of hypertensive disorder complicating pregnancy. Spearman correlation analysis revealed the expression of blood 25-(OH) D was negatively correlated with the severity of hypertensive disorder complicating pregnancy (r1=-0.762), but the expression of MPV and Hcy was positively correlated with the severity of hypertensive disorder complicating pregnancy (r2=0.506, r3=0.794, P<0.05). Conclusion: In pregnant women with hypertensive disorder complicating pregnancy, the level of blood 25 (OH) D is low, and the levels of MPV and Hcy are high, which are closely related to their disease severity, so they can provide evidence for clinical diagnosis and treatment.

2020 Vol. 28　(7):　1033-1036 [Abstract]（ 410 ） HTML (0 KB)  PDF  (0 KB)  ( 0 )

To investigate the influence of preventive iron supplementation during pregnancy for treating pregnant women on their erythropoietin level and pregnancy outcomes. Methods: 100 pregnant women in the 11-12th gestational week were selected and were divided into study group and control group. The women in the study group were given preventive iron supplementation therapy from the 12th gestational week and the women in the control group were not given iron supplementation therapy. The clinical efficacy was compared between the two groups. Results: The incidences of iron deficiency (ID) and iron deficiency anemia (IDA) of women in the 28th and the 34th gestational week in the study group were 26.0%, 10.0%, 44.0%, and 16.0%, respectively, which were significant lower than those (60.0%, 36.0%, 90.0%, 44.0%, respectively) of women in the control group. Compared with those of women in the control group, the levels of erythropoietin, ferritin, hemoglobin, red blood cell volume, and related immune indexes of women in the study group had improved significantly. The rate of pregnancy complications and adverse pregnancy outcomes of women in the study group was 14.0%, which were significant lower than that (32.0%) of women in the control group (all P<0.05).Conclusion: The preventive iron supplementation therapy during pregnancy can prevent IDA of pregnant women effectively, and can significantly improve their immune function and decrease the incidences of pregnancy complications and adverse pregnancy outcomes.

2020 Vol. 28　(7):　1041-1043 [Abstract]（ 390 ） HTML (0 KB)  PDF  (0 KB)  ( 37 )

To investigate the influence of vitamin D combined with individualized exercise and dietary intervention on islet β-cells and pregnancy outcomes of pregnant women with gestational diabetes mellitus (GDM). Methods: From January 2016 to December 2017, 86 pregnant women with GDM were included in this prospective study, and they were divided into control group and observation group (43 cases in each group) by random number table method. All women in the two groups were given insulin aspart subcutaneous injection, and the women in the observation group were given oral vitamin D3, individualized exercise and dietary intervention additionally. The related parameters of blood sugar, the inflammatory factor levels, the ischemia and hypoxia related indicators, the levels of serum homocysteine (HCY) and serum cystatin C (Cys-C), the indicators related to islet beta cells, and the rates of adverse pregnancy and fetal outcomes were compared between the two groups. Results: There were no significant different in related blood glucose indicators, inflammatory factor levels, ischemia and hypoxia related indicators, levels of HCY and Cys-C, and related indicators of islet-βcell before treatment between the two groups (P>0.05). After treatment, the levels of FPG, 2hPG, HbAlc, hs-CRP, TNF-α, HIF-1α, ET-1, Bax, Caspase-3, HCY, Cys-C, FINS, and HOMA-IR of women in the observation group were significant lower than those of women in the control group, but the levels of NO, HOMA-β and ISI were significant higher than those of women in the control group (P<0.05). And the rates of adverse pregnancy outcomes and adverse fetal outcomes of the observation group were 23.3% and 23.3%, which were significant lower than those (58.1% and 44.2%) of the control group (P<0.05). Conclusion: Vitamin D combined with individualized exercise and dietary intervention can effectively improve the blood glucose control effect of pregnant women with GDM, can decrease their level of serum inflammatory factors, can relieve their placental ischemia and hypoxia, can enhance their islet β cell function, and can improve their pregnancy and fetal outcomes.

2020 Vol. 28　(7):　1048-1052 [Abstract]（ 392 ） HTML (0 KB)  PDF  (0 KB)  ( 22 )

To investigate the correlation between Sigma-1 receptor and degenerative of infertile patients with low ovarian reserve. Methods: 80 infertile women were enrolled in the study from January 2016 to December 2018. According to the ovarian reserve function, 47 patients with low ovarian reserve were included in observation group, and 33 patients with normal ovarian reserve were included in control group. The expression levels of Sigma-1 receptor protein in plasma, follicular fluid, and granular cell were compared between the two groups and the correlation of them were analyzed. Results: The levels of basal follicular generation hormone (FSH), age of patients in the observation group were significant higher than those of patients in the control group, but the levels of anti-Mulller's hormone (AMH), basal antral follicular count (AFC), the acquired eggs, available embryos, and expression of plasma Sigma-1 receptor protein, follicular fluid, and granular cell of patients in the observation group were significant lower than those of patients in the control group (P<0.05). In the observation group, the expression of Sigma-1 receptor protein in granular cells and plasmaand follicular fluid was negative correlated with basal FSH level and age of patients, and was positive correlated with AMH level, AFC, number of eggs obtained, and number of available embryos (P<0.05). Conclusion: Sigma-1 receptor maybe participate in the regulation of ovarian reserve function, which’s low expression is associated with the low ovarian reserve, so it is suggests that Sigma-1 receptor may be used as a potential clinical marker for predicting ovarian reserve.

2020 Vol. 28　(7):　1056-1059 [Abstract]（ 400 ） HTML (0 KB)  PDF  (0 KB)  ( 23 )

To analyze the value of E6/E7 mRNA of HPV detection for predicting disease progression of patients with atypical squamous cells of undetermined significanc (ASCUS) in 24 months, and to provide evidence for shunting management of patients. Methods: 476 patients with ASCUS by TCT were selected as the study objects from January 2016 to December 2017. The difference of the positive detection rates of patients with different cervical biopsy results were compared between E6/E7 mRNA of HPV detection and HPV DNA detection, and the efficacy of E6/E7 mRNA of HPV detection and HPV DNA detection for predicting patients with cervical biopsy pathology≥HSIL in 12 and 24 months followed up was analyzed. Results: The positive rate of HPV DNA detection for patients with chronic inflammation and LSIL was significant higher than that of E6/E7 mRNA of HPV detection (P<0.05), and there was no statistical significant difference in the positive rate of HSIL and cervical cancer between HPV DNA detection and E6/E7 mRNA of HPV detection (P>0.05). The specificity and positive predictive value of E6/E7 mRNA of HPV detection for patients with HSIL or above lesions were 40.5% and 23.7%, which were significant higher than those (19.0% and 18.4%) of HPV DNA detection (P<0.001). There were no significant difference in the sensitivity and the negative predictive value between E6/E7 mRNA of HPV detection and HPV DNA detection (P>0.05). Both the primary E6/E7 mRNA of HPV detection and HPV DNA detection had certain predictive efficacy for the patients with cervical biopsy pathology≥HSIL in 12 months and 24 months followed up (P<0.001), the predictive efficacy for the patients in the 12th month after diagnosed was significant better than that in the 24th month, and the predictive efficacy by E6/E7 mRNA of HPV detection E6/E7 mRNA was superior to that by HPV DNA detection. Conclusion: The E6/E7 mRNA of HPV detection can better distinguish the high-grade lesions of patients with ASCUS, which’s prediction value for patients with disease progression within 12 months after diagnosed is superior to that by HPV DNA detection.

2020 Vol. 28　(7):　1064-1067 [Abstract]（ 400 ） HTML (0 KB)  PDF  (0 KB)  ( 28 )

To analyze the risk factors of early pregnancy loss of pregnant women. Methods: The clinical data of 680 pregnant women from March to February 2018 were collected, which included 80 women with early pregnancy loss in 13 gestational weeks in study group and 600 normal pregnant women in control group. The incidence of early pregnancy loss was compared among pregnant women with different characteristics, and the risk factors of pregnancy loss were analyzed. Results: Among 680 pregnant women, the incidence of early pregnancy loss was 11.8%. The rates of previous abortion history, congenital uterine malformation, acquired coagulopathy, early viral infection, polycystic ovary syndrome of women with early pregnancy loss were significant higher than those of normal pregnant women (P<0.05). There was no significant different in the incidence of pregnancy loss among women with different age (P>0.05). When the pregnancy loss was used as the dependent variable, and previous abortion history, congenital uterine malformation, acquired coagulopathy, early viral infection, polycystic ovary syndrome from the single factor analysis results were as the independent variable, the multivariate logistic regression analysis showed that congenital uterine malformation and acquired coagulopathy were risk factors for pregnancy loss (OR=4.987, 4.683). Conclusion: There is a certain incidence of early pregnancy loss of pregnant women with different age. The congenital abnormalities of the uterine cavity and history of acquired diseases maybe influent the early pregnancy loss.

2020 Vol. 28　(7):　1071-1073 [Abstract]（ 791 ） HTML (0 KB)  PDF  (0 KB)  ( 26 )

To explore the changes of the levels of serum vascular endothelial growth factor (VEGF), high homocysteine (Hcy), and hypoxia inducible factor-1α(HIF-1α) of patients with missed abortion, and to analyze the risk factors of missed abortion. Methods: 42 patients with missed abortion were included in observation group, and 42 normal pregnant women who wanted induced abortion were included in control group. The levels of VEGF, Hcy and HIF-1α of patients were compared between the two groups. And single factor analysis and logistic regression analysis were used for statistical analysis of the clinical data of patients. Results: The levels of VEGF and HIF-1α of patients in the observation group were significant lower than those of patients in the control group (P<0.05). The results of variance analysis showed that there was significant different in proportions of the older women, smoking history, radiation overdose accepted, folic acid supplementation, knowledge of reproduction, or abnormal thyroid function of patients between the two groups. Logistic regression analysis showed that older women, smoking history, radiation overdose accepted, thyroid dysfunction and Hcy level were the risk factors for missed abortion, while folic acid supplementation, high level of reproductive knowledge, and levels of VEGF and HIF-1α were the protective factors for missed abortion (all P<0.05). Conclusion: The levels of serum VEGF, Hcy and HIF-1α, older women, smoking history, radiation overdose accepted, folic acid supplementation, knowledge level of reproduction, and abnormal thyroid function were all closely related to missed abortion.

2020 Vol. 28　(7):　1078-1081 [Abstract]（ 351 ） HTML (0 KB)  PDF  (0 KB)  ( 29 )

To explore the value of the levels of serum interacting protein 1 (LRRFIP1), placental growth factor (PLGF), and soluble Fms-like tyrosine kinase-1 (sFlt-1) for diagnosing and prognosis evaluation of elderly women with pregnancy-induced hypertension. Methods: 61 elderly women with pregnancy-induced hypertension were selected in observation group, which included 26 women with mild pregnancy-induced hypertension in group A, 25 women with mild preeclampsia in group B, and 10 women with severe preeclampsia in group C. Another 50 normal pregnant women were selected in control group. The levels of serum LRRFIP1, PLGF and sFlt-1 of all included women were detected, and the ratio of sFlt-1 to PLGF was calculated. All women were followed up until the termination of pregnancy. The differences of the levels of serum LRRFIP1, sFlt-1, and PLGF, and sFlt-1/PLGF value of women with different illness degree and with different pregnancy outcomes were observed. ROC analysis was used to explore the value of the levels of serum LRRFIP1, sFlt-1, and PLGF, and sFlt-1/PLGF value for diagnosing pregnancy-induced hypertension of elderly pregnant women and predicting their prognosis. Results: The serum PLGF level of women in the observation group was significant lower than that of women in the control group (P<0.05). The PLGF level had decreased with the aggravation of pregnancy-induced hypertension, but the levels of serum LRRFIP1 and sFlt-1, and PLG, and sFlt-1/PLGF value had increased with the aggravation of pregnancy-induced hypertension. The PLGF level of women with adverse pregnancy outcomes was significant lower than that of women with normal pregnancy outcomes, but the levels of serum LRRFIP1 and sFlt-1, and PLG, and sFlt-1/PLGF value of women with adverse pregnancy outcomes was significant higher (all P<0.05). The area under curve (AUC) of sFlt-1/PLGF ratio for diagnosing pregnancy-induced hypertension and predicting adverse pregnancy outcomes were 0.922 and 0.937, which were significant higher than those (0.901 and 0.891) of the levels of sFlt-1, PLGF, and LRRFIP1. Conclusion: The serum LRRFIP1 level and sFlt-1/PLGF value of elderly women with pregnancy-induced hypertension has significantly increased, and they are closely related to the severity and prognosis of the disease, which can be used as the auxiliary indexes for diagnosing and predicting pregnancy outcomes of elderly women with pregnancy-induced hypertension.

2020 Vol. 28　(7):　1087-1090 [Abstract]（ 489 ） HTML (0 KB)  PDF  (0 KB)  ( 0 )

To analyze the expression levels of elastase (NE) and miR-551b in prostatitis massage fluid (EPS) of infertility men with prostatitis, and to study their diagnostic value. Methods: Between July 2017 and May 2019, 60 infertility men with prostatitis were selected in observation group, and 60 healthy male volunteers were selected in control group during the same period. The expressions levels of NE and miR-551b in EPS of man were compared between the two groups. The correlation between NE level and miR-551b level was analyzed. And the diagnostic efficacy and the area under ROC curve (AUC) of the levels of NE and miR-551b of infertility men with prostatitis were also analyzed. Results: The NE expression level of men in the observation group was 1472.25 (812.23, 1735.65), which was significant higher than that ［156.38 (76.35, 226.52)］ of men in the control group. The expression level of mir-551b of men in the observation group was 5.13 (2.34, 13.47), which was significant lower than that ［22.31 (8.64, 42.23)］ of men in the control group (P<0.05). Spearman rank correlation analysis showed that NE level was negative correlated with the miR-551b level of men in the observation group (r=-0.874, P<0.05). The sensitivity, the specificity, and the accuracy of the levels of NE and miR-551b for combined diagnosing prostatitis of infertility men were 79.2%, 81.5%, and 84.3%, respectively, which were all significant higher than those of diagnosing by NE level or miR-551b level only. The AUC of NE and miR-551b for combined diagnosing infertility of man with prostatitis was also significant higher than that of diagnosing by NE level or miR-551b level only (P<0.05). Conclusion: The combined of NE and miR-551b levels in EPS has better value for diagnosing infertility of men with prostatitis, which is expected to be molecular markers for diagnosing infertility of men with prostatitis.

2020 Vol. 28　(7):　1095-1097 [Abstract]（ 361 ） HTML (0 KB)  PDF  (0 KB)  ( 7 )

 To investigate the relationship between endometriosis syndrome and endometrial polyps of infertility patients, and to study the endometrial hyperplasia situation. Methods: Infertility patients were selected and divided into study group (68 patients with endometriosis) and control group (42 patients without endometriosis) according to the results by B-ultrasound examination from January 2015 to March 2018. The incidence of endometrial polyps, pregnancy rate within 18 months after treatment, and pathological result of endometrial polyps of patients in the two groups were analyzed. Spearman correlation coefficient analysis was used to analyze the correlation between endometriosis of infertile patients and the occurrance of endometrial polyps. Results: The incidence of endometrial polyp of the study group was 75.0% (51/68), which was significant higher than that (23/42, 54.8%) of the control group (P<0.05). The proportion of patients with polyps in the study group was 75.0% (51/68), which was significant higher than that (23/42, 54.8%) of patients in the control group, and the proportion of patients with normal endometrium in the study group was 5.9% (4/68), which was significant lower than that (14/42, 33.3%) of patients in the control group (P<0.05). Correlation analysis showed that endometriosis syndrome rate was positively correlated with the development of endometrial polyps of infertile patients (P<0.05). In the study group, the total pregnancy rate within 18 months after treatment of patients with endometrial polyp was 80.4% (41/51), which was significant higher than that (10/17, 58.8%) of patients without endometrial polyp (P<0.05). In the control group, the total pregnancy rate within 18 months after treatment of patients with endometrial polyp was 69.6% (16/23), which had no significant different from that (14/19, 73.7%) of patients without endometrial polyp (P>0.05). Conclusion: Endometriosis of infertility patients is proportional to the incidence of endometrial polyps, which suggested that clinical diagnosis and treatment endometriosis should be paid more attention endometrial polyp.

2020 Vol. 28　(7):　1101-1103 [Abstract]（ 351 ） HTML (0 KB)  PDF  (0 KB)  ( 7 )

 To investigate the effect different treatments of cervical lesions of pregnant women on their pregnancy outcomes and delivery mode. Methods: The clinical data of 870 pregnant women after treatment of cervical lesions from March 2012 to March 2018 were retrospectively analyzed. According to the different treatment of cervical lesions, these women were divided into Group A (207 women had received the loop electrosurgical excision procedure (LEEP), group B (105 women had received the cold knife conization (CKC), group C (302 women had received intensity focused ultrasound (IFU), and group D (256 women had received physical laser). Meanwhile, 82 healthy pregnant women were randomly selected in control group during the same period. The delivery mode, pregnancy outcomes, rate of complications during delivery, time of the total stage of labor, the cervical length in 22-24 gestational weeks, and the degree of cervical stenosis of women, and neonatal weight, length, Apgar score were compared among these groups. Results: The cesarean section rate of women in the control group, group D, group C, group A, and group B had increased in turn (P<0.05). The incidences of premature delivery and premature rupture of membranes of women in group A, B, C, and D were significant  higher than those of women in the control group (P<0.05). The incidence of cervical laceration, uterine rupture, postpartum hemorrhage, or postpartum infection of women in group B was the highest (P<0.05). There were significant different in the neonatal weight, length, and Apgar score among these groups (P<0.05). The total stage of labor time and cervical stenosis degree of women in the control group, group D, group C, group A, and group B had increased in turn (P<0.05). The cervical length of women in 22-24 gestational weeks in the control group, group D, group C, group A, and group B had shorted in turn (P<0.05). Conclusion: The treatment of the cervical lesions can maybe increase the rate of cesarean section, premature delivery, premature rupture of membranes, and infection of women after pregnancy, which suggests anti-infective treatment should be strengthened during treatment of the cervical lesions can, and the appropriate delivery mode should be given according to the situation of pregnant women for decreasing their cesarean section rate.

2020 Vol. 28　(7):　1108-1113 [Abstract]（ 502 ） HTML (0 KB)  PDF  (0 KB)  ( 7 )

To explore the clinical significance of the cervical length (CVL) measured by ultrasound on the induction time, pregnancy outcomes, and delivery mode before labor induction in term pregnancy of pregnant women with scarred uterus. Methods: 120 scarred uterus pregnant women without sign in labor who wanted labor induction during the 3940th gestational weeks were selected and were given CVL measured by ultrasound and cervical Bishop score evaluated. The women with CVL≤2.8cm were in group A, and the women with CVL＞2.8cm were in group B. And the women with Bishop score >6 points were given routine oxytocin induced labor, and the women with Bishop score ≤6 points were given promoted cervical ripening by Foley catheter balloon before oxytocin induced labor. The effect of induced labor and delivery mode of all included women were observed. Results: The cervical Bishop score, and the success rate of induced labor of women in group A was 6.03±1.05 points and 86.8%, which were significant higher than those (4.07±1.53 points and 62.2%) of women in group B, but the time from induction to delivery and the rate of conversion to cesarean section of women in group A were 20.4±9.5 h and 19.7%, which were significant lower. The rate of near to give birth within 48 hours of women with Bishop score >6 points in group A was 85.2%, which was significant higher than that (53.3%) of women in group B. Conclusion: The cervical length measured by ultrasonic score of pregnant women with scarred uterus in term pregnancy before labor induction has some certain clinical significance for guiding the induction time, induction outcomes, and delivery mode, which combined with cervical Bishop for guiding vaginal delivery is better.

2020 Vol. 28　(7):　1118-1120 [Abstract]（ 421 ） HTML (0 KB)  PDF  (0 KB)  ( 8 )

To investigate the correlation between the expression of HtrA4 in placenta tissue of women with severe preeclampsia and their perinatal outcomes. Methods: 69 pregnant women with severe preeclampsia were selected in study group and 50 normal pregnant women were selected in control group. The experience of HtrA4 in peripheral blood and placenta tissue of all women were detected. The maternal pregnancy outcomes and the experience differences of HtrA4 in placenta tissue of women in the two groups were analyzed. Pearson correlation coefficient was used to describing the correlation between HtrA4 expression in placenta tissue and neonatal birth weight, umbilical hemodynamic parameters, such as the ratio of fetal umbilical artery systolic pressure to diastolic pressure (S/D), pulsation index (PI), resistance index (RI). Results: The expressions of HtrA4 in the serum and placenta of women with severe eclampsia group were significant higher than those of women in the control group (P<0.05). The expressions of HtrA4 in the serum and placental tissues of women with adverse pregnancy outcomes and poor perinatal outcomes were significant higher than those of normal pregnancy women (P<0.05). There was a negative correlation between HtrA4 expression in placental tissue and neonatal birth weight (r=-0.478, P<0.05). There was a positive correlation between HtrA4 expression in placental tissue and S/D value, RI and PI (r=0.476, 0.540, 0.650, P<0.05). Conclusion: The expression of HtrA4 in placenta tissue of women with severe preeclampsia has up-regulate significantly, which is closely related to pregnancy outcomes and perinatal outcomes.

2020 Vol. 28　(7):　1125-1128 [Abstract]（ 370 ） HTML (0 KB)  PDF  (0 KB)  ( 9 )

To analyze the influence of multi-link feedback education mode of health care cooperation on the contraceptive knowledge level, contraceptive implement rate, and satisfaction of women with induced abortion. Methods: 240 women with induced abortion were randomly divided into control group and study group. The women in the study group were given multi-link feedback education mode of health care cooperation, and the women in the control group were given routine health education mode. The contraceptive knowledge level, contraceptive implement rate, and satisfaction degree of women with induced abortion before and after intervention were compared between the two groups. Results: The score of contraceptive knowledge and the immediate implement rate of long-acting contraception of women in the study group were 80.9±6.5 and 30.0%, which were significant higher than those (69.9±4.0 and 18.4%) of women in the control group (P<0.05). The overall satisfaction of health education of women in the study was 94.2%, which was significant higher. Conclusion: The application of multi-link feedback mode of health care cooperation for women in PAC service can significantly improve the service effects, improve their contraceptive knowledge level and immediate implement rate of high effective and long-acting contraception, and enhance their degree of satisfaction, which is worthy of clinical application.

2020 Vol. 28　(7):　1132-1135 [Abstract]（ 522 ） HTML (0 KB)  PDF  (0 KB)  ( 5 )

Vaginal rings is one of the sustained and controlled release drug delivery systems. With the continuous technological development of vaginal rings, they have been accepted widely, especially in reproductive health field. At an early stage, they were mainly used for contraception and hormone replacement therapy. In recent years, they have been generally applied in the field of reproductive health, such as assisted reproductive techniques, anti-HIV infections, and the treatment of endometriosis. Furthermore, vaginal rings make it possible to deliver two or more drugs simultaneously for achieving the multipurpose therapy, such as HIV prevention combined with contraception.

2020 Vol. 28　(7):　1143-1148 [Abstract]（ 502 ） HTML (0 KB)  PDF  (0 KB)  ( 9 )