To explore the relationship between the levels of trace elements in seminal plasma and the sperm quality of male infertile patients. Methods: 100 infertile men were selected in the infertile group, and 200 healthy men with the same age were selected in the control group. The levels of zinc, copper, iron, magnesium and calcium in the seminal plasma, and the semen routine parameters of men in the two groups were detected. Results: The levels of zinc, iron and calcium in the seminal plasma of men were 1409.3±114.0, 4.11±0.84, and 5.18±1.13, respectively, which were significant lower than those (1568.6±163.9, 4.73±0.95, and 5.80±1.20, respectively) of men in the control group, but the level of copper in the seminal plasma of men in the infertile group was 4.75±0.80， which was significant lower than that (3.32±0.66) of men in the control group (P<0.05). The sperm concentration, the proportion of sperm forward movement, and the sperm motility of men in the infertile group were 114.8±45.8, 49.4±7.8, and 69.4±8.8, respectively, which were significant lower than those (136.2±48.1, 58.6±8.2, and 78.5±10.4, respectively) of men in the control group (P<0.05). In the infertile group, the levels of zinc, iron and calcium in the seminal plasma of men were significantly positive correlation with the sperm density and concentration, sperm forward movement ratio, or sperm motility, but the level of copper were significantly negative correlation with the sperm density and concentration (P=0.000). Conclusion: The levels of zinc, copper, iron and calcium in male seminal plasma have a close relationship with the sperm density and concentration, and sperm activity ability, which should be paid attention to in clinical practice.