To investigate the expression of microRNA-183 in ovarian cancer cells, and to study its effect on the proliferation and apoptosis of ovarian cancer cells.Methods: Fluorescence quantitative real-time PCR(RT-QPCR) was used to detect the expression level of Mir-183 gene in ovarian cancer and paracancer tissues.The proliferation of human ovarian cancer cells(SKOV3) and human ovarian cancer ovarian cancer cell(OVCAR3) lines introduced by Mir-183 simulant was determined by the thiazolam(MTT) assay.Cell apoptosis was detected by flow cytometry.Results: The mir-183 expression level(2.75±1.02) in ovarian cancer tissue was significant lower than that(5.43±1.27) in paracancer tissue(P<0.05).MTT and flow cytometry results showed that compared with that of cells without transfection, the proliferative activity of ovarian cancer cells after transfection had decreased significantly(P<0.05）, but the apoptosis rates of SKOV3 and OVCAR3(18.13±1.85% and 21.11±2.16%) had increased significantly(P<0.05).Conclusion: The expression level of microRNA-183 is closely related to the occurrence of ovarian cancer, and the high expression level of microRNA-183 can inhibit the proliferation of ovarian cancer cells and promote the apoptosis of ovarian cancer cells.

2021 Vol. 29　(11):　2261- [Abstract]（ 309 ） HTML (0 KB)  PDF  (0 KB)  ( 29 )

To explore the relationship between paternal smoking and spontaneous abortion of women with reproductive age in rural Yunnan. Method：195635 rural couples who had participated in the National Free Pre-pregnancy Checkups Project from 2015 to 2019 in Yunnan province were selected as study objects and were followed up till to their pregnancy outcomes. The relationship between paternal smoking and the spontaneous abortion of the women with reproductive age was analyzed. Result: Logistic regression analysis showed that paternal smoking increased the risk of spontaneous abortion of the women with reproductive age in rural Yunnan(OR=1.059, 95% CI: 1.011-1.108). The risk of spontaneous abortion of the women was consistent with the increasing trend of their husband's daily smoking(Z=435.408, P<0.001). The risk of spontaneous abortion of the women with their husband's daily smoking more than 20 cigarettes had increased significantly(OR=1.098, 95% CI: 1.025-1.176). Paternal quitting smoking during periceonceptional period was a protective factor of the spontaneous abortion(OR=0.862, 95% CI：0.801-0.928). Conclusion: Paternal smoking of the women increases their risk of spontaneous abortion in rural areas of Yunnan province, and the risk of spontaneous abortion increases with the increasing of paternal daily cigarette number. The risk of the spontaneous abortion significantly increases when the amount of smoking reached 20 cigarettes per day or more. Paternal quitting smoking during periceonceptional period is a protective factor of the spontaneous abortion of the women.

2021 Vol. 29　(11):　2270- [Abstract]（ 284 ） HTML (0 KB)  PDF  (0 KB)  ( 34 )

To study the development of reproductive health consulting service in primary maternal and child health care hospitals of Shanxi Province. Methods: From March to April in 2019, a questionnaire survey was conducted among 1989 staffs of maternal and child health care hospitals(MCH) or reproductive health consulting service clients in 117 counties（districts) maternal and child health care hospitals of Shanxi province. Results: 76.1% of the county（District) MCH hospitals had no special reproductive health consulting outpatient service. 59.8% of MCH hospitals had no formed a perfect management system. 64.1% of MCH hospitals had no any policy preference for the professional staffs that had passed the examination and obtained the professional qualification of reproductive health counselors. 59.3% of the staffs from MCH hospitals thought that the purpose of carrying out reproductive health consultation service was to prevent birth defects and improve the reproductive health literacy of the masses. More than 80% of the staffs thought that their hospitals focused on reproductive health counseling. Only 10.1% of the respondents believed that the hospital had got reproductive health counselor as the important content of professional title appointment. 60.2% of the clients were very satisfied with the reproductive health consultation services that provided by MCH hospitals. Conclusion: It is necessary to strengthen the service capacity of maternal and child health care institutions, to establish and improve the service system and working mechanism of reproductive health consulting service, to improve the guarantee and incentive mechanism of reproductive health consulting service personnel, and to actively explore the ways and means of reproductive health consulting service, so as to meet the people's reproductive health needs.

2021 Vol. 29　(11):　2278- [Abstract]（ 359 ） HTML (0 KB)  PDF  (0 KB)  ( 36 )

To explore glucocorticoid combined with norade for treating women with endometriosis（EMT) after laparoscopic surgery, and to study its influence on their estrogen level and latter pregnancy. Methods: 100 women with EMT were selected and divided into control group and observation group（50 cases in each group) according to the random number table method from May 2018 to may 2019. The women in the control group were treated with norade after laparoscopic surgery for 6 months, and the women in the observation group were treated with glucocorticoid combined with norade for 6 months. The clinical efficacy, estrogen level, pregnancy rate, and recurrence rate of EMT of the women were compared between the two groups. Results: The total clinical effective rate(96.0%) of the women in the observation group was significantly higher than that(80.0%) of the women in control group(P<0.05). After treatment, the estrogen level of the women in both groups had decreased significantly, and the decreasing degree of the women in the observation group was significantly more than that of the women in the control group(P<0.05). The pregnancy rate(74.0%) of the women within 1 year after operation in the observation group was significantly higher than that(52.0%) of the women in the control group, but the recurrence rate of EMT(2.0%) of the women within 1 year after operation in the observation group was significantly lower than that(10.0%) of the women in the control group(P<0.05). Conclusion: Glucocorticoid combined with norade for treating women with EMT after laparoscopic surgery can not only significantly improve the clinical efficacy and estrogen level, but also can increase the latter pregnancy rate and can reduce the recurrence rate of EMT.

2021 Vol. 29　(11):　2288- [Abstract]（ 304 ） HTML (0 KB)  PDF  (0 KB)  ( 34 )

To investigate the effect of laparoscopic myomectomy combined with the improved uterine artery occlusion for treating patients with uterine fibroids on their postoperative recovery, endocrine level, and prognosis. Methods: 86 patients with uterine fibroids were selected and were divided in study group and control group(between January 2018 and January 2021. 45 patients in the study group were treated by laparoscopic myomectomy combined with improved uterine artery occlusion, and 41 patients in the control group were treated by laparoscopic myomectomy. The postoperative recovery, the levels of follicle stimulating hormone（FSH), estradiol（E2), and luteinizing hormone（LH), and the endocrine recurrence and prognosis of the patients in the two groups were observed. Results: There was no significant difference in operative time of the patients between the two groups. The intraoperative blood loss, postoperative anal exhaust time,  and hospital stay time of the patients in the study group were significantly less than those of the patients in the control group(P<0.05). The changes of FSH, E2 and LH levels of the patients in the study group in 1 week after surgery were significantly less than those of the patients in the control group. The levels of FSH, E2, and LH of the patients in the study group in 3 weeks after surgery had no significant different from those before surgery, and the levels of FSH, E2, and LH levels of the patients in the control group in 6 weeks after surgery had no significant different from those before surgery(all P>0.05). The total incidence of complications(14.6%) and recurrence rate of uterine fibroids(4.9%) of the patients in the study group were significantly lower than those(35.6% and 20.0%) of the patients in the control group, and the rates of uterine morphology and menstruation returned to normal of the patients in the study group were significantly higher than those of the patients in the control group(all P<0.05). Conclusion: Laparoscopic myomectomy combined with the improved uterine artery occlusion for treating patients with uterine fibroids can effectively reduce their intraoperative blood loss, accelerate their postoperative recovery, reduce the influence on their sex hormone level and ovarian, decrease the risk of their postoperative complications and recurrence, and improve their prognosis.

2021 Vol. 29　(11):　2297- [Abstract]（ 295 ） HTML (0 KB)  PDF  (0 KB)  ( 34 )

2021 Vol. 29　(11):　2305- [Abstract]（ 301 ） HTML (0 KB)  PDF  (0 KB)  ( 32 )

To explore the clinical curative effect of ferrous eumanate and docusate sadium capsule combined with Jianpi shengxue granule for treating pregnant women with iron deficiency anemia（IDA). Methods: A total of 120 pregnant women with IDA were prospectively selected and randomly divided into three groups(50 cases in each group) by random number table. The women in group A were given polysaccharide-iron complex, the women in group B were given polysaccharide-iron complex and ferrous eumanate and docusate sadium capsule, and the women in group C were given polysaccharide-iron complex, ferrous eumanate and docusate sadium capsule, and Jianpi shengxue granule. The women in the three groups were treated for 4 weeks. The clinical efficacy of the women was compared among the three groups. Results: The total effective rate of the women in group B(88.0%) or group C(96.0%) was significantly higher than that(82.0%) of the women in group A(P<0.05). After treatment, there were no significant differences in the values of red blood cell, hemoglobin, and mean corpuscular volume, and the levels of serum iron, serum ferritin, and transferrin saturability of the women between group B and group C(P>0.05), but which of the women between group B and group C were all significantly higher than those of the women in group A(P<0.05). The incidence of constipation of the women in group C was 6%, which was significantly lower than that(24%) of the women in group A or that(26%) of the women in group B(P<0.05). There was no significant difference in perinatal outcomes of the women among the three groups(P>0.05). Conclusion: The anemia situation of the pregnant with IDA can be improved by routine iron supplementation treatment, and Jianpi shengxue granules used for the treatment additionally can further improve the therapeutic effect of anemia with good safety. The constipation status of the pregnant women can be improved by sodium ferrous fumarate capsule.

2021 Vol. 29　(11):　2312- [Abstract]（ 296 ） HTML (0 KB)  PDF  (0 KB)  ( 34 )

To explore the influence of post-abortion care services based on quality control circles on the knowledge of reproductive health, self-perceived burden, and contraceptive methods of women with high-risk abortion. Methods: 252 women with high-risk abortion were prospectively analyzed between March 2019 and March 2020. These women were divided into two groups according to the stratified block random method. 41 women in the control group were given routine service intervention, and 43 women in the observation group were given post-abortion care services in the quality control circle. The degree of reproductive health knowledge and choice of contraceptive methods of the women after the intervention, and self-perceived burden scale(SPBS) scores, and self-evaluation of depression scale(SDS) score, self-rating anxiety scale（SAS) score, and psychological resilience scale score in 1 and 2 months after intervention of the women were compared between the two groups. After the women were followed up for 6 months, the rates of repetitive abortion, the high-efficiency contraception used correctly, and the persistence contraception used in per sex life of the women were analyzed. Results: The SPBS score, SDS score, and SAS score of the women in the observation group in 1 and 2 months after intervention were significantly lower than those of the women in the control group, and the rate of non-contraceptive used(0%) of the women in the observation group was significantly lower than that(14.6%) of the women in the control group(P<0.05). The excellent rates of the optimism dimensionality, the strength dimensionality, the tenacity dimensionality, the score by mental resilience scale, and the degree of reproductive health knowledge of the women in the observation group were significantly higher than those of the women in the control group(P<0.05). During the 6 months of followed up, the rate of high effective contraception used correctly and contraception used in every sex life of the women in the observation group were significantly higher than those of the women in the control group, and the rate of repeated abortion of the women in the observation group was significantly lower(P<0.05). Conclusion: Providing post-abortion care services based on quality control circles for women with high-risk abortions can improve their self-perceived burden and increase the use of high efficient contraception.

2021 Vol. 29　(11):　2321- [Abstract]（ 246 ） HTML (0 KB)  PDF  (0 KB)  ( 31 )

To explore the clinical analgesic effect of ultrasound-guided transversus abdominisplane(TAP) block combined with diclofenac sodium suppository during laparoscopic hysterectomy, and to analyze its influence on cognitive level, and plasma interleukin-6(IL-6), C-reactive protein(CRP), cortisol(Cor) levels of patients. Methods: 100 patients who had undergone laparoscopic hysterectomy were selected as the research objects from October 2018 to October 2020. These patients were divided in observation group and control group(50 cases in each group) by the odd and even number labeling method. The patients in the control group had received ultrasound-guided TAP block anesthesia, and the patients in the observation group had received ultrasound-guided TAP block combined with diclofenac sodium suppository. The surgical indicators of the patients in the two groups were recorded. The differences of postoperative pain, cognitive function, and plasma IL-6, CRP, and Cor levels of the patients in the two groups were analyzed. Results: The dosage of postoperative fentanyl used, the spontaneous breathing recovery time, the wake-up time, and the time to get out of bed of the patients in the observation group were significantly less than those of the patients in the control group(P<0.05). In 48 hours after operation, the VAS scores of the patients in the two groups all had showed a trend of decreasing-increasing-decreasing, but the pain degree of the patients in the observation group was significantly lower than that of the patients in the control group(P<0.05). Compared with those before anesthesia, the levels of Tau protein, Tau/Aβ-42 value, and the IL-6, CRP, Cor levels of the patients in the two groups at 48 hours after operation had increased significantly, but the Aβ-42 level of the patients in the two groups had decreased significantly. At 48h after operation, the Tau protein level, Tau/Aβ-42 value, and the levels of IL-6, CRP, and Cor of the patients in the observation group were significantly lower than those of the patients in the control group(P<0.05). Conclusion: Ultrasound-guided TAP block combined with diclofenac sodium suppository for anesthesia during operation can improve intraoperative and postoperative anesthetic effect, decrease pain degree of the patients, reduce the negative impact of anesthesia on nerve function of the patients, and promote postoperative recovery of the patient.

2021 Vol. 29　(11):　2332- [Abstract]（ 263 ） HTML (0 KB)  PDF  (0 KB)  ( 33 )

To analyze the influence of newborns immediate skin contact with their mother after birth on the neonatal body temperature and breastfeeding, and the lactation yield and breast complication of puerpera. Methods: 236 full-term neonates were enrolled as the research objects from November 2018 to November 2020. According to random number table method, these neonates were divided into control group(n=118) and observation group(n=118). The neonates in the control group were given routine obstetric nursing measures, while the neonates in the observation group were given immediate skin contact with their mother after birth except to the routine obstetric nursing measures. The neonatal body temperature, the neonatal crying situation, the breastfeeding rate within 4d after born, the lactation yield and incidence of breast complications of the puerpera were compared between the two groups. Results: The neonatal body temperatures at 1h(36.5±2.7℃), 2h(36.9±2.8℃), and 3h(37.1±2.9℃) after birth in the observation group were significant higher than those(35.8±2.2℃, 36.1±2.4℃, and 36.4±2.7℃) in the control group. The number, frequency, and duration of neonatal crying in the observation group in 1h and 6h after birth were significant less than those in the control group. The rate of complete breastfeeding(71.4%) in the observation group was significant higher than that(50.9%) in the control group(all P<0.05), but there was no significant difference in mixed feeding rate between the two groups(P>0.05). The lactation yield of the puerpera in the observation group in 12h and 24h after delivery were significant higher than those of the puerpera in the control group. The incidence of mastitis(8.5%) and milk swelling(12.7%) of the puerpera in the observation group were significant lower than those(32.2% and 39.0%) of the puerpera in the control group(P<0.05). Conclusion: Newborns immediate skin contact with their mother after birth can increase neonatal temperature and improve breastfeeding can reduce times and duration of crying, can improve lactation yield of the puerpera, and can decrease the breast complication of the puerpera.

2021 Vol. 29　(11):　2341- [Abstract]（ 298 ） HTML (0 KB)  PDF  (0 KB)  ( 35 )

To analyze the serological screening results of toxoplasma gondii(TOX), rubella virus(RV), cytomegalovirus(CMV), and herpes simplex virus(HSV) of the pregnant women, and to study their relevant to their adverse pregnancy outcomes. Methods: 3065 pregnant women who underwent prenatal examination and TORCH serological detection from January 2019 to January 2020 were selected as the research objects, among them, there were 1725 women <35 years old and 1340 women ≥35 years old. These women were followed up, and 229 pregnant women were lost to follow-up. 2836 pregnant women who had completed the followed up were divided into group A(178 women with adverse pregnancy outcomes) and group B(2658 women with normal pregnancy outcomes). The serum IgM antibody of TOX, RV, CMV, and HSV -Ⅰ and HSV -Ⅱ were detected by colloidal gold method. Results: Among the 3065 pregnant women, 98 cases were positive for TORCH IgM, with the infection rate of 3.2%. The highest positive rate was CMV IgM(2.0%), followed by HSV-ⅠIgM(0.5%), HSV-Ⅱ IgM(0.4%), TOX IgM(0.2%), and RV IgM(0.2%). The positive rate of CMV IgM of the pregnant women in spring and autumn was significantly higher than that of the pregnant women in summer and winter. The positive rates of CMV IgM, HSV-ⅠIgM, and HSV-Ⅱ IgM of the pregnant women <35 years old were significantly higher than those of the pregnant women≥35 years old(P<0.05). The positive rates of TOX IgM, RV IgM, CMV IgM, HSV-Ⅰ IgM, and HSV-ⅡIgM of the women in group A were significantly higher than those of the women in group B(P<0.05). Logistic multifactor regression analysis showed that <35 years old, and the positive rates of TOX IgM, RV IgM, CMV IgM, HSV-Ⅰ IgM, and HSV-Ⅱ IgM were the independent risk factors of adverse pregnancy outcomes of the pregnant women(P<0.05). Conclusion: CMV infection of the pregnant women among TORCH infection is higher. TORCH infection is closely related to the season, age of the women, and the pregnancy outcomes. Therefore, screening TORCH infection of the pregnant women before pregnancy and during the first trimester of pregnancy is of great significance.

2021 Vol. 29　(11):　2350- [Abstract]（ 290 ） HTML (0 KB)  PDF  (0 KB)  ( 38 )

To explore the thyroid function and fetal free DNA level in peripheral blood of pregnant women with twin pregnancy. Methods: The pregnant women with twin pregnancy were selected and were divided 52 women with thyroid dysfunction in observation group and 52 women with normal thyroid function in control group from February 2019 to February 2020. The plasma genomic DNA magnetic bead method was used to extract and detect the fetal free DNA level in the peripheral blood of these women in the two groups. The blood glucose and blood lipid levels of the women in the two groups were detected by enzymic method. The incidences of gestational hypertension, anemia, and diabete of the women during pregnancy, and the fetal dysplasia and premature delivery were compared between the two groups. Results: The levels of fetal free DNA, total cholesterol, triglyceride, low density lipoprotein, cholesterol, and blood glucose of the women in the observation group were significantly higher than those of the women in the control group (P<0.05). There was no significant difference in the low-density lipoprotein level of the women between the two groups (P>0.05). The incidence (26.9%) of total complications, such as hypertension, anemia, diabetes, and fetal dysplasia of the women and the preterm birth rate (19.2%) in the observation group were significantly higher than those (11.5% and 1.9%) in the control group (P<0.05). Conclusion: The level of fetal free DNA in the peripheral blood of the women with twin pregnancy can reflect their thyroid function, blood glucose, and blood lipid levels. The women with twin pregnancy and dysfunction of thyroid function have more complications, and their pregnancy outcomes are not ideal.

2021 Vol. 29　(11):　2359- [Abstract]（ 341 ） HTML (0 KB)  PDF  (0 KB)  ( 35 )

To investigate the uterine ultrasound manifestations of women during different periods after cesarean section, and to analyze the influencing factors of their uterine scar diverticulum formation. Methods: 78 women after cesarean section were selected as the research objects, and they were all given vaginal ultrasound examination in 1 month, 3 months and 6 months after cesarean section. These women were divided into group A (58 women with good heal of uterine scar) and group B（20 women with uterine diverticulum). The changes of uterine ultrasound images of the women during different period after cesarean section were compared between the two groups. And combined with clinical data of the women with uterine scar diverticulum, the related influencing factors of uterine scar diverticulum formation were analyzed. Results: There were no significant different in the changes of the uterine length, width, and thickness of the women in 1, 3, and 6 months after cesarean section between the two groups (P>0.05). The ultrasonic characteristics of uterine scar of the women in group A mainly included the scar echo uniformly, the continuous and complete of uterine muscle layer incision without obvious defect, and the strong echo of uterine incision suture in some women. The ultrasonic manifestations of uterine scar of the women in group B were mainly the diverticulum with low echo or no echo, the thin and broken muscle layer at the incision, and the continuous and complete of the serosal layer without peripheral blood flow signal. The percentage of scar diverticulum of triangular was mainly 65.0%, and followed by that (20.0%) of the scar diverticulum of wedgeshaped. There were significant differences in the length, width, and depth of scar diverticulum of the women during different periods after cesarean section (P<0.05), but there were no significant differences in the residual muscle thickness and the distance between diverticulum and cervical endostoma (P>0.05). Multivariate analysis showed that the times of cesarean sections, the distance between incision diverticulum and cervical endostoma, the experience of the operator, the puerperal infection, the uterine position of the women were the influential factors of the scar diverticulum formation (P<0.05). Conclusion: The main ultrasonographic manifestations of uterine scar diverticulum after cesarean section of the women are the thinning of myometrium, the complete serosa layer, the hypoechoic or anechoic myometrial defect without obvious blood flow signal around. The size of scar diverticulum of the women decreases with the prolongation of postoperative time gradually. The scar diverticulum formation is mainly affected by the times of cesarean section, the experiences of the operator, the puerperal infection, the uterine posterior position, and other factors.

2021 Vol. 29　(11):　2367- [Abstract]（ 318 ） HTML (0 KB)  PDF  (0 KB)  ( 35 )

To investigate the changes of coagulation function and sex hormone level of patients with ectopic pregnancy. Methods: 85 patients with ectopic pregnancy were randomly selected in study group and 85 cases with normal intrauterine pregnancy were selected in control group from January to July 2019. The values of the activated partial thromboplastin time(APTT), prothrombin time(PT), thrombin time(TT), fibrinogen(FIB), and the levels ofβ-chorionic gonadotropin(β-hCG) and progesterone(P) of the patients were compared between the two groups. Results: The values of APTT, PT, and TT of the patients in the study group were 31.43s, 13.95s, and 22.86s, respectively, which were significant higher than those(29.17s, 11.75s, and 20.55s, respectively) of the women in the control group(P<0.05). The FIB value of the patients in the study group was 2.62±0.41 g/L, which was significant lower than that(3.41±0.40g/L) of the women in the control group(P<0.05). The levels of β-hCG and P of the patients in the study group were 2747.24U/L and 32.51 mol/L, which were significant lower than those(12639.71U/L and 78.64 mol/L) of the women in the control group(P<0.05). Conclusion: Compared with those of the women with normal intrauterine pregnancy, the coagulation function and hormone level of the patients with ectopic pregnancy during the first trimester of pregnancy has decrease, which should be paid more attention to during antenatal examination.

2021 Vol. 29　(11):　2376- [Abstract]（ 275 ） HTML (0 KB)  PDF  (0 KB)  ( 40 )

To explore the risk factors of the residual or positive lesions in resection margin after cervical cold knife conization (CKC) for treating the high-grade squamous intraepithelial lesion (HSIL) of cervix. Methods: The clinical data of 110 patients who underwent CKC for treating their HSIL of cervix from January 2017 to January 2019 were analyzed retrospectively. These patients were divided into group A (29 patients with supplementary surgery) and group B (81 patients without supplementary surgery) according to whether they were given supplementary surgery caused by residual or positive lesions in resection margin. The related factors of supplementary surgery after CKC were explored. Results: There were significant different in the proportions of parity >2 times (58.7% vs. 65.5%) and irregular menstruation (35.8% vs.43.2%) of the patients between the two groups. The proportions of the high risk HPV infection >2 types, the lesions of pathological classification with CIN III, the lesions of cervical transformation zone with CIN III, the lesions involved in glands, the lesions involved over 2 quadrants of cervix, and the cervical contact bleeding of the patients in group A were 55.2%, 69.0%, 62.1%, 58.6%, 51.7%, and 62.1%, respectively, which were significantly different from those (29.6%, 45.7%, 32.1%, 37.0%, 29.6%, 40.7%, respectively) of the patients in group B (P<0.05). There were no significant differences in the proportions of HPV infection and the ectopic degree of cervical cylindrical epithelium of the patients between the two groups (P>0.05). The proportions of the primary cervical conical incision width ≤15mm and primary conical incision volume by CKC ≤2cm3 of the patients in group A were significantly higher than those of the patients in group B, but the primary cervical resection depth of the patients in group A was significantly less than that of the patients in group B (P<0.05). Logistic multifactor regression analysis showed that high-risk HPV infection >2 types, the lesions of cervical transformation zone with CIN III, the lesions involved in glands, the lesions involved more than two quadrants of cervix, the cervical contact bleeding, the primary cervical conical incision width ≤15mm, primary conical incision volume by CKC ≤2cm3, and the cervical resection depth of the patients were the independent risk factors of supplementary surgery caused by residual or positive lesions in resection margin after CKC (P<0.05). Conclusion: The risk factors of supplementary surgery caused by residual or positive lesions in cervical resection margin after CKC should be paid enough attention to in clinic.

2021 Vol. 29　(11):　2384- [Abstract]（ 307 ） HTML (0 KB)  PDF  (0 KB)  ( 34 )

To investigate the relationship between cytomegalovirus (CMV) infection of women with spontaneous abortion and their levels of serum interferon (IFN), tumor necrosis factor-α(TNF-α), and interleukin-6 (IL-6). Methods: 167 women with spontaneous abortion from January 2018 to January 2020 were selected in study group retrospectively, and 80 normal pregnant women with unplanned pregnancy and no history of spontaneous abortion were selected in control group during the same period. Clinical data of the women in the two groups were collected and analyzed. Results: The positive rate of CMV-DNA (25.8%) of the women in the study group was significantly higher than that (12.5%) of the women in the control group, and the levels of serum IFN, TNF-α, and IL-6 of the women in the study group were significantly higher than those of the women in the control group (all P<0.05). The levels of serum IFN, TNF-α, and IL-6 of the women with once, 2 times, and ≥3 times of abortion in the study group were increased gradually (P<0.05). Spearman analysis showed that the levels of serun IFN, TNF-α, and IL-6 of the women were positive correlation with the times of abortions and the positive CMVV-DNA (P<0.05). The levels of IFN, TNF-α, and IL-6 of the women with positive CMV-IgM were significantly higher than those of the women with negative CMV-IgM (P<0.05). Binary logistic regression analysis showed that the levels of IFN, TNF-α and IL-6 were the influence factors of positive CMV-IgM of the women with spontaneous abortion (P<0.05). Conclusion: The levels of serum INF, TNF-α, and IL-6 of the women with spontaneous abortion elevate abnormally, which increase with the increasing of abortion times. The levels of serum IFN, TNF-α, and IL-6 of the women with positive CMV-IgM are higher than those of the women with negative CMV-IgM, and the levels of serum INF, TNF-α, and IL6 are the influence factors of positive CMV-IgM.

2021 Vol. 29　(11):　2393- [Abstract]（ 251 ） HTML (0 KB)  PDF  (0 KB)  ( 33 )

2021 Vol. 29　(11):　2403- [Abstract]（ 262 ） HTML (0 KB)  PDF  (0 KB)  ( 31 )

To analyze the changes of oxidative stress indexes of pregnant women with gestational diabetes mellitus (GDM) combined with hypertensive disorders of pregnancy (HDP), and to study their relationship with the insulin resistance of the women. Methods: From January 2016 to September 2019, 53 pregnant women with GDM and HDP were selected in group A, 106 pregnant women with GDM alone were selected in group B, and 106 pregnant women with normal blood pressure value and blood glucose level were selected in group C. The differences of oxidative stress indexes, such as MDA, AOPPs, LHP, T AOC of the women were compared among the three groups, and which’s correlation with the insulin resistance of the women was analyzed. Results: The levels of serum MDA, AOPPs, and LHP of the women in group C were the lowest, then followed by those of the women in group B, and which of the women in group A were the highest. The T AOC level of the women in group C were the highest, then followed by those of the women in group B, and which of the women in group A were the lowest (P＜0.05). The levels of serum MDA, AOPPs, and T AOC had predictive effects for GDM and HDP occurrences of the women (P＜0.05). The OGTT time, the area under of the blood glucose curve, the serum FPG and FINS levels, the HOMA-IR value of the women in group A and group B were significantly higher than those of the women in group C, but the HOMA-β value of the women in group A and group B was significantly lower. The OGTT time, the area under of the blood glucose curve, the serum FINS level, and the HOMA-IR value of the women in group A were significantly higher than those of the women in group B, and the HOMA-β value of the women in group A was significantly lower (all P＜0.05), but there was no significant difference in the FPG level of the women between group A and group B (P＞0.05). In group B, the serum MDA and AOPPs levels of the women were positively correlated with their HOMA-IR value (P＜0.05), the T-AOC level of the women was negatively correlated with their HOMA-IR value (P＜0.05), but the serum LHP level of the women was no correlated with their HOMA-IR value (P＞0.05). In group A, the serum MDA, AOPPs, and LHP levels of the women were positively correlated with their HOMA-IR value (P＜0.05), while the T AOC level of the women was negatively correlated with their HOMA-IR value (P＜0.05). Conclusion: The oxidative stress injury of the pregnant women with GDM and HDP is more serious, the pathophysiological states of GDM and HDP of the women are promoting each other.

2021 Vol. 29　(11):　2412- [Abstract]（ 278 ） HTML (0 KB)  PDF  (0 KB)  ( 37 )

To investigate the effects of the brain natriuretic peptide (BNP) and the fetal fibronectin (FFN) levels of the pregnant women with hypertensive disorder complicating pregnancy on their microvascular injury and cardiac function. Methods: The healthy pregnant women (in control group) and the pregnant women with hypertensive disorder complicating pregnancy (in study group) from 2016 to 2018 were collected retrospectively. The women in the study group were divided into group A1 (women with gestational hypertension), group A2 (women with mild preeclampsia), and group A3 (women with severe preeclampsia) according to different severity of hypertensive disorder complicating pregnancy. The BNP and FFN levels, the indexes of microvascular injury and cardiac function indexes of the women were compared among these groups. The correlation between the BNP and FFN levels of the women and their indexes of microvascular injury and cardiac function was analyzed. Results: The BNP and FFN levels of the women in the study group were significant higher than those of the women in the control group, and the BNP and FFN levels of the women in group A3 were the highest, while those of the women in A1 group were the lowest (P＜0.01). The values of LVEF and E/A of the women in the control group, group A1, group A2, and group A3 had decreased in turn. The value of CO of the women in the control group, group A1, group A2, and group A3 had increased in turn. With the elevation of the blood pressure value of the women, their CO value increased, but their CI value decreased. The RFF value of the women in group A1, group A2, and group A3 were significant lower than that of the women in the control group, while the values of NO, vWF, and TM of the women were significant higher than those of the women in the control group (all P＜0.05). Conclusion: The levels of BNP and FFN of the pregnant women with hypertensive disorder complicating pregnancy are related to their microvascular damage and cardiac function. The higher of the levels of BNP and FFN, the more serious the microvascular injury and the worse the ventricular diastolic function.

2021 Vol. 29　(11):　2420- [Abstract]（ 302 ） HTML (0 KB)  PDF  (0 KB)  ( 35 )

To investigate the expression of serum heat shock protein 70（HSP70) and the adverse pregnancy outcomes of pregnant women with preeclampsia. Methods: A total of 60 pregnant women with preeclampsia were selected, and were divided into group A（35 women with preeclampsia) and group B（25 women with severe preeclampsia) from October 2017 to October 2019. Another 40 healthy pregnant women who underwent regular physical examinations were selected in group C during the same period. The serum HSP70 levels of the women were compared among the three groups. And the relationship between the serum HSP70 levels of the women and their adverse pregnancy outcomes was analyzed. Results: The serum HSP70 level of the women in group B (5.47±2.16 ng/ml) was the highest, then followed by that（2.78±0.94 ng/ml）of the women in group A, and that (0.94±0.43 ng/ml) of the women in group C was the lowest (P<0.05). The incidence of the rate of fetal growth restriction, preterm delivery, postpartum hemorrhage, neonatal asphyxia, or fetal distress of the women in group B, in group A, and group C had decrease in turn significantly (all P<0.05). The incidence of the rate of fetal growth restriction, preterm delivery, postpartum hemorrhage, neonatal asphyxia, or fetal distress of the women with high level of serum HSP70 was significantly than that of the women with low level of serum HSP70 (P<0.05). Conclusion: The serum HSP70 level of the pregnant women with preeclampsia increases significantly, which increase as the disease worsen. The occurrences of adverse pregnancy outcomes of the women with high level of serum HSP70 also increase.

2021 Vol. 29　(11):　2428- [Abstract]（ 258 ） HTML (0 KB)  PDF  (0 KB)  ( 34 )

To explore the effects of hypothyroidism of the women during the third trimester of pregnancy on their glucose and lipid metabolism, heart function, and pregnancy outcomes. Methods: 70 pregnant women with hypothyroidism during the third trimester of pregnancy were selected in observation group from January 2019 to January 2020, and 70 healthy pregnant women during the third trimester of pregnancy were selected in control group during the same period. The indicators of thyroid hormone biochemical, blood glucose and blood lipid metabolism, and echocardiogram of the women were compared between the two groups, and the pregnancy outcomes of the women in the two groups were analyzed. Results: The levels of serum thyroid hormone and the serum FT3 and FT4 levels of the women in the observation group had significant different from those of the women in the control group. The levels of glucose and lipid metabolism indexes of the women in the observation group were significantly higher than those of the women in the control group (P＜0.05). The values of echocardiography index of the women in the observation group had significant different from those of the women in the control group (P＜0.05). The incidences of placental abnormality (11.4%), fetal growth restriction (12.9%), preterm birth (21.4%), and neonatal asphyxia (15.7%) in the observation group were significantly higher than those (1.4%, 1.4%, 8.6%, and 4.3%) in the control group (P＜0.05), but there were no significant differences in the incidences of adverse pregnancy, such as polyhydramnios, premature rupture of membranes, fetal distress, low birth weight, and macrosomia between the two groups (P＞0.05). Conclusion: Hypothyroidism of the women during the third trimester of pregnancy can cause their glucose and lipid metabolism dysfunction and affect their heart function, which can increase the incidence of adverse pregnancy outcomes. Clinically, timely intervention measures should be taken for the pregnant women with hypothyroidism to reduce the adverse effects of the disease on pregnant women and their fetuses.

2021 Vol. 29　(11):　2436- [Abstract]（ 334 ） HTML (0 KB)  PDF  (0 KB)  ( 39 )

To analyze the value of ultrasound imaging indexes combined with the levels of serum alpha fetal protein (AFP) and creatine kinase (CK) for placenta previa complicated with placental implantation. Methods: Pregnant women diagnosed with placenta previa by obstetric ultrasound were selected as the research subjects from March 2018 to December 2020. These women were divided into group A (the women with placental implantation disease) and group B (the women without placental implantation disease) according to whether the occurrence of placental implantation. The women in group A were further divided into group A1 (the women with placental adhesion) and group A2 (the women with confirmed placental implantation) according to the depth of villus invasion of the women. The women in these groups were given ultrasound examination and the serum AFP and CK detections. The women in group A were scored by ultrasound. Results: The serum AFP and CK levels of the women in group A were significantly higher than those of the women in group B, and the serum AFP and CK levels and the ultrasonic score of the women in group A1 were significantly lower than those of the women in group A2 (all P＜0.05). The serum AFP and CK levels had predictive value for placental implantation, which optimal cut-off value were 233.74ng/ mL and 153.26U/ mL, respectively. The serum AFP and CK levels and the ultrasound score of the women had predictive value for placental implantation type, which best cutoff values were 233.74ng/ ml, 153.26U/ml, and 8.49 points, respectively. The consistency of the serum AFP level, the CK level, or ultrasound examination alone for diagnosing placental implantation was common, and the consistency of the combined diagnosis of the serum AFP level, the CK level, and ultrasound examination for diagnosing placental implantation was better. The consistency of the serum AFP level, the CK level, or ultrasound examination alone for diagnosing placental implantation type was better, and the consistency of the combined diagnosis of the serum AFP level, the CK level, and ultrasound examination for diagnosing placental implantation type was perfect. Conclusion: Ultrasonography examination combined with the serum AFP and CK levels has good diagnostic efficacy for the placenta previa and placental implantation disease, and the placental implantation type, which can provide reference for the prenatal diagnosis of the pregnant women with placenta previa complicated with placental implantation.

2021 Vol. 29　(11):　2445- [Abstract]（ 242 ） HTML (0 KB)  PDF  (0 KB)  ( 33 )

 To study the changes of body mass index (BMI), thyroid function, and glucose metabolism of pregnant women with thyroxinemia during the third trimester of pregnancy, and to study their relevant to pregnancy outcomes of the women. Methods: The clinical data of 112 pregnant women during the third trimester of pregnancy from August 2017 to August 2020 were collected retrospectively. Among them, there were 112 pregnant women with thyroxinemia in experimental group and 113 healthy pregnant women in control group. The value of BMI, thyroid function, glucose metabolism change, and pregnancy outcomes of the women, and the neonatal outcomes were compared between the two groups. Results: The BMI value, and the levels of free triiodothyronine (FT3) and free thyroxine (FT4), fasting blood glucose, 1 h and 2 h postprandial blood glucose of the women in the experimental group were significantly higher than those of the women in the control group (all P<0.05). The incidences of the adverse pregnancy outcomes (12.5%, 14 cases) and the adverse neonatal outcomes (14.3% 16 cases) of the women in the experimental group were significantly higher than those (4.4%, 5 cases and 4.4%, 5 cases) in the control group (all P<0.05). Conclusion: BMI value of the women with thyroxinemia during the third trimester of pregnancy is higher, their levels of FT3 and FT4, and the glucose metabolism decrease, and their adverse neonatal outcomes and pregnancy outcomes increase.

2021 Vol. 29　(11):　2453- [Abstract]（ 306 ） HTML (0 KB)  PDF  (0 KB)  ( 34 )

To investigate the influence of bilateral internal iliac artery catheterization combined with cesarean section for treating patients with dangerous placenta previa and placenta implantation on their stress status and hysterectomy rate, and Apgar score of neonates.Methods: The clinical data of 103 pregnant women with dangerous placenta previa and placenta implantation from June 2018 to December 2019 were collected retrospectively.These women were divided into observation group(57 Cases) and the control group(46 cases) according to different treatment methods.The women in the control group were given conventional hemostasis measures combined with cesarean section treatment, and the women in the observation group were given bilateral internal iliac artery catheterization combined with cesarean section treatment.The intraoperative stress response level, surgical status, and the complications and hysterectomy rates of the women, and Apgar scores of the newborns were compared between the two groups.Results: The operative time, intraoperative blood loss, intraoperative blood transfusion, 24h postoperative blood loss of the women in the control group were 158.75± 23.56min, 2870.24± 366.28ml, 14.03± 5.34U, and 1106.73±320.34ml, which were significant higher than those(130.65±17.53min, 1776.34±210.24ml, 6.33±2.78U, and 908.34±135.25ml) of the women in the observation group.The HR(106.34±12.45 times/min), MAP(93.45±7.89 mmHG), serum cortisol(Cor) level(88.43±7.19 pg/ml), adrenocorticotrophic hormone(ACTH) level(32.17±6.78 ng/L), epinephrine(E) level(195.38±20.46ng/ml) of the women in the control group were significant higher than those(84.56±5.43times/min, 79.28±6.23 mmHG, 36.75±5.22 pg/ml, 13.56±3.21 ng/L, and 67.81±10.22 ng/ml) of the women in the observation group.The hysterectomy rate(19.6%, 9 cases), and postoperative complication rate(32.6%, 15 cases) of the women in the control group were significant higher than those(5.3）%, 3 cases, and 10.5%, 6 cases) of the women in the observation group(P>0.05).There were no significant differences in Apgar score of newborns at 1min and 5min after born between the two groups(P>0.05).Conclusion: The treatment of bilateral internal iliac artery catheterization combined with cesarean section for treating patients with dangerous placenta previa combined with placenta implantation has less intraoperative stress response, and has better surgical effectiveness, which can reduce the hysterectomy rate and can improve maternal and infant outcomes.

2021 Vol. 29　(11):　2461- [Abstract]（ 307 ） HTML (0 KB)  PDF  (0 KB)  ( 37 )

 To explore the relationship between the value of quantitative indexes of ultrasound contrast of the patients with endometrial carcinoma and the expression levels of matrix metalloproteinase(MMP) 2, MMP9, and vascular endothelial growth factor(VEGF) of the patients. Methods: 60 patients with endometrial cancer diagnosed by pathology were selected in group A, and 60 patients with hysteromyoma undergoing surgical resection were selected in group B from April 2018 to June 2020. The expression levels of MMP2, MMP9, and VEGF in excision tissues were detected by enzyme-linked immunosorbent assay(ELISA). The quantitative indexes of ultrasound contrast were monitored by color Doppler echocardiography. Pearson method was used to analyze the relationship between the value of quantitative indexes of ultrasound contrast and the expression levels of MMP 2, MMP9, and VEGF of the patients. Logistic regression analysis was used to analyze the influencing factors of endometrial cancer. Results: The values of mean transit time(MTT), and peak intensity(PI) of the patients in group A were significantly higher than those of the patients in group B(P<0.05), but the time to peak(TTP) of the patients in group A was significantly lower(P<0.05). The expression levels of MMP2, MMP9, and VEGF in endometrial carcinoma tissues of the patients in group A were significantly higher than those of the patients in group B(all P<0.05). The expression levels of MMP2, MMP9, and VEGF of the patients with pathological stages Ⅲ-Ⅳ, with lymph node metastasis, or with myometrial invasion >1/2 were significantly higher than those of the patients with pathological stagesⅠ-Ⅱ, without lymph node metastasis, or with myometrial invasion ≤1/2(P<0.05). The expression levels of MMP2, MMP9, and VEGF of the patients with endometrial carcinoma were positively correlated with their MTT value(P<0.05), but which were negatively correlated with their TTP value. The expression levels of MMP9 and VEGF of the patients with endometrial carcinoma were positively correlated with their PI value(all P<0.05). The MMP2 level of the patients with endometrial carcinoma was not significantly correlated to the PI value(P>0.05). The high expression levels of MMP2, MMP9, and VEGF of the patients were the risk factor of endometrial carcinoma. Conclusion: The expression levels of MMP2, MMP9, and VEGF in endometrial carcinoma tissues of the patients increase, which are closely related to the values of quantitative indexes of ultrasound contrast. It is suggested that the values of quantitative indexes of ultrasound contrast can reflect the expression levels of MMP2, MMP9, and VEGF in endometrial carcinoma tissues of the patients, which can provide certain reference for evaluating the condition of patients with endometrial carcinoma.

2021 Vol. 29　(11):　2469- [Abstract]（ 260 ） HTML (0 KB)  PDF  (0 KB)  ( 31 )

To explore the clinical effect of the optimizing service process of poster abortion contraception (PAC). Methods: The situation of immediate implementation of longterm reversible contraception（LARC) after induced abortion with standardized PAC process was analyzed and compared between from February to April 2019 and from February to April 2020. Results: The proportions of the induced abortions in outpatient operations of cynaecological clinic from February to April 2019 and from February to April 2020 were 78.9%（709/899) and 75.5%（764/1012), respectively, which had no significant different (P>0.05). The proportions of high-risk induced abortions of the women from February to April 2019 and from February to April 2020 were 46.8%（332/709) and 40.1% (306/764), respectively, which had significant different (P<0.05). The immediate implementation rate of LARC of the women from February to April 2019 and from February to April 2020 were 12.7% (90/709) and 15.3 (117/764), respectively, which had no significant different (P>0.05). Conclusion: The optimizing service process of PAC can effectively ensure the immediate implementation rate of LARC.

2021 Vol. 29　(11):　2478- [Abstract]（ 614 ） HTML (0 KB)  PDF  (0 KB)  ( 34 )