To explore the association of MTHFR (C677T and A1298C) single nucleotide polymorphisms with the risk of female infertility. Methods: Databases including PubMed, Web of Science, CNKI, Wanfang, VIP, and CBM were searched, and the searched time limit was from database establishment up to July 2021. The case-control studies about the association of MTHFR (C677T and A1298C) polymorphisms with the risk of female infertility were collected. State software was used to evaluate the association of MTHFR polymorphisms of the women with their risk of infertility by 95% confidence interval, odds ratio, publication bias, and heterogeneity in five models. Results: A total of 12 case-control studies were included. Meta-analysis showed that MTHFR gene (C677T and A1298C) polymorphisms of the women was not associated with their risk of infertility. 5 kinds models of MTHFR C677T were T vs. C (OR=1.11, 95%CI 0.97-1.26), TT vs.CC (OR=1.27, 95%CI 0.98-1.64), CT vs. CC (OR=1.08, 95%CI 0.90-1.31), (TT+CT) vs. CC (OR=1.12, 95%CI 0.93-1.36), and TT vs. (CC+CT) (OR=1.04, 95%CI 0.97-1.10), respectively, which suggested that MTHFR C677T gene polymorphism was not associated with the occurrence of female infertility. 5 kinds models of MTHFR A1298C were C vs. A (OR=1.00, 95%CI 0.92-1.09), CC vs. AA (OR=0.99, 95%CI 0.60-1.62), AC vs. AA (OR=1.00, 95%CI 0.89-1.12), (CC+AC) vs. AA (OR=1.00, 95%CI 0.90-1.11), and CC vs. (AC+AA) (OR=0.95, 95%CI 0.57-1.57)), respectively, which also suggested that MTHFR A1298C gene polymorphism was not associated with the occurrence of female infertility. Conclusion: MTHFR (C677T and A1298C) gene polymorphisms of the women maybe no associated with their risk of infertility.

2021 Vol. 29　(12):　2492- [Abstract]（ 339 ） HTML (0 KB)  PDF  (0 KB)  ( 28 )

To explore the effect of PTCH1 gene on the proliferation and apoptosis of ovarian cancer cells through the Hedgehog signaling pathway. Methods: RT-qPCR technology was used to detect the expression of PTCH1 gene in ovarian cancer tissues of 80 patients with ovarian cancer and in normal ovarian tissues of 80 women, in human ovarian cancer cell lines SKOV3 and A2780, and in human normal ovarian epithelial cell line IOSE80. The stable growth SKOV3 ovarian cancer cells were selected and were randomly divide into pIRES2-Ptch1 group, pIRES2-NC group, and pIRES2-Control group. The overexpression plasmid vector pIRES2-Ptch and the nonsense sequence pIRES2-NC were transfected into SKOV3 ovarian cancer cells, respectively. The cells in the control group were given nothing. RT-qPCR technology was used to detect the expression of PTCH1 gene in cells, and MTT test and flow cytometry were used to detect the proliferation and apoptosis of SKOV3 ovarian cancer cells. Results: The expression level of PTCH1（0.332±0.041）, and the positive rate of PTCH1 protein （23.8%） in ovarian cancer tissues were significantly lower than those (0.701±0.052 and 52.5%) in normal ovarian tissues (P<0.05). The expression levels of PTCH1 mRNA and PTCH1 protein in SKOV3 and A2780 ovarian cancer cells were significantly lower than those in IOSE80 normal ovarian epithelial cells (P<0.05). The expression level of PTCH1 in SKOV3 ovarian cancer cells in the pIRES2-Ptch1 group was significantly higher than that in the pIRES2-Scramble group and the control group (P<0.05). The proliferation activity of SKOV3 ovarian cancer cells transfected by PTCH1 gene overexpression had decreased in pIRES2-Ptch1 group, but which had increased in pires2-scramble group and in the blank control group (P<0.05). The apoptosis rate (16.22±1.08%) of the cells in piRES2-PTCH1 group was significantly higher than that (5.62±0.34%) of the cells in iRES2-scramble group and that (5.49±0.29%) of the cells in blank control group (P<0.05). Conclusion: PTCH1 gene can inhibit the expression of transcription factor Gli1 and the proliferation of ovarian cancer cells, and can induce the apoptosis of ovarian cancer cells by activating the Hedgehog signaling pathway. PTCH1 gene may be a potential diagnosis and treatment target of epithelial ovarian cancer.

2021 Vol. 29　(12):　2503- [Abstract]（ 457 ） HTML (0 KB)  PDF  (0 KB)  ( 30 )

To study the risk factors of premature ovarian failure (POF) of professional women. Methods: A case-control study was conducted on 276 professional women with POF in study group and 524 professional women in control group. Detailed medical histories of the women in the two groups were collected, and Eysenck Personality Questionnaire and Occupational stress inventory Revised Edition (OSI-R) were used in this survey. Multiple Logistic regression was used to analyze the main influencing factors of POF. Results: History of pelvic surgery (OR=2.117, 95%CI 1.304-3.435), history of mumps (OR=1.915, 95%CI 1.208-3.036), history of induced abortion (OR=1.128, 95%CI 1.107-1.150), psychoticism (OR=1.344, 95%CI 1.325-1.363), task ambiguity (OR=1.376, 95%CI 1.343-1.409)， and unclear boundaries of task (OR=1.263, 95%CI 1.229-1.299) of the women were the risk factors of their POF. Extraversion personality was the protective factor of their POF (OR=0.651, 95%CI 0.651-0.695). Conclusion: Pelvic surgery history, mumps history, abortion frequency, psychoticism, task ambiguity, and unclear boundaries of task of the women are the risk factors of their POF. Extraversion personality of the women is the protective factor of their POF.

2021 Vol. 29　(12):　2513- [Abstract]（ 287 ） HTML (0 KB)  PDF  (0 KB)  ( 33 )

To explore the therapeutic effect on menstrual recovery of short-acting oral contraceptives and sequential treatment of estrogen and progesterone for treating women after abortion, and to study its influence on endometrial receptivity of the women. Methods: A total of 120 women who underwent abortion were selected as the research subjects between April and December 2020. These women were divided into group A (40 women had treated by short-acting oral contraceptives), group B (40 women had treated by sequential treatment of estrogen and progesterone), and group C (40 women had no treated by any hormone drug) by random number table method. The vaginal bleeding time, the menstrual recovery time, the postoperative bleeding volume, the endometrial thickness, the menstrual condition (MDQ score), the levels of sex hormones ［estradiol (E2), luteinizing hormone (LH), follicle stimulating hormone (FSH)］, the complications and adverse reactions rates of the women were compared among the three groups. Results: The vaginal bleeding time, the menstrual recovery time, and the postoperative bleeding amount of the women in group A and group B were significantly lower than those of the women in group C. The endometrial thickness of the women in group B was significantly higher than that of the women in group A or group C. MDQ score of the women in group A and group B were significantly lower than that of the women in group C. The serum E2, LH, and FSH levels of the women in group A and group B were significantly higher than those of the women in group C. The total incidence of complications of the women in group A (5.0%) or group B (5.0%) was significantly lower than that (17.5%) of the women in group C (all P<0.05). There was no significant difference in the incidence of adverse reactions of the women among the three groups (P>0.05). Conclusion: Short-acting oral contraceptives and sequential treatment of estrogen and progesterone for treating the women after abortion both can significantly shorten their vaginal bleeding duration and can promote their menstrual recovery, and sequential treatment of estrogen and progesterone can improve the endometrial receptivity of the women.

2021 Vol. 29　(12):　2522- [Abstract]（ 322 ） HTML (0 KB)  PDF  (0 KB)  ( 31 )

To explore the efficacy of self-made Bushen huayu recipe combined with gestrinone for treating women with endometriosis (EMs) of renal deficiency and blood stasis type, and to study its mechanism of action. Methods: 88 women with EMs of renal deficiency and blood stasis type were selected as the observation objects, and were divided into two groups by convenience sampling method. 44 women in the control group were treated with gestrinone for 3 months, and 44 women in the observation group were treated with self-made Bushen huayu recipe combined with gestrinone for 3 months. The quantitative scores of TCM symptoms before and after treatment, such as abdominal pain during menstruation, lumbosacral pain, oligomenorrhea, dizziness, insomnia, and hypaphrodisia, the levels of the estradiol (E2), follicle stimulating hormone (FSH), and luteinizing hormone (LH), the levels of serum cancer antigen 125 (CA125), prostaglandin E2 (PGE2), prostaglandin F2α (PGF2α), vascular endothelial growth factor (VEGF), and matrix metalloproteinase-9 (MMP-9), the values of high and low shear viscosity of whole blood, plasma viscosity, and hematocrit, and the dysmenorrhea score of the women in the two groups were observed and analyzed. The clinical efficacy and safety of the treatment of the women in the two groups were evaluated. The recurrence rate was counted after 6 months of followed up. Results: After treatment, the scores of TCM symptoms of the women in both groups had decreased significantly, and which of the women in the observation group were significantly lower than those of the women in the control group. The levels of E2, LH, FSH, PGE2 and PGF2αof the women in both groups had decreased significantly after treatment, and which of the women in the observation group were significantly lower than those of the women in the control group. After treatment, the levels of VEFG and MMP-9 of the women in the observation group had no significantly change, but which of the women in the control group had decreased significantly. The values of high and low shear viscosity of whole blood, plasma viscosity, and hematocrit, and the visual analogue scale of pain of the women in both groups had decreased significantly after treatment, and which of the women in the observation group were significantly lower than those of the women in the control group (all P<0.05). The total effective rate (93.2%) of the women in the observation group was significantly higher than that (81.8%) of the women in the control group (P<0.05), and there was no significant difference in the adverse reaction rate (15.9% vs. 20.5%) of the women between the two groups (P>0.05). The recurrence rate (2.3%) of the women in the observation group was significantly lower than that (11.4%) of the women in the control group (P<0.05). Conclusion: Self-made self-made Bushen huayu recipe combined with gestrinone for treating women with endometriosis of renal deficiency and blood stasis type can alleviate the symptoms of EMs and has significant clinical efficacy, which mechanism may be related to the regulation of sex hormone levels, serum biochemical indicators, and hemorheology of these women.

2021 Vol. 29　(12):　2530- [Abstract]（ 307 ） HTML (0 KB)  PDF  (0 KB)  ( 30 )

To investigate the influence of preemptive analgesia by dexmedetomidine for treating women during laparoscopic hysterectomy on their postoperative pain and the expressions of their serum exosome long non-coding RNA colon cancer associated transcript 1 (lncRNA CCAT1). Methods: From June 2018 to March 2020, 80 women who wanted laparoscopic hysterectomy were selected as the research objects, and were randomly divided into two groups according to the random number table. 40 women in the control group were given conventional analgesia, while 40 women in the study group were given dexmedetomidine for preemptive analgesia. The visual analogue scale (VAS) score, Ramsay score, the serum lncRNA CCAT1 expression, and the adverse reactions of the women in the two groups were observed. Results: VAS score and Ramsay score of the women in the two groups had no significant different among the different postoperative time points (P>0.05), but VAS score of the women in the study group at 4h, 8h, 12h, or 24h after surgery was significantly lower than that of the women in the control group, and Ramsay score of the women in the study group at 4h, 8h, 12h, or 24h after surgery was significantly higher than that of the women in the control group (P<0.05). The expression level of the serum exosomes lncRNA CCAT1 (0.54±0.22) of the women in the study group after operation was significantly lower than that (0.96±0.31) of the women in the control group (P<0.05). There was no significant difference in the incidence of postoperative bradycardia (0 vs.5.0%) of the women between the two groups, and the incidences of nausea (7.5%) and vomiting (5.0%) of the women in the study group were significantly lower than those (27.5% and 20.0%) of the women in the control group (P<0.05). Conclusion: Preemptive analgesia by dexmedetomidine for treating women during laparoscopic hysterectomy can effectively relieve the pain, enhance the analgesic effect, reduce the expression level of serum lncRNA CCAT1 after operation, and reduce the occurrence of adverse reactions.

2021 Vol. 29　(12):　2542- [Abstract]（ 325 ） HTML (0 KB)  PDF  (0 KB)  ( 29 )

To investigate the relationship between the levels of serum fibrin gel protein-3 (ficolin-3), adiponectin (ADP), and pregnancy associated protein-A (PAPP-A) and glycolipid metabolism of pregnant women with gestational diabetes mellitus (GDM) and their glucose and lipid metabolism, and to study their values for predicting the maternal and infant outcomes. Methods: 112 pregnant women with GDM were prospectively included in study group and 103 pregnant women with healthy prenatal examination were selected in control group from May 2019 to May 2021. The correlations between the levels of serum ficolin-3, ADP, PAPP-A of the women and the levels of their fasting blood glucose (FBG), fasting insulin (FINS), triglyceride (TG), total cholesterol (TC), high density lipoprotein cholesterol (HDL-C), and low density lipoprotein cholesterol (LDL-C), and the value of homeostasis model assessment insulin resistance index (HOMA-IR) were analyzed. Logstic regression analysis was used to analyze the influence of the levels of ficolin-3, ADP, and PAPP-A of the women on the maternal and infant outcomes. The area under receiver operating curve (ROC) was used to analyze the value of the levels of ficolin-3, ADP, and PAPP-A for predicting the maternal and infant outcomes. Results: The levels of ficolin-3 and PAPP-A, and the levels of FBG, FINS, HOMA-IR, TG, and LDL-C higher of the women in the study group than those of the women in the control group, the levels of ADP and HDL-C of the women in the study group were significantly lower (all P<0.05). The ficolin-3 level of the women with GDM was negatively correlated with their HDL-C level. The ADP level of the women with GDM was negatively correlated with their FBG and FINS levels, and their HOMA-IR value. The PAPP-A level of the women with GDM was positively correlated with their FINS level, but was negatively correlated with their HDL-C level (all P<0.05). In the study group, the ficolin-3 and PAPP-A levels of the women with placenta previa, placental abruption, cesarean section, or premature delivery were significantly higher than those of the women with normal pregnancy outcomes, and the ADP level of those women was significantly lower than those of the women with normal pregnancy outcomes. In the study group, the ficolin-3 and PAPP-A levels of the women with fetal distress, macrosomia, premature rupture of membranes, low birth weight, neonatal hypoglycemia, or neonatal asphyxia were significantly higher than those of the women with normal pregnancy outcomes, and the ADP level of those women was significantly lower than those of the women with normal pregnancy outcomes (P<0.05). The levels of FBG, FINS, FIColin-3, PAPP-A, and ADP were the independent risk factors of the adverse outcomes of the women with GDM. ROC analysis showed that the AUC of PAPP-A, Ficolin-3, and ADP for predicting GDM were 0.85, 0.790, and 0.701, respectively. Conclusion: The abnormal levels of serum ficolin-3, ADP, and PAPP-A are related to the occurrence of GDM, and are also related to the indexes of glucose and lipid metabolism. The detections of the levels of serum ficolin-3, ADP, and PAPP-A during the first trimester of pregnancy can provide references for predicting the occurrence of GDM and the adverse pregnancy outcomes.

2021 Vol. 29　(12):　2551- [Abstract]（ 289 ） HTML (0 KB)  PDF  (0 KB)  ( 31 )

To investigate the relationship between the levels of serum copeptin and galectin-3 of the pregnant women with hypertensive disorder complicating pregnancy (HDCP) and their decline of the left ventricular function. Methods: 150 pregnant women with HDCP were selected in observation group and were divided in group A (89 cases with HDCP) and group B (61 cases with preeclampsia) from June 2020 to May 2021. 100 normal pregnant women were selected in control group during the same period. The levels of serum peptide and galectin-3 of the women in the three groups were measured, and the indexes of left ventricular function were measured by ultrasound and were calculated. The basic data, the levels of serum peptide and galectin-3, the left ventricular function index of the women were compared among the three groups. Pearson correlation analysis and Logistic regression analysis were also performed. Results: The values of the systolic and diastolic blood pressure of the women in group B were significantly higher of the women in group A and in the control group (P＜0.05). The expression levels of serum copeptin and galectin-3, B-type natriuretic peptide (BNP), and lactate dehydrogenase (LDH) of the women in group B were significantly higher than those of the women in group A and in the control group (P＜0.05). The values of maximum early diastolic flow rate/maximum late diastolic velocity (E/A), and the early diastolic rapid filling fraction (RFF) of the women in group B were significantly lower than those of the women in group A and in the control group (P＜0.05). With the aggravation of HDCP, the expression levels of serum copeptin galectin-3, BNP, LDH of the women in group B were significantly higher than those of the women in group A. The serum copeptin and galectin-3 levels of the women with HDCP were no correlated with their cardiac output (CO), left ventricular ejection fraction (LVEF), and cardiac index (CI) values (P＞0.05), but were negatively correlated with their E/A and RFF values (P＜0.05). Logistic multifactor regression analysis showed that the serum copeptin and galectin-3, BNP, and LDH levels, and the values of systolic and diastolic blood pressure of the pregnant women with HDCP were the independent risk factors of their left ventricular diastolic function injury, which OR (95% CI) were 1.375 (1.209-1.566), 1.311 (1.122-1.535), 1.063 (1.021-1.107), 1.074 (1.033-1.115), 1042 (1.020-1.064), and 1.052(1.018-1.086), respectively. Conclusion: The levels of serum copeptin and galectin-3 of the pregnant women with HDCP are related to the severity of HDCP, both of which are the risk factors of the decline of the left ventricular function of these women and may be involved in the injury of left ventricular function of these women.

2021 Vol. 29　(12):　2561- [Abstract]（ 291 ） HTML (0 KB)  PDF  (0 KB)  ( 29 )

To analyze the anesthesia effect of epidural anesthesia assisted by sevoflurane inhalation of pregnant women with gestational hypertension during cesarean section, and to study its influence on the maternal hemodynamics. Methods: A total of 90 pregnant women with gestational hypertension who underwent cesarean section were selected and randomly divided into two groups (45 cases in each group) by random number table from March 2018 to March 2021. The women in the control group were anesthetized with epidural nerve block, while the women in the experimental group were anesthetized with epidural nerve block assisted by sevoflurane inhalation. The anesthesia effect and maternal hemodynamic change of the women before anesthesia (T1), 10 min after anesthesia (T2), fetal delivery (T3), and at the end of surgery (T4) were analyzed and compared between the two groups. Results: There were no significant differences in the intraoperative blood loss, the duration of fetal delivery, the fetal Apgar score at 1 min and 5min after born, and the umbilical cord blood pH value of the women between the two groups (P>0.05). The proportion of class III, II, and I of anesthesia of the women in the experimental group were 44.4%, 35.6%, and 20.0%, respectively. The proportion of class III, II, and I of anesthesia of the women in the control group were 20.0%, 40.0%, and 40.0%, respectively. The anesthesia effect of the women in the experimental group was significantly better than that of the women in the control group (χ2=7.290, P<0.05). There were no significant differences in the values of MAP, HR, CO, CI, and other hemodynamic indexes of the women at T1 between the two groups (P>0.05). The values of MAP, HR, CO, and CI of the women in the experimental group at T2, T3 and T4 were significantly higher than those of the women in the control group, and the incidence of adverse reactions during and after anesthesia (11.1%) of the women in the experimental group was significantly lower than that (28.9%) of the women in the control group (P<0.05). Conclusion: Epidural anesthesia assisted by sevoflurane inhalation for cesarean section of pregnant women with gestational hypertension during cesarean section can work rapidly with less adverse reactions, which can effectively stabilize the maternal hemodynamics during operation.

2021 Vol. 29　(12):　2570- [Abstract]（ 305 ） HTML (0 KB)  PDF  (0 KB)  ( 30 )

2021 Vol. 29　(12):　2580- [Abstract]（ 341 ） HTML (0 KB)  PDF  (0 KB)  ( 33 )

2021 Vol. 29　(12):　2588- [Abstract]（ 325 ） HTML (0 KB)  PDF  (0 KB)  ( 32 )

To investigate the changes of serum fetal liver kinase（Flk)-1 and cyclooxygenase-2（COX-2) levels of women with endometriosis（EMs), and to study their correlation with the degree of pain and the EMs condition of the women. Methods: A total of 112 women with EMs were selected in study group between January 2019 to December 2020, and which were further divided into group A (23 women with painless), group B (26 women with mild pain), group C (45 women with moderate pain), and group D (18 women with severe pain) according to the degree of pelvic pain by Visual Analog Scale（VAS), and which were also further divided into group E (16 women with stage I of EMs ), group F (44 women with stage II of EMs), group G (29 women with stage III of EMs), and group H (23 women with stage IV of EMs) according to the EMs condition by the American Fertility Association's revised staging method (r-AFS). 70 healthy women of childbearing age were selected in control group. The serum levels of Flk-1 and COX-2 of the women in these group were detected, and their correlation with the degree of pain of the women were analyzed. Results: The levels of serum FLK-1 (26.60±7.44ng/ml) and COX-2 (3.16±0.53ng/ml) of the women in the study group were significantly higher than those (6.52±1.13ng/ml and 1.42±0.31ng/ml) of the women in the control group (P<0.05). The levels of Flk-1 and COX-2 of the women in the study group had increased with their increasing VAS score and R-AFS stage (P<0.05). Linear correlation analysis showed that serum FLK-1 level of the women was positively correlated with their serum COX-2 level, and the levels of Flk-1 and COX-2 of the women were positively correlated with their VAS score and R-AFS stage (R =0.781, 0.658, 0.792, and 0.708, P<0.05). Conclusion: The women with EMs have obvious high expressions of the serum Flk-1 and COX-2, which are closely related to their EMs stage and their degree of pain, which can provide reference for clinical evaluation and treatment of EMs.

2021 Vol. 29　(12):　2598- [Abstract]（ 369 ） HTML (0 KB)  PDF  (0 KB)  ( 33 )

2021 Vol. 29　(12):　2606- [Abstract]（ 347 ） HTML (0 KB)  PDF  (0 KB)  ( 35 )

To explore the levels and clinical significance of serum osteocalcin, β isomer of C-terminal telopeptide of type I collagen (β-CTX), calcium (Ca), phosphorus (P), and 25-hydroxyvitamin D (25(OH)D) of women with menopausal osteoporosis. Methods: A total of 257 women with menopausal osteoporosis or low bone mass confirmed by bone densitometer were enrolled between May and December 2020. According to different bone density, these women were divided into group A (68 cases with osteoporosis, T≤-2.5) and group B（124 cases with low bone mass, -2.5 <T<-1). 65 normal women (T≥-1) were selected in group C. The clinical data of the women in the three groups were collected. The levels of serum Ca, P, OC, β-CTX, and 25(OH)D of the women in the three groups were detected. The diagnostic value of all indexes for osteoporosis of women with menopausal osteoporosis was analyzed by receiver operating characteristic（ROC) curve. Results: There was no significant difference in the blood P level of the women among group A, group B, and group C (P>0.05). The serum Ca level of the women in group C, group B, and group A had decreased gradually (P<0.05). The OC and β-CTX levels of the women in group A were significantly higher than those of the women in group C, but the 25(OH)D level of the women in group A was significantly lower. The OC and β-CTX levels of the women in group B were significantly higher than those of the women in group C (all P<0.05). ROC curve analysis showed that the AUC of the level of serum Ca, OC, β-CTX, and 25(OH) D for diagnosing osteoporosis of menopausal women were 0.746, 0.774, 0.658, and 0.787, respectively, and the AUC of the combined of the levels of serum Ca, OC, β-CTX, and 25(OH) D for diagnosing osteoporosis of menopausal women was 0.902, which was the highest. Conclusion: The levels changes of serum Ca, OC, β-CTX, and 25(OH)D of women may be related to their occurrence of menopausal osteoporosis. The combined levels of serum Ca, OC, β-CTX, and 25(OH) D for diagnosing menopausal osteoporosis has high clinical value.

2021 Vol. 29　(12):　2616- [Abstract]（ 342 ） HTML (0 KB)  PDF  (0 KB)  ( 31 )

To explore the influence of different methods for treating infertile patients with hydrosalpinx on their ovarian reserve function and the pregnancy outcomes after IVF-ET. Methods: 171 infertility patients between March 2018 and May 2019 were analyzed retrospectively. These patients were divided into three groups according to the surgical methods. Among these patients, 58 cases in group A were treated by proximal tubal ligation combined with distal end tubal salpingostomy, 56 cases in group B were treated with salpingostomy, and 57 cases in group C were treated with salpingectomy. The ovarian reserve function, the ovarian volume, the total Gn dosage, the endometrial thickness, the time of Gn used, and the assisted pregnancy outcomes of the patients were compared among the three groups. The incidence of complications of the patients in the three groups during hospitalization was counted. Results: There were no significant differences in the levels of follicle stimulating hormone (FSH), estradiol (E2), and luteinizing hormone (LH), and the number of sinus follicles of the patients after treatment between group A and group B (P>0.05). In the 1st and 3rd menstrual cycle after treatment, the FSH and E2 levels of the patients in group A and group B were significantly lower than those of the patients in group C, but the LH level and the number of sinus follicles of the patients in group A and group B were significantly higher (all P<0.05). The ovarian volume (5.41±1.25 ml) of the patients in group A was significantly lower than that of the patients in group B or group C. The fertilization rate, the high-quality embryo rate, the clinical pregnancy rate, and the live birth rate of the patients were significantly higher than those of the patients in group B and group C. The complication rate (3.5%) of the patients in group A was significantly lower than that (14.0%) of the patients in group C (all P<0.05). Conclusion: Compared with that of the salpingectomy, the proximal tubal ligation combined with distal end tubal salpingostomy, and the salpingostomy for treating infertile patients with hydrosalpinx have less adverse influence on their ovarian reserve function. The proximal tubal ligation combined with distal end tubal salpingostomy also can increase the assisted pregnancy rate of the patients.

2021 Vol. 29　(12):　2623- [Abstract]（ 267 ） HTML (0 KB)  PDF  (0 KB)  ( 30 )

To analyze the value of color Doppler ultrasound and magnetic resonance imaging (MRI) for screening fetal congenital malformation of heart (CMH), and to explore the relationship between fetal CMH and their chromosomal abnormalities. Methods: 178 pregnant women with suspected fetal CMH by routine twodimensional ultrasound examination during prenatal examination were selected from January 2017 to January 2021. These women had accepted further color ultrasound examination, MRI examination, and the umbilical cord puncture for chromosome examination, respectively. Follow-up was conducted to compare the accuracy of color ultrasound and MRI examinations. The fetal karyotypes of chromosomal abnormalities were counted and the situation of chromosomal abnormalities of fetuses with CMH was analyzed. Results: 178 fetuses with suspected CMH were confirmed by delivery, autopsy, or operation. There were 145 (81.5%) fetuses diagnosed with CMH, 21 (11.8%) cases with diaphragmatic hernia, and 12 (6.7%) cases with pleural effusion. There was no significant difference in the diagnostic rate (79.2%, 141 cases vs. 80.3%, 143 cases) between the examinations of color ultrasonography and MRI (P=0.792). There were 41 (23.0%) cases with chromosomal abnormalities, which included 36 (20.2%) cases with chromosomal number abnormalities and 5 (2.8%) cases with chromosomal structure abnormalities. Among the 41 fetuses with chromosomal abnormalities, there were 29 cases with CMH, 12 cases with diaphragmatic hernia or pleural effusion. When chromosomal karyotype abnormality was included in the regression analysis equation of the influencing factors of fetal CMH, the chromosomal abnormality was an independent risk factor of fetal malformation (OR=2.286, P=0.048), and the fetal chromosomal abnormality was correlated with the fetal malformation (r=-0.151, P=0.044). Conclusion: Both color Doppler ultrasound and MRI have ideal screening efficacy for fetal CMH. The fetal CMH is related to their chromosomal abnormalities, and improving chromosomal karyotype screening efficiency can further improve the diagnosis rate of fetal CMH.

2021 Vol. 29　(12):　2633- [Abstract]（ 320 ） HTML (0 KB)  PDF  (0 KB)  ( 35 )

To explore the expression and clinical significance of glucose transporter 1（Glut-1) and sulfate transferase 1A3（SULT1A3) in uterine fibroids tissue. Methods: The uterine fibroids tissue specimens of 90 women after uterine myomectomy (in group A) and the myometrium tissue specimens of 78 women with uterine prolapse and without other uterine lesions after total hysterectomy (in group B) were collected between February 2019 and February 2021. Immunohistochemistry was used to detect the Glut-1 and SULT1A3 protein expressions in tissue samples. The relationship between the levels of Glut-1 protein and SULT1A3 protein of the women with uterine myomectomy and the clinicopathological characteristics of the women was analyzed. Kendall correlation analysis was used to analyze the correlation between the Glut-1 protein level and the SULT1A3 protein level. Results: The expression of Glut-1 protein in uterine fibroids tissues was significantly higher than that in normal myometrium tissues, but the expression of ULT1A3 protein in uterine fibroids tissues was significantly lower than that in normal myometrium tissues (all P<0.05). The Glut-1 and SULT1A3 proteins levels had showed no significant differences in the myometrium tissues among the women with different age, among the women with different body mass index, or among the women with different fibroid diameter (P>0.05). The expressions of Glut-1 protein and ULT1A3 protein in uterine fibroids tissues of the women with FIGO type 2 of uterine fibroids were higher (P<0.01). The expression of Glut-1 protein in uterine fibroids tissues of the women without menopause was high, and the expression of SULT1A3 protein in uterine fibroids tissues of the women with menopause was higher (P<0.05). Correlation analysis showed that the glut-1 protein level in uterine fibroids tissues was negatively correlated with the SULT1A3 protein level in uterine fibroids (P<0.01). Conclusion: The expression level of Glut-1 protein in uterine fibroids tissue is higher than that in normal myometrium tissues, while the expression level of SULT1A3 protein in uterine fibroids tissue is lower than that in normal myometrium tissues. Both of the expression levels of Glut-1 protein and SULT1A3 protein in uterine fibroids tissue of the women are related to their FIGO type of uterine fibroids and are related to their menopausal status, which are expected to become one of the new targets for treating uterine fibroids.

2021 Vol. 29　(12):　2641- [Abstract]（ 347 ） HTML (0 KB)  PDF  (0 KB)  ( 33 )

2021 Vol. 29　(12):　2650- [Abstract]（ 335 ） HTML (0 KB)  PDF  (0 KB)  ( 34 )

2021 Vol. 29　(12):　2659- [Abstract]（ 347 ） HTML (0 KB)  PDF  (0 KB)  ( 34 )

To compare the different effect of sufentanil or remifentanil combined with propofol during painless induced abortion. Methods: 126 women who underwent painless abortion were selected and were divided into group A (63 women with sufentanil combined with propofol) and group B (63 women with remifentanil combined with propofol) by random number table method from August 2017 to December 2018. The effect of anesthesia, the vital signs before and after anesthesia, and the rate of adverse reactions of the women were compared between the two groups. Results: There were no significant differences in the rate of qualified anesthetic effect (96.8%, 61 cases vs. 98.4%, 62 cases) of the women between the two groups (P＞0.05). After anesthesia, there were no significant differences in the values of the heart rate and the oxygen saturation of the women between the two groups, but the mean arterial pressure value of the women in group A was significantly higher than that of the women in group B (P＜0.05). In group A, there were 4 women with dizziness, 2 women with nausea and vomiting, and 2 women with bradycardia, and the total incidence of adverse events of the women was 12.7%. In group B, there were 3 women with dizziness, 5 women with nausea and vomiting and 1 case woman with bradycardia, and the total incidence of adverse events of the women was 14.3%. There was no significant difference in the total incidence of adverse events of the women between the two groups (P＜0.05). Conclusion: The anesthetic effect of sufentanil or remifentanil combined with propofol during painless induced abortion is satisfaction. The anesthetic effect and the total incidence of adverse events of the women with sufentanil combined with propofol for anesthesia has no significant different from those of remifentanil combined with propofol.

2021 Vol. 29　(12):　2668- [Abstract]（ 334 ） HTML (0 KB)  PDF  (0 KB)  ( 29 )

To investigate the relationship between the levels of serum interleukin-17 (IL-17), β-HCG, estradiol (E2) and progesterone (P) of the pregnant women during the first trimester of pregnancy and their pregnancy outcomes. Methods: The clinical data of 590 pregnant women during the first trimester of pregnancy from August 2019 to February 2021 were collected and were divided into group A (369 women with normal pregnancy outcomes) and group B (221 women with adverse pregnancy outcomes) according to their pregnancy outcomes. Chemiluminescence method was used to detect the levels of serum IL-17, E2, P and β-HCG of the women in the two groups. Results: There were 380 (66.4%) women with one or multiple of the levels of IL-17, E2, P, and βHCG, which included 13.2% women with abnormal IL-17 level only, 12.5% women with abnormal E2 level only, 13.4% women with abnormal P level only, and 12.2% women with abnormal β-HCG level only. There were 13.1% women with ≥2 abnormality of the levels of IL-17, E2, P, and β-HCG. The incidence of the adverse pregnancy outcomes of the pregnant women with the abnormal increase of the IL-17 and β-HCG levels, and the abnormal decrease of E2 and P levels had increased significantly (P＜0.05). Conclusion: The probability of adverse pregnancy outcomes of the pregnant women with the abnormal levels of serum IL-17, E2, P, and β-HCG during the first trimester of pregnancy increases, which should be paid attention in clinic.

2021 Vol. 29　(12):　2675- [Abstract]（ 323 ） HTML (0 KB)  PDF  (0 KB)  ( 33 )

To investigate the values of the serum high mobility group protein（HMGB1), soluble CD40L（sCD40L), placental growth factor（PIGF), and soluble vascular growth factor receptor-1（sFlt-1) levels of the pregnant women for predicting their preeclampsia (PE). Methods: 92 pregnant women with PE were selected in study group and were divided into group A (50 women with mild PE) and group B (42 women with severe PE) according to the severity of PE from January 2019 to December 2020. 50 normal pregnant women were selected in control group during the same period. The levels of the serum HMGB1, sCD40L, PIGF, and sFlt-1 of the women in the three groups were measured by enzyme-linked immunoassay（ELISA). Receiver operating characteristic curve (ROC) was used to analyze the value of the levels of serum HMGB1, sCD40L, PIGF, and sFlt-1 for diagnosing and predicting PE. Results: The values of 24 h urinary protein, systolic blood pressure (SBP), diastolic blood pressure (DBP), and mean arterial pressure (MAP), and the levels of serum HMGB1, sCD40L, and SFLT-1 of the women in the study group were significantly higher than those of the women in the control group, but the serum PIGF level of the women in the study group was significantly lower. The variation range of the values of 24 h urinary protein, SBP, DBP, and MAP, and the levels of serum HMGB1, sCD40L, and SFLT-1 of the women in group B was significantly higher than those of the women in group A (all P＜0.05). ROC curve analysis had showed than the best value of ROC, the sensitivity, and the specificity of the combined the serum HMGB1, sCD40L, PIGF, and sFlt-1 levels for diagnosing PE were 0.810, 81.0%, and 82.1%, respectively. Conclusion: The levels of serum HMGB1, sCD40L, PIGF, sFlt-1 of the pregnant women are closely related to their occurrence of PE and their increased blood pressure. The combined detections of the serum HMGB1, sCD40L, PIGF, and sFlt-1 levels can predicate the occurrence and outcomes of the pregnant women with PE, which can provide reference basis for clinical diagnosis of PE.

2021 Vol. 29　(12):　2683- [Abstract]（ 287 ） HTML (0 KB)  PDF  (0 KB)  ( 35 )

To investigate the affecting factors of residual/recurrence of cervical intraepithelial neoplasia（CIN) after modified cold knife conization (CKC). Methods: The data from the medical records of 153 patients who underwent modified CKC from January 2019 to June 2020 were analyzed retrospectively, and all enrolled subjects had been followed up for 1 year. These patients were divided into two groups according to their postoperative residual/recurrence situation of CIN after modified CKC. The patients with residual/recurrence of CIN were in group A and the patients without residual/recurrence of CIN were in group B. Age, CIN involvement situation of cervical canal, CIN involvement quadrant of Cervix uteri, menopausal status, number of sexual partners, CIN glandular involvement status, CIN grade, preoperative human papillomavirus（HPV) infection, and postoperative HPV infection of the patients were compared between the two groups. Multifactorial logistic regression was used to analyze the factors influencing the residual/recurrence after CKC of CIN. Results: After the followed up for 1 year of 153 patients, there were 4 (2.6%) cases with postoperative residue of CIN, 12 (7.9%) cases with recurrence of CIN, and 137 (89.5%) cases without residue/recurrence of CIN. Univariate analysis showed that postoperative CIN residual/recurrence was associated with the age of the patients, and the situations of CIN cervical canal involvement, menopause, number of sexual partners, CIN involvement of cervical canal gland, CIN grade, preoperative and postoperative HPV infection of the patient (P＜0.05), but was not associated with the CIN involvement situation of cervical canal quadrant (P＞0.05). Conclusion: The corresponding preventive measures should be taken based on these independent risk factors of the residual/recurrence of CIN to improve the effect of modified CKC.

2021 Vol. 29　(12):　2692- [Abstract]（ 255 ） HTML (0 KB)  PDF  (0 KB)  ( 35 )

To explore the changes of the vaginal microecology of the women with vulvar intraepithelial neoplasia（VIN) and cervical squamous intraepithelial lesion（SIL), and to analyze the relationship between persistent human papilloma virus（HPV) infection of the women and their vaginal microecology. Methods: The clinical data of 90 women with high risk HP infection who had undergone colposcopy biopsy from February 2018 to October 2020 were collected retrospectively. These women were divided into group A (30 cases with VIN), group B (30 cases with SIL), and group C (30 cases with normal cervical biopsy) according to the results of pathological examination of these women. All these women were followed up for 6 months after treatment, and were further divided into group D（27 cases with persistent HPV infection) and group E（63 cases with HPV negative conversion) according to whether their HPV infection converted to negative. All these women had undergone vaginal microecological examination at the time of enrolling in this study, and the differences of vaginal microecology of the women with different diseases status were analyzed. The differences of the vaginal microecology of the women with persistent HPV infection and the women with HPV negative conversion were analyzed. Logistic regression was used to analyze the possibly factors affecting the persistent HPV infection of the women. Results: The incidences of trichomoniasis vaginitis (TV), bacterial vaginosis (BV) and lactobacillus reduction of the women in group A and group B were significantly higher than those of the women in group C. The persistent infection rate of HPV of the women in group A, in group B, and in group C after 6 months followed up were 43.3%, 36.7%, and 10.0%, respectively, which had decreased gradually (P＜0.05). The incidences of TV, BV, and lactobacillus reduction of the women in group D were significantly higher than those of the women in group E (P＜0.05). The rate of the BV and lactobacillus decreased of the women were the risk factors of their persistent infection of HPV (P＜0.05). Conclusion: The women with VIN and SIL all have vaginal microecological imbalance, and the incidence of their persistent HPV infection is higher. The women with persistent infection of HPV have the vaginal microecological imbalance status, which is association with their BV occurrence and their lactobacillus reduction.

2021 Vol. 29　(12):　2700- [Abstract]（ 326 ） HTML (0 KB)  PDF  (0 KB)  ( 34 )

To explore the influence of post-abortion care (PAC) services on the using and continue using of highly effective contraception role. Methods: From April 2019 to March 2020, 11250 women who had been given routinely PAC services from Wanjiang General Hospital were included in observation group, and the women who had not been given routinely PAC services from Puji Branch Hospital were included in control group. The implementation and continue using of post abortion highly efficiency contraception of the women were compared between the two groups. Results: The implementation rate of the highly effective contraception after induced abortion (95.0%, 1187/1250) of the women in the observation group was significantly higher than that (17.1%, 214/1250) of the women in the control group (P＜0.05). In the observation group, the implementation rate of highly effective contraception after induced abortion of the women with high-risk factors was 97.3% (569/585). The continua using rates of shortacting compound oral contraceptive (COC) of the women in the observation group in the 1st, 3rd, and 6th months of followed up were 98.9%, 69.4%, and 6.1%, respectively, and the continua using rates of short-acting COC of the women in the control group in the 1st, 3rd, and 6th months of followed up were 99.2%, 56.0%, and 42.0%, respectively. The continua using rate of COC of the women with high risk factors in the observation group in the 6th months after abortion was 88.2%. The implementation rate of intrauterine device (IUD) of the women in the observation group was 34.6%, which was significantly higher than that (7.5%) of the women in the control group (P＜0.05). The continua IUD using rate of the women in the observation group in the 1st, 3rd, and 6th month after abortion were 100%, 99.1%, and 99.4%, respectively, which had no significant different from those (100%, 100%, and 98%, respectively) of the women in the control group (P＞0.05). There were 53 women given IUD inserted in the 6th month after abortion in the observation group, which included 39 women with high risk factors. There were 17 women with IUD inserted in the 6th month after abortion in the control group. Conclusion: PAC service has good clinical effect for improving the immediate implementation and continua using of the highly effective contraception of the women after abortion.

2021 Vol. 29　(12):　2708- [Abstract]（ 423 ） HTML (0 KB)  PDF  (0 KB)  ( 26 )