To explore the value of serum soluble endothelial factor（sEng), placental protein 13（PP13), and peritoneal process guiding factor（Netrin-1) levels of the pregnant women during the first trimester of pregnancy for predicting their fetal growth restriction (FGR). Methods: The clinical data of 98 pregnant women with FGR in observation group and 98 pregnant women with normal fetal development in control group from January 2017 to January 2020 were collected retrospectively. The levels of sEng, PP13, and Netrin-1 of the women were compared between the two groups. The value of the levels of sEng, PP13, and Netrin-1 of the pregnant women for predicting their FGR was analyzed. Results: The sEng level of the women in the observation group was significantly higher than that of the women in the control group, but the levels of PP13 and Netrin-1 of the women in the observation group were significantly lower (P＜0.05). The sEng level of the women was not significantly correlated with their PP13 level (P＞0.05), the sEng level of the women was negatively correlated with their Netrin-1 level (P＜0.05), and the PP13 level of the women was positively correlated with their Netrin-1 level (P＜0.05). Receiver operating characteristic curve (ROC) analysis showed that the AUC of the sEng level, the PP13 level, the Netrin-1 level, and the combination of the detections of the sEng level, the PP13 level, and the Netrin-1 level of the pregnant women for predicting their FGR were 0.856, 0.903, 0.996, 1.000, respectively, and which of the Netrin-1 level and the combination of the sEng level, the PP13 level, and the Netrin-1 level were the highest (P＜0.05). According to the optimal critical value, when the sEng level≥36.735ng/L, the sensitivity and the specificity of the sEng level for predicting FGR were 71.4% and 92.9%, respectively. When the PP13 level ≤0.935ng/ml, the sensitivity and the specificity of the PP13 level for predicting FGR were 86.7% and 78.6%, respectively. When the Netrin-1 level ≤125.105 pg/ml, the sensitivity and the specificity of the Netrin-1 level for predicting FGR were 93.9% and 100%, respectively. The sensitivity and the specificity of the combination of the detections of the sEng level, the PP13 level, and the Netrin-1 level were for predicting FGR 100% and 100%, respectively. Conclusion: The serum sEng level of the pregnant women during the first trimester of pregnancy is negatively correlated with their Netrin-1 level, and the PP13 level of the pregnant women was positively correlated with their Netrin-1level. The serum sEng level, the serum PP13 level, and the serum Netrin-1 level have certain values for predicting FGR, and the value of the serum Netrin-1 level, and the combined serum sEng level, the serum PP13 level, and the serum Netrin-1 level for predictive predicting FGR are the highest.