To analyze the effect of transcutaneous electrical nerve stimulation (TENS) combined with Kangfuxiaoyan suppository for treating patients with chronic pelvic pain syndrome (CPPS). Methods: According to the principle of random number, 108 patients with CPPS were divided two groups from February 2017 to September 2020. The patients in the control group were treated with TENS, while the patients in the observation group were treated with TENS combined with Kangfuxiaoyan suppository. The values of ultrasonic index on musculus levator ani, the pain VAS score, and the different pain relief degree of the patients were compared between the two groups. Results: After treatment, the anterior and posterior diameter, the transverse diameter, and the area of the levator ani hiatus of the patients in the two groups had increased significantly, and which of the patients in the observation group was significantly higher than those of the patients in the control group. The VAS score of the patients in the two groups had decreased significantly, and which of the patients in the observation group was significantly lower than those of the patients in the control group (all P<0.05). There was no significant difference in the total pain relief rate of the patients between the two groups, but the relief degree of the patients in the observation group was significantly better than that of the patients in the control group (P<0.05). For the patients with VAS score ≤5 points before treatment, there were no significant differences in the anterior and posterior diameter, the transverse diameter, and the area of the levator ani hiatus of the patients between the two groups, and there were no significant differences in VAS score and relief degree of pain of the patients between the two groups (all P>0.05). For the patients with VAS score > 5 points before treatment, the anterior and posterior diameter, the transverse diameter, and the area of the levator ani hiatus of the patients in the observation group were significantly higher than those of the patients in the control group after treatment, while the VAS score of the patients in the observation group was significantly lower (P<0.05). Conclusion: The therapeutic effect of TENS combined with Kangfuxiaoyan suppository for treating patients with CPPS is better than that of TENS only, especially for those patients with severe pain symptoms.