Objective: To investigate the fertility intention and influencing factors of the population of childbearing age in Chengdu city, and provide evidence for improving population policy implement and promoting population healthy development. Methods: 875 people aged 15-49 years old from 11 districts or counties of Chengdu city were investigated by questionnaire self designed. The investigation included basic information, fertility intention, and the influencing factors of these people. Results: The ideal number of children of people was 2, but only 30.1% would intend to have the second child. 88.3% respondents more focused on children's health and education. In the weighted proportional ranking of the intention for the second child, the first rank was family happiness increased, and the second rank was one's family line continued. The family income was the first rank in the weighted proportional ranking of the influencing factors of fertility willingness. Conclusion: The willingness to have the second child of people in child-bearing age from Chengdu city is low, and there is different between their ideal number of children and the actual number of children. The people of childbearing age focus on the quality of children rather than the quantity of children. The purpose of reproduction is mainly influenced by personal emotion and traditional conception of reproduction. Economic income and child care are the main factors that affect fertility intention.

2020 Vol. 28　(3):　315- [Abstract]（ 422 ） HTML (0 KB)  PDF  (0 KB)  ( 43 )

Objective: To investigate the influence of diphereline combined with Danqi fukang oral paste for treating women with endometriosis on their levels of serum COX-2 Flk-1 and inflammatory factors. Methods: 100 women with endometriosis were randomly divided into contral group and study group (50 cases in each group) by the digital table method from March 2016 to March 2018. The women in the control group were treated by diphereline, and the women in the study group were treated by diphereline combined with Danshen fukang oral paste. The clinical efficacy, the levels of serum COX-2, Flk-1 and inflammatory factor, VAS pain score, and the rate of adverse reaction of women in the two groups were observed. Results: The effective rate of women in the study group was 94.0%, which was significant higher than that (66.0%) of women in the control group, but the serum levels of cox-2, flk-1, il-6 and il-8 of women in the study group were significant lower. After treatment,the VAS scores of women on the fifth day, the 20th day, and the 60th day were 4.3±1.4 points, 1.1±1.2 points, and 0.6±0.2 points, respectively, which were significant lower than those (7.0±1.3 points, 7.0±1.3 points, and 7.0±1.3 points, respectively)of women in the control group. The incidence of adverse reactions of women in the study group was 12.0%, which was significant lower than that (32.2%) of women in the control group (P<0.05). Conclusion: Diphereline combined with Danqi fukang oral paste for treating women with endometriosis can significantly increase the efficacy, and can reduce the levels of serum COX-2, Flk-1 and inflammatory factor, and the rate of adverse reaction.

2020 Vol. 28　(3):　324- [Abstract]（ 355 ） HTML (0 KB)  PDF  (0 KB)  ( 39 )

Objective: To study the influence of single-port laparoscopic surgery for treating women with ectopic pregnancy on their ovarian function, and the levels of human chorionic gonadotropin（beta-HCG) and estradiol（E2). Methods: 80 women with ectopic pregnancy were selected and were divided into two groups from May 2016 to May 2018.  42 women in the observation group were treated by single-port laparoscopic surgery and 38 women in the control group were given conventional laparoscopic surgery. The operative time, intraoperative blood loss, anal exhaust time, hospital stay time, the levels of AMH, beta HCG and E2, antra1 follicle count（AFC), and complications rate of women were compared between the two groups. Results: The operation time of women had no statistically significant different between the two groups (P>0.05). Intraoperative blood loss, anal exhaust time, and hospital stay time of women in the observation group were 67.5±5.4ml, 26.6±7.0h and 4.3±2.1d, respectively, which were significant lower than those of women in the control group (P<0.05). After treatment, the level of AMH and number of AFC of women in both groups had significantly decreased, but which had no significant difference between the two groups (P>0.05). After treatment, the levels of beta HCG and E2 of women in both groups had significantly increased, and the levels of beta HCG and E2 of women in the observation group were significant lower than those of women in the control group (P<0.05). The total incidence of complications of women in the observation group was 21.1%, which was significant higher than that (4.8%) of women in the control group (P<0.05). Conclusion: The effect of single-port laparoscopic surgery for treating women with ectopic pregnancy is better than that of conventional laparoscopic surgery, and single-port laparoscopic surgery has little impact on ovarian function of women.

2020 Vol. 28　(3):　332- [Abstract]（ 376 ） HTML (0 KB)  PDF  (0 KB)  ( 45 )

Objective: To investigate the expressions and significances of miR-223-3p and leukemia inhibitory factor (LIF) in endometrial tissue of infertile women with endometriosis. Methods: 22 infertile women with endometriosis were selected in study group from February 2017 to February 2018, and 25 married normal women with normal menstrual cycle were selected in control group. The endometrial tissue during the middle luteal phase of women in the two groups were collected. The expressions of miR-223-3p and LIF mRNA in endometrial tissue were detected by qRT-PCR, the expression of LIF protein in endometrial tissue was detected by immunohistochemistry, and the target gene of miR-223-3p was predicted by the microTarBase database, and Pearson correlation analysis was used to analyze the correlation between miR-223-3p expression and LIF level. Results: The expression of miR-223-3p of women in the study group was significant higher than that of women in the control group, and the expression of LIF of women in the study group was significant lower (P<0.05). The expression of LIF protein of women in the study group was 1.38±0.42, which was significant lower than that(3.65±0.81) of women in the control group (P<0.05). MicroTarBase database predicted that there were miR-223-3p binding sites on LIF gene. Pearson correlation analysis showed that miR-223-3p level of women in study group was negatively correlated with LIF level (r=-0.625, p=0.002). Conclusion: mir-223-3p expression in endometrial tissue of infertile women with endometriosis is high expression, but LIF expression is low, there is negatively correlated between the mir-223-3p expression and LIF expression. Mir-223-3p level may play a regulatory role in endometrial tissues by inhibiting the expression of LIF.

2020 Vol. 28　(3):　338- [Abstract]（ 341 ） HTML (0 KB)  PDF  (0 KB)  ( 39 )

Objective: To investigate the effect of ergometrine maleate combined with carbetocin for preventing postpartum hemorrhage of women with scar uterus pregnancy, and to study its influence on the blood coagulation function of women. Methods: 150 women with scar uterus pregnancy who wanted to cesarean section were selected and were divided into 3 groups according to the random number table method from January 30, 2016 to April 30, 2019 (50 cases in each group). The women in group A were given ergometrine maleate for preventing postpartum hemorrhage, the women in group B were given carbetocin for preventing postpartum hemorrhage, and the women in group C were given ergometrine maleate combined with carbetocin for preventing postpartum hemorrhage. The efficacy, intraoperative and postoperative bleeding volume, the change of uterine fundal height on the 1st, 3rd, and 5th days after cesarean section, the levels of blood coagulation parameters (D0dimer and Fibrinogen)before and after cesarean section, and the incidence of adverse reactions of women were compared among the three groups. Results: The total effective rate of women in group C was 96.0%, which was significant higher than that of women in group A and B (P<0.05). The 24h postoperative bleeding volume in group A was significant lower than that in group B (p<0.05). There was no significant different in the amount of intraoperative blood loss of women among the 3 groups (P>0.05). The postoperative bleeding volume in 24h of women in group C was the lowest, but that of women in group B was the highest. 24h after cesarean section, Hb value and fibrinogen level of women in group C were 112.7± 9.1g/L and 5.9±1.3), which were significant higher than those of women in group A and B, but the d-dimer level (3.1±0.8) of women in group C was significant lower (P<0.05). The degree of decreasing of uterine fundal height on the 1st, 3rd, and 5th days after cesarean section of women in group C was significant more than that of women in group A and B (P<0.05). The inci dence of adverse reactions of women in the three groups was 8.0%, 8.0%, and 10.0%, respectively, which had no significant difference (P>0.05). Conclusion: Ergometine maleate combined with carbetocin can effectively prevent intraoperative and postpartum hemorrhage of women with scar uterus pregnancy, and can promote uterine the decline of uterine fundal height, and can improve coagulation function of women.

2020 Vol. 28　(3):　345- [Abstract]（ 368 ） HTML (0 KB)  PDF  (0 KB)  ( 35 )

Objective: To study the analgesic effect of flurbiprofen axetil combined with fentanyl citrate in missed abortion. Methods: 160 women with missed abortion were selected and were divided into observation group and control group by random number table method from August 2018 to August 2019 (80 cases in each group). All included women in the two groups were treated with painless abortion. The women in the control group were anesthetized by fentanyl citrate combined with propofol before operation, and the women in the observation group were treated by flurbiprofen except to fentanyl citrate combined with propofol. After abortion, the visual analogue scale (VAS) of women 6, 12, 24 and 36 hours after operation, the perioperative mean arterial blood pressure (MAP), heart rate (HR), systolic blood pressure (SBP), diastolic blood pressure (DBP), operation time, recovery time, and propofol dosage of women were compared between the two groups. The incidence of adverse reactions during anesthesia of women in both groups was counted. Results: The VAS score of women in the observation group at different time points after abortion was significant lower than that of the women in the control group (P<0.05). There were no different in the values of MAP, HR, SBP, and DBP of all women before and after abortion, and there were also no different in the values of MAP, HR, SBP, and DBP of women between the two groups (P>0.05). The postoperative wake time and the dosage of propofol of women in the observation group were 3.53±1.61min and 2.21±1.27 mg/kg respectively, which were significant less than those of women in the control group, but the overall incidence of adverse reactions of women in the observation group was 5.0%, which was significant lower than that (16.3%) of women in the control group (P<0.05). Conclusion: Flurbiprofen axetil combined with fentanyl citrate used in missed abortion can achieve better analgesic effect, which can reduce the dosage using of propofol, shorten the recovery time after operation, and reduce the incidence of adverse drug reactions.

2020 Vol. 28　(3):　353- [Abstract]（ 297 ） HTML (0 KB)  PDF  (0 KB)  ( 41 )

Objective: To analyze the value of serum placental growth factor (PLGF), lactate dehydrogenase, and free estriol (uE3) levels for predicting placental abruption of pregnant women with late-onset preeclampsia. Methods: The clinical data of 265 pregnant with late-onset preeclampsia were analyzed retrospectively, and all included women were followed up to delivery, which included 47 women with placental abruption occurred in group A and 218 women without placental abruption in group B. The clinical data and serum indexes of women were compared between the two groups. Logistic regression was used to analyze the risk factors of placental abruption of pregnant women with preeclampsia. The ROC curve was used to evaluate the value of the serum indexes for diagnosing placental abruption of pregnant women with preeclampsia. Results: The average gestational weeks when delivery, serum PLGF and uE3 levels of women in group A were significant lower than those of women in group B, but the serum creatinine and lactate dehydrogenase levels, the proportions of childbirth history and oligohydramnios of women in group A were significant higher (P<0.05). Logistics multivariate analysis showed that the levels of PLGF, lactate dehydrogenase and uE3, childbirth history, and oligohydramnios were independent risk factors of placental abruption of women with preeclampsia (P<0.05). The area under the ROC of serum PLGF level combined with lactate dehydrogenase level and uE3 level was significant greater than that of serum PLGF level, lactate dehydrogenase level, or uE3 level alone (P=0.019, 0.002, 0.001). The sensitivity and the specificity of serum PLGF level combined with lactate dehydrogenase level and uE3 level were significant better than that of serum PLGF level, lactate dehydrogenase level, or uE3 level alone (P<0.05). Conclusion: The levels of serum PLGF, lactate dehydrogenase, and uE3 have certain value predicting placental abruption of pregnant women with late-onset preeclampsia. The serum PLGF level combined with lactate dehydrogenase level and uE3 level can improve the diagnostic efficiency.

2020 Vol. 28　(3):　362- [Abstract]（ 316 ） HTML (0 KB)  PDF  (0 KB)  ( 42 )

Objective: To explore the influencing factors of vaginal trial of labor after previous cesarean delivery (TOLAC), and to study the pregnancy outcomes after different delivery modes. Methods: From February 2017 to September 2018, 200 pregnant women with scar uterus were divided into two groups according to the prospective cohort study criteria and the desire to give birth of these women. The 63 women in group B were given cesarean delivery, and 137 women in the group A were given TOLAC, which included 114 women with successful TOLAC and 23 women with TOLAC failure. Pregnancy outcomes and neonatal status in each group were recorded, and logistic multivariate analysis was used to analyze the influencing factors of TOLAC. Results: In group A, 114 (83.2%) women were successful TOLAC, and 23 women were TOLAC failure, which included 18 women given up vaginal trial labor due to intense pain during labor, 2 women with persistent posterior occipital position, one woman with tenderness of the lower uterine segment and pain of lower abdomen intensified during uterine contraction, and 2 women because of amniotic fluid Ⅲ degree meconium contamination. In group A, maternal age, BMI, in hospital about to give birth, history of natural labor, and cervix uteri dilatation to be hospital were independent factors for influencing the success of TOLAC. There were no difference in puerperal time, postpartum hemorrhage rate, intrapartum weakness rate, fetal distress rate, neonatal asphyxia rate, and neonatal birth weight between group A and group B (P>0.05), but neonatal Apgar score at 1min after born in group A was significant higher than that in group B (P<0.05). Conclusion: The women > 30 years old and BMI > 30kg/m2 can increase the risk of TOLAC failure. The pregnant women with a history of natural delivery, or their cervix uteri dilatation well when admits to hospital, or to be hospital immediately when about to give birth can improve the success rate of TOLAC. TOLAC does not lead to adverse pregnancy outcomes, but the indications of TOLAC still should be controlled strictly to ensure the safety of women who wanted for TOLAC.

2020 Vol. 28　(3):　370- [Abstract]（ 299 ） HTML (0 KB)  PDF  (0 KB)  ( 39 )

Objective: To study the effect of different laparoscopic surgeries for treating women with ovarian mature teratoma, and to analyze its influence on their ovarian reserve function. Methods: 64 women with ovarian mature teratoma were selected and were divided into study group and control group by random number method (32 cases in each group) from October 2015 to June 2017. The women in the study group were treated with two-hole laparoscopic surgery, while the women in the control group were treated with three-hole laparoscopic surgery. The operative indexes, the postoperative curative effect, and the incidence of complications of women in the two groups were analyzed. The levels of basic follicle stimulating hormone (FSH), estradiol (E2), and luteinizing hormone (LH) were measured by radioimmunoassay, the levels of antimullerian hormone (AMH) and inhibin B (INHB) were determined by ELISA, and the surgical ovarian volume and antral follicle count (AFC) were measured by ultrasonography. Results: The amount of intraoperative blood loss, time to bed and hospital stay of women in the study group were significant lower than those of women in the control group (P<0.05). One year after the operation, the total effective rate of women in the study group was 93.8%, which was significant higher than that (71.9%) of women in the control group, but the complication rate of women in the study group was 6.3%, which was significant lower than that (25.0%) of women in the control group. After operation, the levels of FSH, E2, LH, and AFC, and ovarian volume of women in the two groups had decreased significantly, and the levels of FSH, E2, LH of women in the study group were significant lower than those of women in the control group, but ovarian volume of women in the study group was significant higher. After operation, the levels of AMH and INHB of women in the study group were significant higher than those of women in the control group (P<0.05). Conclusion: Two-hole laparoscopic surgery for treating women with ovarian mature teratoma has a better therapeutic effect, which can reduce the incidence of postoperative complications and improve the recovery of ovarian reserve function.

2020 Vol. 28　(3):　378- [Abstract]（ 310 ） HTML (0 KB)  PDF  (0 KB)  ( 35 )

Objective: To investigate the clinical effect of microdissection of testicular sperm extraction (micro-TESE) for treating patients with non-obstructive azoospermia (NOA). Methods: The clinical data of 60 patients with NOA treated by micro-TESE (in group A) and 48 patients with obstructive azoospermia (OA) treated by testicular puncture (in group B) from July 2017 to December 2018 were analyzed retrospectively. The wives of the patients in the two groups received IVF-ET by intracytoplasmic sperm injection (ICSI). The effect of assisted pregnancy, changes of sex hormone levels of patients before and after NOA treatment were compared between the two groups. Results: The levels of FSH and LH of patients in group A were significant higher than those of patients in group B, but T level and sperm acquisition rate (SRR) of the patients in group A were significant lower than those of patients in group B (P<0.05). In 1 month and 6 months after operation, the levels of FSH and LH of patients in the two groups had no significant different (P>0.05), but T level and IIEF-5 scores had decreased significantly (P<0.05). Conclusion: Micro-TESE can provide opportunity for some patients with NOA to have their own biological offspring, which has high success rate of sperm extraction without obvious complications, but it may damage the testicular function of patients to some extent.

2020 Vol. 28　(3):　385- [Abstract]（ 329 ） HTML (0 KB)  PDF  (0 KB)  ( 41 )

Objective: To explore the influence of basal FSH (bFSH) as progestin primed ovarian stimulation (PPOS) protocol for treating women with diminished ovarian reserve (DOR) on their outcomes of in vitro fertilization (IVF) or intracytoplasmic sperm injection (ICSI). Methods: The data of 328 women with DOR under PPOS from June 2016 to November 2017 were analyzed retrospectively, and these women were divided into Group A (women were given bFSH <10 IU/L) and Group B (women were given bFSH≥10 IU/L) according to the dosage of bFSH used. The baseline information, ovarian stimulation characteristics, and laboratory outcome of embryo of women in the two groups were collected and analyzed. Results：The value of bLH of women in group B was 5.0±1.8, which was significant higher than that (2.5±1.5) of women in group A (P<0.001), and the value of bE2 of women in group B 168.4±183.4, which was significant lower than that (231.0±228.8) of women in group A (P<0.01). The numbers of follicle >14mm and oocytes of women in group B on hCG day were 2.8±1.7 and 3.0±2.1, which were significant lower than those (3.4±2.1 and 3.9±2.7) of women in group A (P<0.05). However, there were no significant different in the mature egg rate, 2PN fertilization rate, 2PN cleavage rate, high-quality embryo rate, transplantable embryo rate, high-quality embryo number, and utilized embryo number of women between the two groups (P> 0.05). There was no difference in clinical pregnancy rate in frozen embryo transfer of women between the two groups (P>0.05). Conclusion：PPOS protocol is benefit for women with DOR, especially for those women with high bFSH, which has potential application value.

2020 Vol. 28　(3):　394- [Abstract]（ 304 ） HTML (0 KB)  PDF  (0 KB)  ( 42 )

Objective: To analyze the routine causes of early recurrent spontaneous abortion of women, and to study the chromosomal abnormalities of its aborted embryos. Methods: The clinical data of 104 women with early recurrent spontaneous abortion were analyzed retrospectively. All included women underwent pelvic B ultrasonography, hysteroscopy, and embryo chromosome examination. The etiology of early recurrent spontaneous abortion and the abnormal karyotype distribution of embryos of women were analyzed. Results: The causes of miscarriage included chromosomal abnormalities of the husband and wife (7.7%), uterine abnormalities (8.8%), endocrine abnormalities of women (13.7%), and chromosomal abnormalities of the aborted embryo (59.8%). The age of women with ≥3 times abortion was significant higher than that of women with 2 times abortion (P<0.05), while there was no different in body mass index of women, rate of chromosomal abnormality of the husband and wife, and rates of uterine and endocrine abnormality between women with ≥3 times abortion and women with 2 times abortion (P>0.05). The chromosomal abnormality rate of embryos of women with 2 times abortion was significant higher than that of women with ≥3 times abortion (P<0.05). 68 (61.8%) in 110 aborted embryos after chromosome detection had karyotype abnormalities, which included 53 cases with aneuploidy of chromosome, 5 cases with polyploidy of chromosome, and 10 cases with other chromosomal abnormalities. In the aborted embryos with aneuploidy of chromosome, 16-trisomy (19.1%), 22-trisomy (10.3%) and X monomer (10.3%) were the first three types of chromosomal abnormalities. Conclusion: The rate of embryo chromosome abnormalities of women with early recurrent spontaneous abortion are high, which should be screened as a common cause of recurrent abortion, and it is more reasonable to screen the etiology of women after two times spontaneous abortions.

2020 Vol. 28　(3):　404- [Abstract]（ 314 ） HTML (0 KB)  PDF  (0 KB)  ( 38 )

Objective: To explore the value of blood flow spectrum for predicting the pregnancy outcomes of women with early-onset preeclampsia. Methods: The clinical data of 120 women with early-onset pre-eclampsia (in study group) and 100 pregnant women without pre-eclampsia (in control group) were collected retrospectively. The changes of blood flow spectrum of uterine artery and umbilical artery of women in the two groups were examined by three-dimensional color Doppler ultrasound during the third trimester of pregnancy. The women in the study group were also divided into group A (with infant adverse outcomes), group B (with maternal adverse outcomes), and group C (without adverse pregnancy outcomes) according to the pregnancy outcomes, and the blood flow spectrum of women was compared among the four groups. The receiver operating characteristic (ROC) curve was used to analyze the efficacy value of the blood flow spectrum for predicting adverse pregnancy outcomes. Results: The S/D, PI and RI values of the uterine artery of women in the study group were significant higher than those of women in the control group, the incidences of perinatal infant and maternal adverse outcomes were 28.3% and 21.7%, which were significant higher than those (5.0% and 4.0%) of women in the control group (P<0.05). In the study group, the S/D, PI and RI values of uterine artery and umbilical artery of women with adverse pregnancy outcomes were significant higher than those of women without adverse pregnancy outcomes (P<0.05). The S/D, PI and RI values of uterine artery and umbilical artery of women with early-onset preeclampsia could predicted the adverse pregnancy outcomes effectively, which sensitivity and specificity were 75%-80% and of 83%-86%. Conclusion: There is a close relationship between the changes of blood flow spectrum of uterine artery and umbilical artery of women with earlyonset preeclampsia and their maternal and infant prognosis. Monitoring the blood flow spectrum of uterine artery and umbilical artery can provide an effective evidence for predicting maternal and infant adverse outcomes.

2020 Vol. 28　(3):　412- [Abstract]（ 296 ） HTML (0 KB)  PDF  (0 KB)  ( 35 )

Objective: To analyze the influence of clinical features during the previous pre-eclampsia of pregnant women on their pre-eclampsia recurrence rate and the gestational weeks of pre-eclampsia onset again. Methods: The clinical data of 155 pregnant women with a history of pre-eclampsia from January 2016 to December 2018 were collected retrospectively, and were divided in group A (women with pre-eclampsia again) and group B (women without pre-eclampsia again). The influence of clinical features during last pre-eclampsia of pregnant women on their pre-eclampsia recurrence rate and the different gestational weeks (＜34 and ≥34 gestational weeks) of pre-eclampsia onset again was analyzed. Results: There were statistically significant different in proprotions of the previous preeclampsia onset ≤28 gestational weeks, pregnancy interval ≥5 years, systolic blood pressure during pregnancy ≥160mmHg, diastolic blood pressure ≥110mmHg, and urine protein ≥2.0g/24h of women between group A and group B (P<0.05), but there were no difference in the gestational weeks for pregnancy termination of the previous pregnancy and the rate of serious complications of women between the two groups (P>0.05). Pre-eclampsia onset ≤28 gestational weeks, systolic blood pressure ≥160mmHg, diastolic blood pressure ≥110mmHg during the previous pregnancy were independent risk factors of recurrent preeclampsia (P< 0.05). Pre-eclampsia onset in 28-34 gestational weeks, pregnancy interval ≥5 years, and urine protein ≥2.0g/24h had no correlation with recurrent preeclampsia (P>0.05). There were statistically significant different in proprotions of the previous pre-eclampsia onset ≤28 gestational weeks, systolic blood pressure ≥160mmHg and diastolic blood pressure ≥110mmHg during last pregnancy between women with pre-eclampsia onset <34 gestational weeks and women with pre-eclampsia onset ≥34 gestational weeks (P<0.05), but there were no difference in the gestational weeks of pregnancy termination of last pregnancy, pregnancy interval ≥5 years, urine protein ≥2.0g/24h, and severe complications rate of women between the two groups (P>0.05). Conclusion: The early onset pre-eclampsia and severe hypertension occurrence during the previous pregnancy are the independent risk factors for the recurrence of pre-eclampsia, and also affect gestational weeks of pre-eclampsia onset again.

2020 Vol. 28　(3):　420- [Abstract]（ 289 ） HTML (0 KB)  PDF  (0 KB)  ( 36 )

Objective: To investigate the relationship between the tissue inhibitor of metalloproteinase -2 (TIMP-2) and granulocyte colony stimulating factor (G-CSF) levels and chorioamnionitis of women with premature rupture of membranes. Methods: 83 women with premature rupture of membranes were selected i observation group, and 83 normal healthy pregnant women with full-term delivery were included in control group from March 2017 to February 2019. The levels of TIMP-2 and G-CSF in the embryolemma tissue of women in the two groups were detected. According to the results of laboratory indications and clinical examination, the pregnant women in the observation group were further divided into 3 groups, which included group A (24 women without chorioamnionitis), group B (29 women with laboratory indications and without clinical symptoms), and group C (30 women with chorioamnionitis). The levels of TIMP-2 and G-CSF of women were compared among the four groups, and the relationship between TIMP-2 and G-CSF levels and the severity degree of chorioamnionitis of women were analyzed. Results: The expression level of TIMP-2 of women in the observation group was significant higher than that of women in the control group, but the level of G-CSF of women in the observation group was significant lower (P<0.05). In the observation group, the level of TIMP-2 in group A was the highest, but the level of TIMP-2 was the lowest. The TIMP-2 level was negative ly correlated with the rate of chorioamnionitis of women with premature rupture of membranes (r=-0.647, P<0.05), but the G-CSF level was positively correlated with the rate of chorioamnionitis of women with premature rupture of membranes (r=0.657, P<0.05). Conclusion: TIMP-2 and G-CSF levels in embryolemma tissue of women with premature rupture of membranes detection maybe provide evidence for differential diagnosing chorioamnionitis.

2020 Vol. 28　(3):　428- [Abstract]（ 260 ） HTML (0 KB)  PDF  (0 KB)  ( 36 )

Objective: To study the relationship between serum micro-RNA-26b (miR-26b) expression of women with polycystic ovarian syndrome (PCOS) and their glycolipid metabolism. Methods: 80 women with PCOS were selected into observation group from March 2016 to January 2019, and another 80 infertility women without PCOS were included in control group during the same time. The expression of serum miR-26b and indexes of women were compared between the two groups, and the correlation between the expression of serum miR-26b and the glycolipid metabolism indexes levels was analyzed. The expression of serum miR-26b of women in the observation group was analyzed by multiple stepwise regression analysis. Results: The levels of fasting blood glucose(FBG), triglyceride(TG), total cholesterol(TC), low density lipoprotein cholesterol(LDL-C), fasting insulin (FINS), and miR-26b, and insulin resistance index (HOMA-IR) value of women in the observation group were significant higher than those of women in the control group (P<0.05). Pearson method had showed that the expression of serum miR-26b of women in the observation group was positively correlated with their LDL-C level and values of HOMA-IR and BMI (P<0.05). Multiple stepwise regression analysis showed that HOMA-IR was a risk factor of the serum miR-26b expression of women in the observation group (OR>1). Conclusion: The expression of serum miR-26b of women with PCOS is closely related to their glucolipid metabolism, and HOMA-IR is a risk factor of their serum miR-26b expression. As a monitoring target in clinical practice, serum miR-26b level can provide evidence for clinical diagnosis and treatment of women with PCOS.

2020 Vol. 28　(3):　435- [Abstract]（ 299 ） HTML (0 KB)  PDF  (0 KB)  ( 39 )

Objective: To explore the detection rate of immune antibodies of infertility women, and to study its related risk factors. Methods: 61 infertile women were enrolled in study group from January 2018 to October 2018, and 61 healthy women with normal birth history were selected in control group. The detection rates of anti-β2-glycoprotein I (β2-GPI）antibody, anti-thyroid peroxidase antibody (A-TPOAb), anti-sperm antibody (AsAb), and endometrial antibody (EMAb) of women were compared between the two groups. And the related risk factors of infertility were analyzed. Results: In the study group, 18 (29.51%) women were primary infertility, 43 (70.49%) women were secondary infertility. The detection rates of anti-β2-GPI, A-TPOAb, AsAb, and EMAb of women in the study group were 24.6%, 19.7%, 23.0%, and 29.5%, respectively, which were significant higher than those of women in the control group (P<0.05). In the study group, the detection rates of anti-β2-GPI, AsAb, and EMAb of women with primary infertility were 16.4%, 14.8%, and 19.7%, respectively, which were significant higher than those of women with secondary infertility (P<0.05), but the detection rate of A-TPOAb of women with primary infertility had no different to that of women with secondary infertility. In the study group, there were 41.0% women with ovulation disorders, 29.5% women with immune factors, 21.3% women with pelvic inflammatory disease, and 19.7% women with fallopian tube lesions, which all were the main risk factors of infertility. Conclusion: The levels of anti-β2-GPI, A-TPOAb, AsAb, and EMAb of women has a close relationship with infertility, which has important significance for the diagnosiing immune infertility. The ovulation disorders, immune factors, pelvic inflammatory disease, and fallopian tube lesions are the main factors leading to infertility, so they should be paid more attention to in clinical practice.

2020 Vol. 28　(3):　443- [Abstract]（ 341 ） HTML (0 KB)  PDF  (0 KB)  ( 42 )

Objective: To investigate the influence of early syphilis diagnose and intervention of pregnant women on their pregnancy outcomes and neonatal health. Methods: The clinical data of 154 pregnant women with syphilis from August 2017 to June 2018 were analyzed retrospectively, in which, 49 women in group A were given treatment of syphilis during the first trimester pregnancy, 43 women in group B were given treatment of syphilis during the third trimester pregnancy, and 62 women in group C were not given treatment of syphilis. The pregnancy outcomes and newborn health status of women were compared among the three groups. Results: The full-term newborn rate and survival rate of women in group C were 16.1% and 51.6%, which were significant lower than those of women in group A and B, but the incidences of premature delivery and aborted stillbirth of women in group C were 35.5% and 48.5%, which were significant higher than those of women in group A and B (P<0.05). The incidence of abortion and stillbirth of women in group A was 0.0%, which was significant lower than that (9.3%) of women in group B (P<0.05). Among the 120 newborns, there were 43 (35.8%) newborns with congenital syphilis, which included 3 cases (6.1%) in group A, 13 cases (33.3%) in group B, and 32 cases (84.4%) in group C, and there were significant different in the rate of newborns with congenital syphilis among the three groups (P<0.05). Before delivery, the proportions of normal newborns and newborns with congenital syphilis of women with the TRUST titer ≤ 1:4 were 79.1% and 50.0%, which were significant higher than those (13.2% and 42.7%) of women with the TRUST titer ≥ 1:8 (P<0.05). The average Apgar score of 1 min and 5 min of newborns after born in group A were 8.38±0.40 scores and 8.47±0.36 scores, which were significant higher than those of newborns (8.04±0.31 scores and 8.14±0.32 scores) in group B, and those of newborns in group A and B were significant higher than those of newborns (7.74±0.27 scores and 7.86±0.28 scores) in group C (P<0.05). Conclusion: Early syphilis diagnose and intervention of pregnant women can effectively improve the their pregnancy outcomes, and the treatment effect of syphilis during the first trimester pregnancy is better than that during the second and third trimester pregnancy.

2020 Vol. 28　(3):　449- [Abstract]（ 330 ） HTML (0 KB)  PDF  (0 KB)  ( 35 )