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| Expressions and significances of MiR-223-3p and leukemia inhibitory factor in endometrial tissue of infertile women with endometriosis |
| Xianning Central Hospital,The First Affiliated Hospital of Hubei University of Science And Technology,437000 |
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Abstract Objective: To investigate the expressions and significances of miR-223-3p and leukemia inhibitory factor (LIF) in endometrial tissue of infertile women with endometriosis. Methods: 22 infertile women with endometriosis were selected in study group from February 2017 to February 2018, and 25 married normal women with normal menstrual cycle were selected in control group. The endometrial tissue during the middle luteal phase of women in the two groups were collected. The expressions of miR-223-3p and LIF mRNA in endometrial tissue were detected by qRT-PCR, the expression of LIF protein in endometrial tissue was detected by immunohistochemistry, and the target gene of miR-223-3p was predicted by the microTarBase database, and Pearson correlation analysis was used to analyze the correlation between miR-223-3p expression and LIF level. Results: The expression of miR-223-3p of women in the study group was significant higher than that of women in the control group, and the expression of LIF of women in the study group was significant lower (P<0.05). The expression of LIF protein of women in the study group was 1.38±0.42, which was significant lower than that(3.65±0.81) of women in the control group (P<0.05). MicroTarBase database predicted that there were miR-223-3p binding sites on LIF gene. Pearson correlation analysis showed that miR-223-3p level of women in study group was negatively correlated with LIF level (r=-0.625, p=0.002). Conclusion: mir-223-3p expression in endometrial tissue of infertile women with endometriosis is high expression, but LIF expression is low, there is negatively correlated between the mir-223-3p expression and LIF expression. Mir-223-3p level may play a regulatory role in endometrial tissues by inhibiting the expression of LIF.
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