|
|
|
| Expression of HIF-1α, VEGF and sFlt-1 mRNA in placenta tissue of patients with preeclampsia and their clinical significance |
| The Sixth People's Hospital of Huizhou, Guangdong Province, 516211 |
|
|
|
|
Abstract Objective: To investigate the expression of hypoxia inducible factor-1 alpha (HIF-1 alpha), target gene vascular endothelial growth factor (VEGF), soluble vascular endothelial growth factor receptor-1 (sFlt-1) in placenta tissue of patients with preeclampsia, and to study their clinical significance. Methods: 120 pregnant women with preeclampsia were selected in observation group and 100 normal pregnant women were selected in control group from June 2016 to June 2018. Immunohistochemical streptomyces antibiotic proteinperoxidase ligation (SP) was used to detect the expression levels of HIF-1a, VEGF and sFlt-1. RT-PCR was used to detect the changes of HIF-1a, VEGF and sFlt-1 in placenta tissues of pregnant women in both groups, and the correlation among them was analyzed. Results: The expression rate of HIF-1 alpha protein (+++) and sflt-1 protein (+++) of women in the observation group were 45.0% and 52.5%, which were significant higher than those (12.0% and 20.0%) of women in the control group, but the expression rate of VEGF protein (+++) was 14.2%, which was significant lower than that (47.0%) of women in the control group (P<0.05). There was no significant different in VEGF mRNA expression level of women between the two groups (P>0.05). The levels of Hif-1 mRNA and sflt-1 mRNA of women in the observation group were significant higher than those of women in the control group, while the value of VEGFm RNA/ sflt-1 mRNA of women in the observation group was significant lower (P<0.05). In the observation group, the hif-1 mRNA level of women was positively correlated with the sflt-1 mRNA level(r=0.578, P<0.05), but was negatively correlated with the value of VEGF mRNA/ sflt-1 mRNA (r=-0.382, P<0.05). Conclusion: The changes of HIF-1α, VEGF and sFlt-1 levels maybe correlated with the pathophysiological of women with preeclampsia, which may play a role in the occurrence and development of preeclampsia.
|
|
|
|
|
|
|
|
|
