To investigate the relationship between wildtype p53 induced phosphatase 1(Wip1) and the invasion of ovarian cancer SKOV3 cells, and to explore the effect of Wip1 on the invasion and migration of SKOV3 cells. Methods: Scratch test and transwell chamber method were used to determine the invasion of SKOV3 cells in vitro after Wip1 silencing. The expressions of MMP-2, MMP-9, TIMP-1 and TIMP-2mRNA of SKOV3 cells were detected by real-time fluorescent quantitative reverse transcription-polymerase chain reaction(RT-PCR).The expression of matrix metalloproteinases2(MMP-2), MMP-9, tissue inhibitors of metalloproteinases (TIMP-1) and TIMP-2 protein of SKOV3 cells were determined by Western blot. Results: The cell migration distance in Wip1-siRNA group was significant shorter than that in control group (P<0.01). The number of SKOV3 cells migrated into the transwell chamber membrane in Wip1-siRNA group was significant less than that in control group (P<0.01).When compared with control group, significant reduction was seen in the expression of MMP-2 and MMP-9 in Wip1-siRNA group (P<0.01). Conclusion: Silencing Wip1 can inhibit the invasion of human ovarian cancer SKOV3 cells, which possibly do that by inhibiting the expression of MMP-2 and MMP-9.

2019 Vol. 27　(4):　417- [Abstract]（ 503 ） HTML (0 KB)  PDF  (0 KB)  ( 35 )

To investigate the status of reproductive health of the left behind children from the rural areas of Yun'nan and Hebei Provinces. Methods: Stratified cluster sampling method was used to select 205 eligible participants as the target population. Epidemiological survey using self-designed questionnaire was conducted. All the analyses were done by SPSS software (version 13.0).Results: The proportion of grandparents' guardianship for Left behind pupils' was high (>90.0%). Though they had a little knowledge of reproductive health (for example, more than 80.0% of them knew and had a sense of body privacy protection), their knowledge of reproductive health was still scarce (about 80.0% of them did not know "menstruation " and 50.0% of them did not know the behavior leading to pregnancy). Half of them could not or were not willing to talk about the reproductive problems with their parents and the other elders. Most of them were lack of health awareness when they faced to possible adverse sexual behavior occurs. For unplanned pregnancy, only 2/3 of the participants from Yun'nan and 14.0% of those from Hebei province would visit a doctor. Conclusion: In order to improve the reproductive health of the left behind children from Chinese rural areas, some special strategy and methods such as reproductive health education for the youth based on multi sectoral participation, school focused on, and family members involved in, should be performed immediately.

2019 Vol. 27　(4):　428- [Abstract]（ 372 ） HTML (0 KB)  PDF  (0 KB)  ( 37 )

To investigate the expressions of stem cell factor receptor (c-kit) and phosphotyl inosital 3 kinase (PI3K) in uterine scar tissue, and to study their relationships with uterine scar healing. Methods: 110 pregnant women underwent cesarean sections again were selected into the study group, and 25 pregnant women underwent cesarean section at the first time were selected into control group. The women in study group were also divided into group A (87 cases with healed well of uterine scar) and group B (23 cases with undesirable healing of uterine scar) according to the condition of uterine scar healing during cesarean section again. Real time fluorescent quantitative PCR (qRT-PCR) and immunohistochemistry were used to detect the relative expressions of c-kit and PI3K of all included women. The influencing factors of uterine scar healing were analyzed by Logistic analysis. Results: The expressions and the levels of of c-kit and PI3K of women with history cesarean section <3 years or≥8 years were significant higher than those of women in control group (P<0.05). The rates and levels of positive expressions of c-kit and PI3K expressions of women with undesirable healing of uterine scar after cesarean section after operation 3-4 years were the lowest (P<0.05). And the levels of positive expressions of c-kit and PI3K of women with undesirable healing of uterine scar were significant higher than those of women with healed well of uterine scar (P<0.05). Logistic analysis showed that the levels of c-kit and PI3K were risk factors for uterine scar healing. Conclusion: The expression levels of c-kit and PI3K in uterine scar tissue can promote uterine scar healing, but their over expressions are not benefit for uterine scar healing, 3-4 years after cesarean section is the best period for uterine scar healing.

2019 Vol. 27　(4):　437- [Abstract]（ 274 ） HTML (0 KB)  PDF  (0 KB)  ( 34 )

To investigate the influence of regular intermittent epidural injection combined with dexmedetomidine on effect of labor analgesia and the levels of peripheral blood fibrinogen and D-dimer of postpartum women. Methods: 126 single pregnant women with full-term were selected as subjects from January 2016 to February 2018, and these women were divided into study group control group (63 cases in each group) by random number table method. The women in study group were treated by dexamethasone combined with regular intermittent epidural injection, and the women in control group were treated by regular intermittent epidural injection only. The analgesic afficacy of women in the two groups were evaluated, and the influence on the indicators of coagulation function ［such as prothrombin time (PT), partial activated thromboplastin time (APTT), thrombin time (TT), fibrinogen, and D-dimer］ were analyzed. Results: The effective time, onset time, maintenance time, the second stage and third stage time of labor of women in study group were significant shorter than those of women in control group (P<0.05), but the time of keeping analgesia and total analgesia were significant longer.The amount of extramembranous used of women in study group was significant less than that of women in control group (P<0.05). The PT, APTT and TT of women in study group were significant longer than those of women in control group (P<0.05), but the levels of FIB and D-D were significant lower than those of women in control group (P<0.05). There was no difference in the labor incubation period of women between the two groups (P>0.05). Conclusion: Dexmedetomidine combined with regular intermittent epidural injection intravenous injection can reduce rate of postpartum hypercoagulability, reduce the amount of epidural anesthetic drugs, shorten the labor time significantly, prolong analgesic time, and shorten the onset time, so it can be used in clinical practice.

2019 Vol. 27　(4):　446- [Abstract]（ 347 ） HTML (0 KB)  PDF  (0 KB)  ( 46 )

To observe the effect of pelvic floor rehabilitation training combined with bioelectrical stimulation for treating parturients with pelvic floor dysfunction after different delivery modes. Methods: From September 2016 to May 2018, 206 parturients with pelvic floor dysfunction were divided into group A1 (n=54, underwent vaginal delivery and pelvic floor rehabilitation training), group A2 (n=50,underwent vaginal delivery and pelvic floor rehabilitation training combined with bioelectrical stimulation), group B1 (n=50, underwent cesarean section and pelvic floor rehabilitation training), and group B2 (n=52, underwent cesarean section and pelvic floor rehabilitation training combined with bioelectrical stimulation) according to different delivery modes and treatment method. The clinical efficacy of parturients was compared among the four groups. Results: After treatment, the total effective rate of parturients in group A2 and group B2 was significant higher than that of parturients in group A1 and group B1 (P<0.05). The total electromyographic value, class I and class II muscle fiber electromyographic values, and class II muscle fiber fatigue degrees of all parturients were significant better than those of parturients before treatment (P<0.05), and those of parturients in group A2 were the best (P<0.05), but there was no significant difference in class II muscle fiber fatigue degrees of parturients between before and after treatment (P>0.05). The vaginal rest pressure and systolic blood pressure of parturients in group A1 and group A2 were significant lower than those of parturients in group B1 and group B2 (P<0.05),and and those of parturients in group A2 were the lowest (P<0.05). Conclusion: The pelvic floor dysfunction degree of parturients underwent vaginal delivery is worse than that of parturients underwent cesarean section women. The pelvic floor rehabilitation training combined with bioelectrical stimulation for the treating parturients with pelvic floor dysfunction after different delivery modes is better than that of parturients treated by pelvic floor rehabilitation training alone. Moreover, after treatment, the situation of parturients underwent vaginal delivery is better than that of parturients underwent cesarean section.

2019 Vol. 27　(4):　453- [Abstract]（ 421 ） HTML (0 KB)  PDF  (0 KB)  ( 41 )

To observe the effect of timely subcutaneous implant and intrauterine device (IUD) used immediately after induced abortion. Methods: 320 women with artificial abortion and contraceptive demand were selected as research subjects form February 2014 to February 2016, and divided into group A(160 women had received subcutaneous implantation immediately after induced abortion) and group B (160 women had received IUD containing copper inserted immediately after induced abortion). The rates of pregnancy, termination due to disease, and adverse reactions were compared between the two groups in 2 years followed up. Results: The termination rate due to pregnancy, removal, or disease of women (1.9%) in group A was significant lower than that of women(9.5%) in group B (P=0.006), and the continuation rate of women (95.1%) in group A was significant higher than that of women (86.9%) in group B(P=0.008). The incidence of irregular vaginal bleeding and intrauterine adhesion of women (5.0% and 2.5%) in group A had no statistically significant different to those of women(6.3% and 2.4) in group B (P=0.679，0.410). The rate of menorrhagia and amenorrhea of women (6.3%)in group A was significant higher than that of women  (1.2%)in group B（P=0.019）, while the incidences of menstrual disorder, menstrual increase or lumbar abdominal pain of women(6.9%, 1.2% or 1.9%) in group A were significant lower than those of women(14.4%, 5.6%, or 8.1%) in group B (P=0.029, 0.032, 0.010). Conclusion: Subcutaneous implantation and IUD used immediately after abortion have both good contraceptive effect and higher continually used rate. The adverse reaction of subcutaneous implantation is lower, so it is worthy of clinical application.

2019 Vol. 27　(4):　461- [Abstract]（ 432 ） HTML (0 KB)  PDF  (0 KB)  ( 38 )

To explore the clinical efficacy of estradiol valerate combined with pelvic floor rehabilitation for treating patients with pelvic floor dysfunction. Methods: 92 patients with pelvic floor dysfunction were divided into study group and control group randomly. The patients in control group were treated by pelvic floor rehabilitation, and the patients in study group were treated by oral estradiol valerate (1mg/d) combined with pelvic floor rehabilitation. The left and right anal fissure diameter, anterior and posterior diameter and area (LHLP, LHAP and LHA), and the pelvic floor muscle strength of patients in the two groups were measured by the perineum threedimensional ultrasound and compared between before and after treatment. The pelvic floor dysfunction questionnaire (PFIQ-7) was used to assess the change of the pelvic floor function of patients. Sexual quality of life questionnaire (PISQ-12) was used to assess the sexual life quality of patients. Results: After treatment, LHLP, LHAP and LHA of patients in both groups at rest, anal contraction and Valsalva action were significantly decreased (P<0.05), and LHLP, LHAP and LHA of patients in study group were significant lower than those of patients in control group (P<0.05). The pelvic floor muscle strength of patients in both groups was improved significantly after treatment (P<0.05), and the pelvic floor muscle strength improvement of patients in study group was significant better than that of patients in control group (P<0.05). The PISQ-12 scores of patients in both groups increased significantly after treatment, and the PISQ-12 scores of patients in study group was significant higher than that of patients in control group (P<0.05). The PFIQ-7 scores of patients in both groups was decreased significantly, and the PFIQ-7 score was significant lower than that of patients in control group (P<0.05). Conclusion: Estradiol valerate can further improve the clinical efficacy of patients with pelvic floor dysfunction.

2019 Vol. 27　(4):　469- [Abstract]（ 399 ） HTML (0 KB)  PDF  (0 KB)  ( 41 )

To study the effect of methotrexate intramuscular injection combined with balloon compression for preventing heavy bleeding of pregnant women with placenta previa during cesarean section, and to explore its impact on pregnancy outcomes. Methods: 65 women with causative placenta previa who underwent caesarean section were selected as the study subjects from September 2016 to September 2017, and were divided into control group (n=32) and study group (n=33) according to the random number table method. The patients in the control group received carboprost trometamol injection, and the patients in the study group were given intramuscular methotrexate combined with balloon compression. The volume of blood loss during operation, menstruation recovery time, pregnancy outcomes, and complication rate were compared between the two groups. Results: The total volumes of blood loss and blood transfusion, time of bloody lochia, menstruation recovery time, time of postoperative hospital stay, and rate of adverse pregnancy outcomes (3.0% Vs. 15.7%) of women in study group were significant lower than those of women in control group (P<0.05). The incidence of postoperative complications (such as infection, vomiting, fever, and anemia) of women in study group was 9.1%, which had no significant different to that (21.9%) of women in control group (P> 0.05). Conclusion: Methotrexate intramuscular injection combined with balloon compression can effectively reduce the volumes of bleeding of women with dangerous placenta previa during cesarean section, and can reduce the rates of adverse pregnancy outcomes and complications.

2019 Vol. 27　(4):　476- [Abstract]（ 362 ） HTML (0 KB)  PDF  (0 KB)  ( 35 )

To investigate the influence of different regimens of dydrogesterone or progesterone for treating women with threatened abortion on pregnancy outcomes. Methods: 82 women with threatened abortion were selected as study subjects from February 2017 to February 2018, and were randomly divided into control group and observation group (41 cases in each group) according to random numbers table. The women in control group were treated by dydrogesterone, and the women in observation group were treated by progesterone. The clinical curative effect of women was compared between the two groups. The changes of levels of serum progesterone (P) and β-human chorionic gonadotropin (β-hCG), pregnancy outcomes, and drug safety of women in both groups were observed before and after treatment. Results: The overall curative effect of women had no significant different between the two groups (P>0.05). There was no significant different in levels of P and β-hCG between the two groups before and after treatment (P>0.05), but the levels of P and β-hCG of women in both groups were significantly increased after treatment (P<0.05). The rate of continually pregnancy of women in observation group had no significant different that of women in control group (P>0.05). The total incidence of adverse reactions of women in observation group was significant lower than that of women in control group (P<0.05). Conclusion: Dydrogesterone and progesterone are similar efficacy for treating women with threatened abortion, which can regulate hormone and improve the pregnancy outcomes. However, progesterone has more advantages in terms of drug safety, so it is worthy of clinical application.

2019 Vol. 27　(4):　484- [Abstract]（ 379 ） HTML (0 KB)  PDF  (0 KB)  ( 40 )

To investigate the TORCH infection of women before pregnancy, and to analyze the correlation of TORCH infection and the rate of stillbirth or spontaneous abortion of women after pregnancy. Methods: Peripheral blood samples from 1705 women who experienced the pre-pregnant examination were collected from January 2015 to January 2017. The infection situations of toxoplasma gondii (TOX), rubella (RV) and cytomegalovirus (CMV) were detected by enzyme-linked immunosorbent assay. The pregnant women were followed-up, and the relationship between the rate of TOX, RV, CMV infection of women and their pregnancy outcomes were analyzed. Results: A total of 119 (7.0%) women with TORCH infections in the included 1705 women were diagnosed, and among them, there were 24 women with TOX infection (1.4%), 42 women with RV infection (2.5%), 53 women with CMV infection (3.1%). The infection rates of TOX, RV and CMV of women with 20-30 years old were significant higher than those of women with 31-39 years old (P<0.05). 1620(95.0%) women were followed-up and 1102(68.0%) women were pregnant. In these 1102 pregnant women, there were 38 women (3.45%) with stillborn, 43 women (3.9%) with spontaneous abortion, and 81 women (7.35%) with abnormal pregnancy. The rate of adverse pregnancy outcomes of women with TORCH infection was significant higher than that of women without TORCH infection (P<0.05). The rates of stillborn and stillborn, and spontaneous abortion of women with TOX IgG(﹢),TOX IgM(﹢),RV IgG(﹢),CMV IgG(﹢), or IgM(﹢) were significant higher than those of women with TOX IgG(-),IgM (-),RV IgG(-), CMV IgG(-), or IgM (-)(P<0.05). Women with TOX IgG(﹢),TOX IgM(﹢),RV IgG(﹢),CMV IgG(﹢), or IgM(﹢) were the independent risk factors of stillbirth and spontaneous abortion occurred. Conclusions: TORCH infection is closely related to stillbirth, stillbirth and other adverse pregnancy outcomes, and women with 20-30 years old have higher rate of TORCH infection, so pre-pregnancy TORCH screening should be strengthen.

2019 Vol. 27　(4):　490- [Abstract]（ 362 ） HTML (0 KB)  PDF  (0 KB)  ( 31 )

To analyze the high risk factors of biochemical pregnancy of patients with recurrent spontaneous abortion (RSA). Methods: A total of 185 patients with RSA were divided into study group (95 patients with history biochemical pregnancy) and control group (90 patients without history biochemical pregnancy) between January 2016 and March 2018. The anatomical, endocrine, immune, or prethrombotic situation of women with RSA was analyzed, and multivariate logistic regression analysis was used to study the high risk factors of biochemical pregnancy of women. Results: Impaired fasting glucose, in vitro fertilization(IVF), equal to or over 3 times abortion, and abnormal autoimmune were risk factors for biochemical pregnancy (P<0.05). Multivariate logistic regression analysis also showed that abnormal autoimmune, IVF, or equal to or over 3 times abortion was independent risk factor for biochemical pregnancy. Conclusion: IVF is not an effective measure for treating patients with RSA. In order to better therapeutic effect and pregnancy outcomes, the causes of patients with RSA should be explore actively in clinical practice and should be treated based on these causes.

2019 Vol. 27　(4):　498- [Abstract]（ 333 ） HTML (0 KB)  PDF  (0 KB)  ( 40 )

To explore the influence of prenatal depression on pregnancy outcomes and to study its risk factors. Methods: The clinical data of 349 pregnant women between June 2015 and June 2017 were retrospectively analyzed. According to their prenatal depression, 140 women with prenatal depression were in group A, and 209women without prenatal depression was in group B. The general data and pregnancy outcomes of women were compared between the two groups, and logistic regression analysis was used to analyze risk factors for prenatal depression. Results: There were statistically significant differences in pregnancy stage, education level of pregnant women, family monthly income, relationship of husband and wife, relationship of women and their mother-in-law, situation of pregnancy preparation, number of prenatal examination, and prevalence of gestational diseased between the two groups (P<0.05). The logistic regression analysis showed that pregnancy stage, education level, occupation, relationship of husband and wife, number of prenatal examination, and prevalence of gestational disease were all independent risk factors for prenatal depression (P<0.05). Compared to those of women in group B, the cesarean section rate of women in group A was significant higher, time of the first stage of labor was significant longer, the rate of postpartum hemorrhage was significant higher, the rate of Apgar score and neonatal birth weight was significant lower (P<0.05). There was no significant difference in the rate of premature birth between the two groups (P>0.05). Conclusion: Prenatal depression is affected by social, physical and psychological factors, which can negatively impact on the pregnancy outcomes.

2019 Vol. 27　(4):　504- [Abstract]（ 382 ） HTML (0 KB)  PDF  (0 KB)  ( 40 )

To investigate the recurrence situation of pelvic inflammatory disease (PID) of outpatients, and to analyze the related factors influenced the recurrence of PID. Methods: The data of 900 outpatients with PID were collected from February 2013 to February 2017. The PID recurrence situations of these women within 2 years after treatment were recorded. The risk factors associated with recurrence of PID were analyzed by multiple factor logistic regression analysis. Results: The recurrence rate of PID of outpatients was 26.2% and the outpatients with recurrence more than once accounted for 34.3%. There were significant different in the rates of PID recurrence among outpatients with different course of disease, history of abortion or cesarean section, frequency of sexual life, number of sexual partners, and the status of cervicitis, contraception, sleep, and the diet (P<0.05).But There were no significant different in the rates of PID recurrence among outpatients with different ages, household registration, education, gravidity and parity, income level, and occupational and marital status (P>0.05). Multiple factor logistic regression analysis showed that the course of disease equal to or over 3 months before the first visited to doctor, history abortion or cesarean section, cervicitis, high sex life frequency, sexual partner equal to or over 2 persons, non condom used when sexual intercourse, sleeping time less than 8h every day, and irregular diet were the risk factors for PID recurrence of outpatients (P<0.05). Conclusion: Outpatient with PID who has long course of disease before the first visiting to doctor, history abortion or cesarean section, cervicitis, high sex life frequency, several sexual partner, non condom used when sexual intercourse, less sleeping time, and irregular diet should be strengthen the education, treatment, and following up for reducing the rate of PID recurrence.

2019 Vol. 27　(4):　511- [Abstract]（ 344 ） HTML (0 KB)  PDF  (0 KB)  ( 37 )

To investigate the disease-causing mutation in a Chinese family with congenital cataract. Methods: Direct sequencing on hotspot exons was applied to examine the DNA sample of the proband from a Chinese family with congenital cataract. Disease-causing mutation was identified by bioinformatics analysis and followed by ACMG classification. Results: A compound heterozygous mutation, c.569A>G (p.N190S) locus on GJA8 of the proband were found, which conformed to the law of genetic cosegregation in the family. Conclusion: c.569A>G (p.N190S) locus on in GJA8 maybe cause congenital cataract of patients in a Chinese family, and it is the first reported.

2019 Vol. 27　(4):　518- [Abstract]（ 239 ） HTML (0 KB)  PDF  (0 KB)  ( 39 )

Pernicious placenta previa (PPP) combined with placental implantation can lead to serious obstetric complications, and it is very important for prenatal diagnosis of PPP combined with placenta implantation. Ultrasound is currently recognized as the preferred method for diagnosing placental implantation. Some studies have shown that different ultrasound signs have different sensitivities and specificities, and comprehensive evaluation can improve the diagnostic rate of placenta implantation. Base on the position of the placenta , placental thickness, placental hypoechoic band, bladder wall continuity, placental lacuna, placental basal blood flow signal, cervical morphology and cervix sinus by ultrasound, and combined with previous cesarean section history of patients, etc, the scores can be received and can be used for evaluating the type of placental implantation and predict bleeding risk. The higher the scores are, the more severe the placental implantation is. In summary, prenatal ultrasound diagnosis of the type of placental implantation and prediction of bleeding risk has high accuracy and important clinical guiding value.

2019 Vol. 27　(4):　539- [Abstract]（ 375 ） HTML (0 KB)  PDF  (0 KB)  ( 33 )