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WANG Jingjing, YAO Yating, LI Kuo, ZHANG Yong, ZHANG Tian, WANG Minkang*
To explore the spermicidal and sperm agglutinated efficacy of red kidney bean lectin (RKBL). Methods: ① Sander Cramer test has been used to observe the spermicidal efficacy when mixed 5, 1, 0.5, 0.25, 2.5, 01, 0.05, 0.025, 0.01, 0.005, 0.0025, 0.001 mg/ml of RKBL gels with the same volume of semen in 20s, and the lowest dose of RKBL which killed all sperm in 20s was also explored, and the effective concentration of RKBL on inducing sperm agglutination was also observed. ②5, 1, 0.5, 0.25, 2.5, 0.1, 0.05, 0.025, 0.01, 0.005, 0.0025, 0.001 mg/ml of RKBL gels were put into the bottom of the “V” pipe, then added equal volume HTF medium to both side of the “V” type pipe. After that, Sperm was added into one side of the pipe, and then the effect of stopping sperm penetration and killed sperm were observed. Results: when the RKBL gel concentration was 1mg/ml or above, sperm was inactivation and instant death, so the lowest dose for effective killing sperm by the Sander Cramer test in 20s was 1mg/ml. Sperm agglutination had occurred when RKBL gel concentration was 0.01mg/ml or above. In “V” type pipe, different concentrations of RKBL gel could prevent sperm penetration. When the concentration of RKBL gel was 1mg/ml or above, the sperm completely lost activity near the gel. Conclusion: The lowest effective concentration of RKBL gel for killing sperm is 1mg/ml or above. Some low concentration of RBKL gel can induce sperm aggregate in different degrees. The different
DOI:10.3969/j.issn.10048189.2017.10.002
2017 Vol. 25 (10): 654- [Abstract]（
500
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FANG Liwei, LI Zuoshan*, XIANG Qiqi, CHEN Xiaoke
To investigate the reproductive motivation of the post80’s generational parents after national universal twochild policy and to explore its influencing factors. Methods: The multistage sampling method was used to select 626 post80’s generational parents from Chongqing, and the self designed questionnaire about post80’s generation of parents reproductive motivation and its influencing factors was used as a tool for investigation. Results: ① There were significant different in the scores of reproductive motivation in the demographic characteristics as whether or not the only child, the gender of the first child, educational level and family annual income between the parents from urban and rural (P<0.001); ②The scores of caring on the family line, raising children for supporting parents in their old age, promoting family harmony, benefit for child growth and education, improving family status, and personal ideal sustenance of parents wanted to reproducing group were significantly higher than those of parents unwanted to reproducing group again (P<0.001); ③Gradual multiple regression analysis showed that childhood loneliness, the pressure of supporting the elderly, degree of elders urged and the optimistic of educational resources positively affected the scores of reproductive motivation (β=0.313, 0.261, 0.158, 0.093, P<0.001), but the dispersion degree of parental energy when childhood negatively affected the scores of reproductive motivation (β=0.250, P<0.001). Conclusion: Caring on the family line, raising children to support parents in their old age, promoting family harmony, benefit for child growth and education, improving family status, and personal ideal sustenance have plaid the dominant role in the reproductive decisionmaking of post80’s generational parents. And reproductive motivation are mainly affected by childhood loneliness, the pressure of supporting the elderly, the dispersion degree of parental energy when childhood, degree of elders urged, and the optimistic of educational resources and so on.
2017 Vol. 25 (10): 658- [Abstract]（
667
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JI Guoping
To explore the linear relation between the number of women taking folic acid and the rate of neonatal neural tube defects (NTD) in Anhui province, and to provide evidence for evaluation of the effect of the project of taking folic acid to prevent neural tube defects (folic acid project). Methods: The data of women who took folic acid and data of women who had good compliance of taking folic acid were selected from the report of folic acid project since 2009 to 2015. And the NTD rate was selected from the birth defect surveillance system since 2009 to 2015. The linear relations analysis between the number of women who took folic acid and NTD rate was analyzed, and the linear relations between the number of women who had good compliance of taking folic acid and NTD rate was also analyzed. Results: From 2009 to 2015, the number of women who took folic acid and the number of women who had good compliance of taking folic acid were both increasing year by year. The number of women who took folic acid and the number of women who had good compliance of taking folic acid in rural were higher than those in urban. The total NTD rate was decreasing (χ2=15.33, P<0.001), that in urban was fluctuating (χ2=0.17, P=0.677) and that in rural NTD was decreasing (χ2=26.06, P<0.001). The gap of NTD rate between urban and rural had decreased from 5.84/10000 in 2009 to 0.64/10000 in 2015. In rural, the number of women who had good compliance of taking folic acid and NTD rate had preferable linear relations (Y=13.12 2.36X, adjR2=0.94, P<0.001). Conclusion: In Anhui province, there was a linear relation between the number of women who has good compliance of taking folic acid and NTD rate by the birth defect surveillance system reported. The folic acid project plays an important role for reducing NTD rate in rural areas, and the gap between urban and rural areas has reduced. Increasing compliance of women taking folic acid is the key for implementation the folic acid project successfully.
2017 Vol. 25 (10): 662- [Abstract]（
393
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FENG Nana1,2,SHI Baoxin1
To explore pressure current situation of man with infertility, and to study the association between man with infertility pressure and their role conflict. Methods: A crosssectional survey was implemented, and the questionnaire was selfdesigned by researchers, which included demographic information sheet, fertility problem inventory (FPI) and gender role conflict survey (GRCS). 156 male patients with infertility (study group) and 190 men whose partners with infertility (comparison group) filled in the questionnaires. Results: The total scores of FPI（162.7±27.1）, the scores of social concern , relationship concern, and sexual concern of the 156 male patients were significant higher than those of men in comparison group (P<0.05). Multiple regression analysis results showed that poor expressing their emotional feelings was an important factor for influencing infertility stress. Conclusion: Male patients with infertility are more likely to have social and sexual pressure, and have more strained relationship with their partner. Male patients are also more stressed if their emotional feelings expressing is poor. So the special socialpsychology of male patients with infertility should be paid more attention in practical work.
2017 Vol. 25 (10): 666- [Abstract]（
454
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LUO Mingzhong1， ZHANG Zhen2，ZHANG Chunmei1，LEI Lijian2*
To investigate the reproductive health knowledge and status among people with reproductive age from the pilot areas of reproductive health promotion project in Shanxi Province for improving the pertinence and effectiveness of the project. Methods：From Apr. 2016 to Aug. 2016, convenient sampling method was used to select the subjects from the grassroots level maternal and child health care and family planning service stations in the pilot areas of reproductive health promotion project in Shanxi Province. The questionnaires investigation was conducted in subjects with 1565 years old. Results：964 people were investigated. The rate of correct answering reproductive health related knowledge had significantly different（P<005） in people with different educational backgrounds , age, household registration and history of abortion. The rate of correct answered about knowledge of women and children health was 863%, and the rate of correct answered about knowledge of sexually transmitted/HIV/AIDS were 56.0%. The prevalence of reproductive system diseases of people with different sex, age, educational backgrounds and history of abortion were statistical significant different (P<0.05). Multivariate logistic regression analysis showed that the disease of reproductive system was related to the induced abortion history (P <0.001), and was also associated with age both in male and female (P=0.023, P=0.031). Conclusion：Reproductive health knowledge is poor in populations with lower age and lower education level. And knowledge of sexually transmitted HIV/AIDS is deficiency for the investigated people. Adolescents and women with abortion history have higher risk of reproductive systemrelated disease. Primary health care institutions should carry out the corresponding reproductive health services, increase the reproductive health education coverage and improve the reproductive health status of the people.
2017 Vol. 25 (10): 671- [Abstract]（
631
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NI Wenli1, WANG Baojun2, TIAN Tian1, ZHANG Ji1, MA Le2, WANG Linlin1*, REN Aiguo1
To investigate the relationship between macromolecular oxidative damage in seminal plasma and semen parameters. Methods: A total of 96 men who came to seek infertility treatment were recruited as subjects. A facetoface questionnaire was administered by the trained investigators. Semen samples were collected by masturbation. Routine semen parameters were determined by computeraided sperm analysis system. Protein oxidative damage and lipid oxidative damage were showed by the concentration of protein carbonyl derivatives (PC) and 8isoprostaglandin F2α (8isoPGF2α), respectively, and the seminal plasma was measured by enzymelinked immunosorbent assay. To examine the association of semen parameters with oxidative damage indexes, all subjects were divided into normal group and abnormal group according to the normal range of sperm concentration, progressive motility rate and teratozoo spermia rate. Results: 8isoPGF2αconcentration of subjects in abnormal sperm concentration group was higher than that of subjects in normal sperm concentration group (P<0.05). There was no significant difference in PC concentration between normal sperm concentration group and abnormal sperm concentration group. No significant differences were found in PCand 8isoPGF2αconcentration between normal and abnormal groups in term of progressive motility rate and teratozoospermia rate. Pearson correlation analysis showed that 8isoPGF2α concentration was negatively correlated with sperm concentration (r=0.254, P=0.018), but uncorrelated to other sperm parameters; PC concentration was not significantly correlated with any semen parameters. Multiplelinear regression analysis showed that sperm concentration decreased 7×104/ml by1 ng /ml increase of 8isoPGF2α. Conclusion: The decrease of sperm concentration may be caused by lipid oxidative damage in men.
2017 Vol. 25 (10): 676- [Abstract]（
495
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YAO Yongchang
To explore the effect of early using hemabate for preventing and treating postpartum hemorrhage caused by uterine inertia after cesarean section. Methods：From March 2012 to July 2017, 90 women experienced cesarean section with bleeding risk factors in Zhongda hospital were enrolled and randomly divided into three groups (30 cases in each group). All women in the three groups had received injection of oxytocin immediately after fetal delivery, women in the instant group had received hemabate 250μg immediately after fetal delivery, women in the selection group had received hemabate 250μg when uterine inertia, and women in the control group had not given hemahate. Among the three groups, the volume of bleeding, value of systolic blood pressure (SBP) , diastolic blood pressure (DBP) of women intraoperative, 2 hours and 24h hours postpartum were compared, the rate of postpartum hemorrhage was compared, and the amplitudes of hemoglobin (Hb) decreased of women 24h hours postpartum was also compared. Results: The volume of bleeding intraoperative, 2 hours and 24h hours postpartum, and the rate of Hb decreased 24h hours postpartum of women in the instant group were significantly lower than those of women in the another two groups. The rate of postpartum hemorrhage of women in the instant group was 20.0%, which was significantly lower than that of women (46.7%) in the selection group (P<0.05), and the rate of postpartum hemorrhage of women in the selection group was also significantly lower than that of women (60.0%) in the control group (P<005). The value of SBP and DBP of women had no significant differences among the three group (P<0.05). The adverse reactions rate of women in the control group was 3.3%, which was significantly lower than that of women in the instant group (23.3%) and the selection group (20.0%)(P<0.05). Conclusion: The early application of hemabate after fetal delivery by cesarean section can significantly reduce the valume and rate of postpartum hemorrhage caused by uterine inertia, which can effectively prevent and treatment postpartum hemorrhage.
2017 Vol. 25 (10): 680- [Abstract]（
474
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YIN Guanmei, HAN Yaowei, ZHANG Chunhua, LIU Jia
To explore the clinical curative effects of three kinds of pelvic floor muscle treatments on functional recovery of pelvic floor muscle of puerperae with stress urinary incontinence, and to explore the better nonsurgical methods to treat puerperae with stress urinary incontinence. Methods: From September 2014 and January 2016, a total of 107 puerperae with stress urinary incontinence who accepted postpartum 42 day examination were selected and randomly divided into 3 groups. 30 puerperae who had received single electrical stimulation was in group A, 31 puerperae who had received single scene biofeedback training was in group B, 46 patients puerperae who had received electrical stimulation combined with scene biofeedback training was in group C. The determination of values of all puerperae before treatment were the primary screening results, the determination of values of all puerperae after treatment for 5 times were secondary screening results, and the determination of values of all puerperae after treatment for 10 times were the third screening results. The change of screening results of puerperae after three different treatments were evaluated on the muscles strength, fatigue and muscle potential of the pelvic bottom. Results: The results showed that compared to the primary screening results, there were statistically significant differences in the level I muscle strength and level II muscle strength of secondary screening results and third screening results of puerperae (P<0.05), but there were no statistically significant differences in degree of I muscle fatigue, level II muscle fatigue, and muscle potential of puerperae in the three groups(P>0.05). After treatment, the level I or level II muscle strength, and the degree of I muscle fatigue or II muscle fatigue the primary screening results of all women had no statistically significant difference (P>0.05). Muscle potential of women in group B and group C was higher than that of women in group A(P<0.05). Conclusion: The three kinds of treatment all can improve the muscle strength of puerperae with stress urinary incontinence. The average self score of women in the three groups is more than 7 points, and the degree of urine leakage of women has obviously improved comparing to before treatment. It is suggest that at least 5 times pelvic floor rehabilitation therapy can improve pelvic floor muscle strength recovery, and electrical stimulation combined with scene biofeedback training or scene biofeedback training has the best curative effect for muscle potential.
2017 Vol. 25 (10): 685- [Abstract]（
426
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ZHENG Weiwei, SONG Ge, WU Zheng, LIU Shanwen, ZHU Xiaoli, TAN Yumei
To explore the optimal sperm concentration for insemination when patients undergone vitro fertilizationembryo transfer(IVFET）used donor sperm by compared the influence of different sperm concentration for insemination on rate of fertilization, and to explore the influence of fertilization methods on clinical outcomes by compared with intracytoplasmic sperm injection (ICSI) used donor spermatozoa. Methods: The date of 542 cycles of routine IVF used donor spermatozoa from January 2013 to June 2016 was retrospectively analyzed. The subjects were in three groups according to the different sperm concentrations for insemination, which included that subjects in group A used sperm concentration were（1.02.0）×106/mL，patients in group B used sperm concentration were（2.04.0）×106/mL，and patients in group C used sperm concentration were（4.08.0）×106/mL. Fertilization rate，polyspermy fertilization rate，cleavage rate,quality embryo rate，implantation rate, pregnancy rate and abortion rate were compared among the three groups. The distinction of clinical outcomes were also compared between the patients experienced IVFET and the subjects experienced ICSI). Results: ① There was no significant difference in fertilization rate，cleavage rate, quality embryo rate , implantation rate, pregnancy rate and abortion rate among the three groups（P > 0.05）. However，the polyspermy fertilization rate of patients in group B and group C were significantly higher than those of patients in group A（5.66%，5.79% VS 2.69%，P ＜ 0.05）.②when the number of donor spermatozoa was enough for routine IVF, ICSI could not improve quality embryo rate and pregnancy rate. Conclusion: Optimizated sperm insemination concentration for IVFET when donor sperm concentration were（1.02.0）×106/mL, fertilization rate, implantation rate and pregnancy rate are high. Increasing the sperm concentration of insemination cannot improve the clinical outcomes, but maybe increase the risk of polyspermy fertilization. ICSI can not increase the quality of embryo and pregnancy rate when donor spermatozoa is enough for IVF.
2017 Vol. 25 (10): 690- [Abstract]（
403
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WANG Yue, ZHAN Rui, WANG Yeqing, YANG Mei
To explore the clinical effect of epidural combined intravenous anesthesia in laparoscopic hysterectomy. Methods: The data of 80 patients who experienced laparoscopic hysterectomy in Xuancheng people's hospital from January 2014 to December 2016 were collected. According to the different anesthesia methods, patients were divided into the observation group and the control group, 40 cases in the observation group were given epidural anesthesia combined with general anesthesia, and 40 cases in the control group were given intravenous anesthesia. The anesthesia effect, postoperative extubation time, spontaneous breathing recovery time, awake time, rate of postoperative agitation, nausea and vomiting, operation time, total hospitalization expenses and the rate of postoperative additional analgesic drugs of patients in the two groups were compared. Results: Compared to the control group, the total effective rate of patients in the observation group was significantly higher (97.5% vs. 80%), and the difference had statistically significant (P<0.05). Furthermore, the tracheal extubation time, spontaneous breathing time, and awake time of patients in the observation group were significant shorter than those of patients in the control group (P<0.05), and the incidence of postoperative agitation and the additional analgesic drugs rate of postoperative additional analgesic drugs analgesia were significantly lower than those of patients in the control group (P<0.05). However, the operation time, hospitalization expenses and incidence of adverse reactions of patients had no significantly different between the two groups (P>0.05). Conclusion: The effect of epidural combined intravenous general anesthesia used for laparoscopic hysterectomy is better than intravenous anesthesia alone, and it does not increase the rate of adverse effects of surgery and the total cost of hospitalization, so it is worthy of clinical application.
2017 Vol. 25 (10): 694- [Abstract]（
469
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GAO Jing, ZHANG Ying, CHEN Lin
To investigate the application values of colposcopic fourquadrant biopsy for diagnosing cervical cancer. Methods: A retrospective study was conducted to analyze the clinical data of 150 patients with positive result by cervical thinprep cytologic test (TCT) from January 2015 to June 2016 in Affiliated Hospital of Nankai University. All patients were further checked by colposcopy checked, cervical fourquadrant biopsy under colposcopy or suspicious lesions biopsy under colposcopy, and tissue from biopsy was further pathological determined. The patients with pathological positive result of biopsy tissue had been operated for removing lesions tissue. And postoperative pathological result was the final diagnosis. Results: 48 cases (32.0%) had negative results and 102 cases (68.0%) had positive results by colposcopy checked. The negative rate of postoperative pathology was 36.7% (55 cases), and 95 cases (63.3%) were positive pathological results. The coincidence rate diagnosed by colposcopic biopsy and postoperative pathology was 87.3%. Among them, the coincidence rate of diagnosed LSIL, HSIL and invasive carcinoma by fourquadrant biopsy under colposcopy and by postoperative pathology were 71.4%, 81.6% and 100%, respectively. The coincidence rate of diagnosed LSIL, HSIL and invasive carcinoma by suspicious lesions biopsy under colposcopy and postoperative pathology were 68.2%, 73.7%, 88.2%, respectively. The rate of diagnostic accuracy of cervical cancer was higher than that of LSIL and HSIL (P<0.05). The coincidence rate of diagnosis LSIL and above LSIL by four quadrant biopsy under colposcopy was higher than that by suspicious lesions biopsy under colposcopy (P<0.05). The sensitivity, accuracy and positive predictive value (91.7%, 98.0%, 100.0%) of fourquadrant biopsy under colposcopy for diagnosing cervical cancer were significantly higher than those of suspicious lesions biopsy under colposcopy (P<0.05). Conclusion: Fourquadrant biopsy under colposcopy has great value in the early screening of cervical cancer, which can improve the detection rate and diagnostic accuracy of cervical cancer.
2017 Vol. 25 (10): 697- [Abstract]（
498
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