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| Influence of different sperm concentration for insemination and fertilization methods on clinical outcomes of patients when undergone IVF used donor spermatozoa |
| The Reproductive Centre of the Family Planning Special Hospital of Guangdong Province , Guangzhou, Guangdong, 510060 |
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Abstract To explore the optimal sperm concentration for insemination when patients undergone vitro fertilizationembryo transfer(IVFET）used donor sperm by compared the influence of different sperm concentration for insemination on rate of fertilization, and to explore the influence of fertilization methods on clinical outcomes by compared with intracytoplasmic sperm injection (ICSI) used donor spermatozoa. Methods: The date of 542 cycles of routine IVF used donor spermatozoa from January 2013 to June 2016 was retrospectively analyzed. The subjects were in three groups according to the different sperm concentrations for insemination, which included that subjects in group A used sperm concentration were（1.02.0）×106/mL，patients in group B used sperm concentration were（2.04.0）×106/mL，and patients in group C used sperm concentration were（4.08.0）×106/mL. Fertilization rate，polyspermy fertilization rate，cleavage rate,quality embryo rate，implantation rate, pregnancy rate and abortion rate were compared among the three groups. The distinction of clinical outcomes were also compared between the patients experienced IVFET and the subjects experienced ICSI). Results: ① There was no significant difference in fertilization rate，cleavage rate, quality embryo rate , implantation rate, pregnancy rate and abortion rate among the three groups（P > 0.05）. However，the polyspermy fertilization rate of patients in group B and group C were significantly higher than those of patients in group A（5.66%，5.79% VS 2.69%，P ＜ 0.05）.②when the number of donor spermatozoa was enough for routine IVF, ICSI could not improve quality embryo rate and pregnancy rate. Conclusion: Optimizated sperm insemination concentration for IVFET when donor sperm concentration were（1.02.0）×106/mL, fertilization rate, implantation rate and pregnancy rate are high. Increasing the sperm concentration of insemination cannot improve the clinical outcomes, but maybe increase the risk of polyspermy fertilization. ICSI can not increase the quality of embryo and pregnancy rate when donor spermatozoa is enough for IVF.
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