To investigate the changes of the levels of serum carbohydrate antigen 125 (CA125) and matrix metalloproteinase (MMP-3) of patients with adenomyosis  (AM) after the high intensity focused ultrasound (HIFU) combined with gonadotropin releasing hormone agonist (GnRHa) treatment, and to study their correlation with the dysmenorrhea severity of the patients. Methods: A prospective study was conducted to select 100 patients with AM who were treated with HIFU combined with GnRHa in the hospital from January 2022 to January 2024 as the research objects. The serum CA125 and MMP-3 levels of the patients were measured before treatment, and in the 3rd month and in the 6th month after treatment. The severity of dysmenorrhea of the patients was evaluated by visual analogue scale (VAS) score. The correlation between the changes of the serum CA125 and MMP-3 levels of the patients and the severity of their dysmenorrhea was analyzed by bivariate Pearson (N). According to the severity of dysmenorrhea of 
the patients before treatment, these patients were divided into group A (48 cases with mild dysmenorrhea), group B (33 cases with moderate dysmenorrhea) and group C (19 cases with severe dysmenorrhea). The clinical baseline data and the serum CA125 and MMP-3 levels of the patients were compared among the three groups. The influencing factors of the dysmenorrhea severity of the patients were analyzed by ordinal logistic regression. Results: The levels of serum CA125 and MMP-3 and the VAS score of the patients before treatment (72.99±6.44 U/ml, 43.60±5.76 ng/ml and 7.10±1.26 points), in the 3rd month after treatment (65.57±5.64U/ml, 19.47±5.86ng/ml and 2.27and 2.27±0.59 points), and in the 6th month after treatment (43.51±4.58U/ml, 14.06±4.38ng/ml and 1.31±0.43 points) had decreased gradually. Bivariate Pearson (N) analysis showed that the serum CA125 and MMP-3 levels of the patients were positively correlated with their VAS score of dysmenorrhea. The lesion volume, the gravidity and the serum CA125 and MMP-3 levels of the patients in group A, in group B and in group C had increased gradually. Logistic regression analysis showed that the increased lesion volume, the gravidity and the serum CA125 and MMP-3 levels of the patients with AM were the independent factors affecting their degree of dysmenorrhea (all P<0.05). Conclusion: HIFU combined with GnRHa for treating the patients with AM can increase their serum CA125 and MMP-3 levels and relieve their dysmenorrhea. At the same time, the serum CA125 and MMP-3 levels of the patients are positively correlated with their pain degree. The increased lesion volume, the gravidity and the serum CA125 and MMP-3 levels of the patients with AM are the independent factors affecting their degree of dysmenorrhea.

To discover the research progress of the female secondary infertility, and to explore the important research hotspots and the future development trends on the female secondary infertility. Methods: Taking the secondary infertility as the research theme, the literatures published in CNKI, Wanfang Database, SinoMed, Web of Science Core Collection and PubMed from the establishment of the database to December 31, 2023 were retrieved. The published languages of the literatures included in this study were limited in Chinese and English. The publication year, authors and cooperation network, keywords, etc. of the literatures were visualized and analyzed using VOSviewer software. Results: A total of 2330 domestic studies (2269 literatures published in Chinese and 61 literatures published in English) and 788 foreign studies were included. The number of the domestic studies related to the female secondary infertility began to increase in 1990 significantly, and the number of the literatures reached its peak in 2011. Since 2005, the number of the foreign research literatures related to the female secondary infertility increased significantly. A number of the stable research teams on the female secondary infertility had been formed. The top 10 keyword categories in the domestic research literatures related to the female secondary infertility were “secondary infertility” “clinical diagnosis and treatment” “associated factor” “investigation and analysis” “pregnancy” “fallopian tube” “ART” “endometrium” “nulliparous women” and “fertility”, and in which, the corresponding English keywords were also included. The top 10 keyword categories in the foreign research literatures related to the female secondary infertility were “secondary infertility” “associated factors” “diagnosis” “ART” “reproduction” “diseases” “treatment” “biology” “physiology” and “research methodology”. In the domestic research literatures related to the female secondary infertility, the overall changes in the frequency of the keywords had increased gradually except to "nullipara ". In the foreign research literatures related to the female secondary infertility, the keywords, such as "associated factors", "ART", "secondary infertility" and "diagnosis", had increased gradually with time.  Conclusion: The female secondary infertility has received a high level of attention overall, but the communication and cooperation among the different research teams need to be further strengthened. Continuing to explore the risk factors of the female secondary infertility plays an important role in the development of the comprehensive prevention and control strategies for the female secondary infertility. Therefore, the researches related to the female secondary infertility should be strengthened to provide more scientific evidences for further improving the female reproductive health. 

To understand the anxiety of unmarried women before induced abortion, to explore the influencing factors and its predictive value of the women, and to provide reference for the effective psychological intervention. Methods: A total of 148 unmarried women who planned to undergo induced abortion in the hospital from May 2020 to May 2022 were selected in this study. The self-made general information questionnaire, the self-rating anxiety scale (SAS) and the pain disclosure index scale (DDI) were used for the anonymous investigation. Univariate analysis and multivariate logistic regression analysis were used to explore the influencing factors of the anxiety of the unmarried women before induced abortion. Receiver operating characteristic (ROC) curve was used to analyze the predictive value of the related influencing factors of the unmarried women for their anxiety before induced abortion. Results: A total of 142 unmarried women who planned to undergo induced abortion were included in this study, and with the SAS score of 52.97±4.03 points. And among them, there were 102(71.8%) cases with anxiety, and the SAS score of the 102 women was 57.82±3.26 points. There were 40(28.2%) cases without anxiety, and the SAS score of these 40 women was 40.61±4.59 points. Univariate analysis showed that the age, the occupation, the history of induced abortion, the understanding of reproductive health knowledge, the abortion method, the contraceptive attitude of the sexual partners and the self-disclosure of the unmarried women were significantly related to their anxiety before induced abortion (P<0.05). Multi-factor analysis showed that the proposed medical abortion, the young women, the poor reproductive health knowledge level and the low level of self-disclosure of the unmarried women had significantly greater risk of their anxiety before induced abortion (P<0.05). ROC curve analysis showed that the each influencing factor of the unmarried women all had the good predictive value for their anxiety occurrence before abortion, and with the area under the curve of 0.622-0.655. Conclusion: The medical abortion, the young women, the poor reproductive health knowledge level and the low level of self-disclosure of the unmarried women are significantly related to their anxiety before induced abortion, and all of which has certain predictive efficacy for their anxiety before induced abortion, and so the clinical countermeasures should be taken accordingly.

To explore the microRNAs (miRNAs) and genes associated with asthenospermia based on bioinformatics methods, and to provide the new ideas for the diagnosis and treatment of asthenospermia. Methods: The miRNAs associated with asthenospermia were identified by screening the literatures in pubmed and Web of science, and then their target genes were predicted by TargetScan database. The data set GSE92578 related to asthenospermia selected from gene expression omnibus (GEO) and GEO2R software was used to analyze for obtaining Differential Expression Genes (DEGs). The target genes of the miRNAs and the DEGs from this analysis were intersected to obtain the final DEGs. The analysis of the gene ontology (GO) and the Kyoto encyclopedia and genomes (KEGG) genes signaling pathway enrichment of the DEGs were performed using the DAVID database. Then, the key genes were obtained by the analysis of the protein interaction (PI) network in Gene STRING database, and the node genes were obtained by cytoscape software and its plug in cytoHubba. Results: A total of 7 differentially expressed miRNAs and 84 differentially expressed genes were screened through the literature. The results of GO enrichment analysis showed that the biological processes (BP) involved by the differential genes were mainly included the homologous protein binding, the protein kinase binding and the protein serine/threonine kinase activities. The cellular components (CC) mainly included cytoplasm, cell membrane, lysosomal membrane, and acrosome, etc. The molecular functions (MF) mainly included the positive regulation of transcription from RNA polymerase II promoter, the positive regulation of protein translocation, extracellular matrix organization, and phosphorylation, etc. KEGG related pathways involved PI3K Akt signaling pathway, human papillomavirus infection pathway, platelet activation and chemoattenuation receptor activation pathways. The 10 node genes obtained by cytoscape were AKT1, MAPK3, BRD4, DNMT3A, FURIN, LMNB2, COL5A2, COL5A3, COL11A1 and COL27A1. Conclusion: The miRNAs, hub genes and the related pathways obtained in this study may play the important roles in the pathological process of the spermatogenesis of the patients, and which may provide the reference targets for the subsequent mechanistic studies on the spermatogenesis.

To understand the contraceptive status of women with induced abortion aged 25 years and below. Methods: In 15 hospitals from 10 provinces, a questionnaire survey was conducted on the contraceptive status of the pregnant women ≤25 years old who wanted induced abortion during the first trimester of pregnancy from March 2021 to August 2022. Results: 11.8% of the respondents were under 19 years old, 75.7% of the respondents were unmarried, 15.7% of the respondents had given birth and 31.5% of the respondents had induced abortion history. The reason for this unintended pregnancy, about half of them did not use any contraception. 1375 women with contraceptive failures, up to 47.1% of them used condoms. The correct rate of 8 questions about the effect of contraceptive method and its influence on the future fertility was less than 50%. More than 60% respondents were "not sure" about their future fertility planning and 542 (19.4%) respondents said they would choose long-acting reversible (LARC) after abortion or in the future. Logistic regression analysis showed that the age 24-25 years old, the history of induced abortion, the knowledge about that intrauterine device (IUD) as a highly effective contraceptive method, the knowledge about that the pregnancy preparing immediately after IUD or subdermal implant removal, and the desire of giving birth after 2 years or the desire without child in the future of the young women were the promoting factors for their choosing LARC. Conclusion: The contraceptive status of the women with induced abortion aged 25 years and below is worrying. It is necessary to strengthen the health education and consultation services to improve the implementation rate of LARC of the women with induced abortion aged 25 years and below, to effectively avoid induced abortion and to protect the fertility of the adolescent women. 

To investigate the status quo and the influencing factors of the disease acceptance of patients with cervical cancer. Methods: 160 patients with cervical cancer during in-patient and out-atient from January 2021 to December 2023 were selected as research objects. The score of illness ccceptance questionnaire (IAQ) of the patients was measured. The demographic data and disease characteristics of the patients were counted. Single factor and multiple factor analysis were used to screen the influencing factors of the disease acceptance of the patients with cervical cancer. Results: Among 160 patients with cervical cancer, there were 58 cases with low acceptance of the disease, accounting for 36.3%, there were 87 cases with moderate acceptance of the disease, accounting for 54.4%, and there were 15 cases with high acceptance of the disease, accounting for 9.4%. There were statistically significant differences in the IAQ score among the patients with different age, among the patients with different marital status, among the patients with different medical payment method, among the patients with different number of children, or among the patients with different stages, recurrence or metastasis of cervical cancer (P<0.05). The results of multiple regression model indicated that the young age, the spinsterhood, the medical payment method as the basic medical insurance of urban and rural residents, the less number of children, the high stage of cervical cancer, and the recurrence or metastasis of cervical cancer of the patients were all the influencing factors of their disease acceptance (all P<0.05). Conclusion: The affecting factors of the disease acceptance of the patients with cervical cancer should be focused on in clinic. The patients with cervical cancer should be given counseling and psychological support actively to increase the treatment compliance, to improve the treatment effect and to promote the postoperative recovery of the patients.

To investigate the sexual life quality of women after myomectomy, and to analyze its influencing factors and countermeasures. Methods: 98 patients with uterine fibroids who had undergone myomectomy in hospital from December 2020 to December 2023 were selected in this survey. The quality of sexual life of the patients was investigated by female sexual function index (FSFI). The psychological status and the marital adjustment level of the patients in 3 months after operation were evaluated by Hamilton anxiety scale (HAMA), Hamilton depression scale (HAMD) and marriage adjustment test (MAT). Multivariate logistic regression was used to analyze the influencing factors of the postoperative sexual life quality of the patients who had undergone myomectomy. Results: The postoperative score of the total FSFI each dimension (27.34±4.32 points) of 98 patients after operation was significantly lower than that (29.10±3.12 points) before operation (P<0.05). The age, the education level, the family monthly income, the occupational status, the surgical method, the psychological status and the marital adjustment level of the patients were all related to their postoperative sexual life quality (P<0.05). Multivariate logistic regression analysis showed that the advanced age (OR=3.155, 95%CI 1.309-7.607), the high HAMD score (OR=4.604, 95%CI 1.483-14.295) and the surgical method of laparoscopic myomectomy (OR=3.397, 95%CI 1.207-9.563) of the patients were all the independent factors affecting their postoperative sexual life quality. Conclusion: The postoperative sexual life quality of the patients with myomectomy is generally low. The advanced age, the surgical method of laparoscopic myomectomy and the low psychologic status of the patients are all the independent factors affecting their postoperative sexual life quality. Clinical intervention can be carried out by strengthening the propaganda and education, by providing the psychological nursing, by improving social support and by guiding the exercise.

To investigate the fear of childbirth and its influencing factors analysis of pregnant women during the third trimester of pregnancy. Methods: A convenience sampling method was used to select 490 pregnant women during the third trimester of pregnancy from September to December 2023 as the study subjects. The childbirth fear scale (CAQ), childbirth self-efficacy inventory (CBSEI-C32), and anxiety self assessment scale (SAS) were used to collect the situations of the fear of childbirth, the prenatal anxiety and the self-efficacy in childbirth of the women. The factors affecting the fear of childbirth of the women were analyzed. Results：The incidence of the childbirth fear of the women was 59.8%. Logistic regression analysis showed that the prenatal anxiety was an independent risk factor of the childbirth fear of the pregnant women during the third trimester of pregnancy (OR=1.861, 95%CI 1.224-2.829). The history of pregnancy and childbirth (OR=0.590, 95%CI 0.393-0.886), and the high score of childbirth in self-efficacy (OR=0.177, 95%CI 0.118-0.266) of the women were the independent protective factors of their childbirth fear during the third trimester of pregnancy. Conclusion: The rate of the childbirth fear of the pregnant women during the third trimester of pregnancy is higher, and the childbirth fear of the women is influenced by the factors, such as the pregnancy and childbirth history, the anxiety, and the self-efficacy in childbirth, which should be paid attention in the clinical work, and the targeted intervention strategies should be developed.

To explore the influence of the different intervention timing of the epidural analgesia for primiparas on their stages of labor and delivery outcomes. Methods: The data of 420 primiparas that had undergone vaginal trial delivery from January to December 2023 were selected in this study. Among them, 240 cases were treated with labor analgesia and were divided into group A (120 cases with labor analgesia at their latent phase of the uterus orifice dilation <4cm) and active analgesia group (120 cases with labor analgesia at their active phase of the uterus orifice dilatation ≥4cm) according to the timing of labor analgesia used. The other 180 primiparas who had not received labor analgesia were included in group C. The duration of labor, the delivery outcomes, the soft birth canal injuries, and the neonatal Apgar scores of the primiparas were compared among three groups. Results: The duration of the first, the second labor and the total labor time of the primiparas in group A were significantly longer than those of the primiparas in group C (q=4.71, 6.57, 6.02, P<0.05). The duration of the first and the total labor of the primiparas in group B were significantly shorter than those of the primiparas in group A (q=5.06, 4.23, P<0.05). The rates of cervical laceration and episiotomy of the primiparas in group A and in goup B were significantly lower than those of the primiparas in group C (χ2=5.91, 4.54, 7.30, 3.86, P<0.05). The incidences of the fetal distress and the cesarean section of the primiparas in group B were significantly lower than those of the primiparas in group C (χ2=4.71, 4.55, P<0.05). The incidence of the fetal abnormal position of the primiparas in group B was significantly higher than that of the primiparas in group C (χ2=3.87, P<0.05). There were no significant differences in the rates of the Ⅱ degree perineal laceration, the bleeding volume in 24 hours after delivery, the vaginal midwifery rate, and the neonatal Apgar scores of the primiparas among the three groups (all P>0.05). Conclusion: Although the epidural analgesia for the primiparas may cause their prolonged labor, it does not increase the risk of the adverse neonatal outcomes and the postpartum hemorrhage, and which helps to reduce the rate of cervical laceration and lateral episiotomy. However, the incidences of the duration of the total labor process, the abnormal fetal position, the fetal distress and the transfer to cesarean section of the primiparas with the labor active period analgesia are lower than those of the primiparas with the labor latent period analgesia, so 4cm of the uterus orifice dilation of the primiparas is considered as a more appropriate time for the labor analgesia used.

The insulin-like growth factors (IGFs) signaling system is one of the key pathways that regulating the growth, development, and aging of the body. The signaling of IGFs is regulated by the insulin-like growth factor-binding proteins (IGFBPs). IGFBP5, as a member of the IGFBPs family, has the most conserved sequences among the members of the IGFBPs family across species yet, and which has the most diverse biological roles. The expression level and the functional role of IGFBP5 may differ in the different contexts. The results of several previous studies show that the elevated level of IGFBP5 may reduce the reproductive capacity of the body. However, the results of the current studies on the influence of the elevated IGFBP5 level of the body on their disorders of glucose-lipid metabolism and their development of diabetic complications are all still highly contradictory. In conclusion, IGFBP5 is a multifunctional protein with the strong background specificity in its effect and mechanism of action, which is capable of the exerting positive or negative regulatory effect in a specific space and time, through different pathways.

To exploration of the gene detection for the instructional aspirin precision medicine for patients with immune recurrent spontaneous abortion. Methods: 50 patients with recurrent spontaneous abortion who had treated in hospital between June 2022 and December 2023 were collected in this study. The genetic testing based on blood samples of these patients were conducted before their aspirin administration, and the medication regimen of aspirin was adjusted according to the genetic testing results. Results: Genetic testing was performed on 50 patients with recurrent spontaneous abortion. Three genotypes GG, GA, and AA of the aspirin resistance genes CYP2C19 and CHIA were detected, and three genotypes AC, CC, and AA of gene LTC4S were detected. Two genotypes CC and CT of drug efficacy gene GP1BA were detected. All the genotypes detected of gene PTGS1 were AA. Based on the gene test results, the subsequent guidance of aspirin administration was provided to the patients. Among 50 patients with aspirin used, 21 cases had changed their treatment regimens, and 3 cases had continual their treatment regimens but required close monitoring of their drug adverse reactions. Conclusion: Aspirin pharmacogenomic testing for the patients with recurrent miscarriage can precisely guide their clinical drug used.

To explore the effects of the Guchong decoction plus or minus for treating patients with abnormal uterine bleeding on the improvement of their clinical symptoms and prognosis. Methods: A total of 102 patients with abnormal uterine bleeding who received outpatient treatment were selected and were divided into two groups (51 cases in each group) by bicolor sphere method from January 2021 to September 2023. The patients in the two groups were given dydrogesterone tablets for 6 menstrual cycles, and the patients in the study group were given Guchong decoction plus or minus additionally. The patients in both groups received 6 months of follow-up. The efficacy, the levels of the follicle stimulating hormone (FSH), estradiol (E2) and progesterone (P), the endometrial thickness, the rates of adverse reactions and abnormal uterine bleeding recurrence, and the prognostic quality of life of the patients were compared between the two groups. Results: The efficient rate (94.1%) of the patients in the study group after treatment was significantly higher than that (78.4%) of the patients in the control group. The E2 level of the patients in both groups had increased significantly and the levels of FSH and P of the patients in both groups had decreased significantly, and the variations of which of the patients in the study group were significantly more than those of the patients in the control group. The endometrium thickness of the patients in both groups had decreased significantly, and which (0.78±0.19cm) of the patients in the study group was significantly lower than that (1.09±0.24cm) of the patients in the control group. The incidence of adverse reactions (9.8%) of the patients in the study group was significantly lower than that (27.5%) of the patients in the control group, and the score of the Chinese version of world health organization quality of life scale (WHOQOLBREF) of the patients in both groups had increased significantly, and which (90.10±1.73 points) of the patients in the study group was significantly higher than that (84.56±2.95 points) of the patients in the control group. The recurrence rate of abnormal uterine bleeding (6.0%) of the patients in the study group was significantly lower than that (23.5%) of the patients in the control group (all P<0.05). Conclusion: Guchong decoction plus or minus for treating patients with abnormal uterine bleeding can significantly improve the clinical therapeutic effect, the sex hormone levels and the endometrial thickness, increase the prognosis quality of life and reduce the recurrence rate of abnormal uterine bleeding of the patients.

To explore the value of the levels of the serum ferritin (SF) and transferring (TRF) of infertile patients with obese polycystic ovary syndrome (PCOS) for evaluating their fertility outcomes after in vitro fertilization-embryo transfer (IVF-ET). Methods: The clinical data of 78 infertile patients with PCOS who visited the hospital from August 2021 to December 2023 were selected as research subjects. Based on the body mass index (BMI) of the patients, these patients were divided into group A (48 cases with obese) and group B (30 cases without obese). All these patients were given the treatment of IVF-ET, and these patients were divided into two groups, including 28 cases with failure of pregnancy in group C and 50 cases with pregnancy success in group D. The levels of SF and TRF of the infertile patients were measured by immunoturbidimetric method. The homeostatic model assessment for insulin resistance (HOMA-IR) value of the patients was calculated. The correlation between the serum SF and TRF levels of the infertile patients with PCOS and their HOMA-IR value was analyzed by Spearman correlation coefficient. Receiver operating characteristic (ROC) curve was constructed to predict the value of the serum SF and TRF levels of the infertile patients with PCOS for their pregnancy outcomes. Results: The levels of SF (7.58±1.82 ng/ml) and TRF (16.53±2.81μg/ml), and the HOMA-IR value (3.39±0.94) of the patients in group A were significantly higher than those (5.28±0.96 ng/ml, 11.65±1.53μg/ml and 2.06±0.49) of the patients in group B. Spearman correlation analysis showed that the serum SF and TRF levels of the patients were positively correlated with their HOMA-IR value. The levels of SF and TRF, and the HOMA-IR of the patients in group C were significantly higher than those of the patients in group D (all P<0.05). The area under the curve, the sensitivity and the specificity of the serum SF level combined with the TRF level of the infertility patients with PCOS for predicting their pregnancy failure were 0.925, 82.1% and 90.0%, respectively. Conclusion: The levels of the serum SF and TRF of the infertility patients with PCOS are abnormally increased, and the combination of the SF and TRF levels has the higher efficacy for predicting the outcomes of IVF-ET of the patients.

To investigate the effect of Bushenhuoxue prescription for treating patients with polycystic ovary syndrome (PCOS), and to study its improvements of hormone and ovarian function of the patients. Methods: 120 infertility patients with PCOS admitted to hospital from January 2021 to October 2023 were selected and were divided into experimental group and control group according to the double-blind method, with 60 cases in each group. The patients in the control group were treated with clomiphene citrate tablets for 3 menstrual cycles, and the patients in the experimental group were treated with Bushenhuoxue prescription for 3 menstrual cycles. The endometrial receptivity, the follicle development situation, the hormone level, the ovulation rate, and the pregnancy outcomes of the patients in the two groups after treatment were observed. Results: After treatment, the ovulation rate (93.3%) and the pregnancy rate (55.0%) of the patients in the experimental group were significantly higher than those (80.0% and 33.3%) of the patients in the control group, but the spontaneous abortion rate (3.3%) of the patients in the experimental group was lower than that (16.7%) of the patients in the control group. The endometrial thickness (9.36±0.77 mm) of the patients in the experimental group was significantly higher than that (8.42±0.59 mm) of the patients in the control group. The values of uterine artery pulsatility index (2.02±0.21) and uterine artery resistance index (0.59±0.08) of the patients in the experimental group were significantly lower than those (2.31±0.26 and 0.71±0.11) of the patients in the control group. The largest follicle diameter (2.23±0.42 cm) and the number of dominant follicle (2.68±0.51) of the patients in the experimental group were significantly higher than those (1.92±0.40 cm and 2.23±0.42) of the patients in the control group. The number of follicles (19.85±3.98) of the patients in the experimental group was significantly less than that (24.28±4.06) of the patients in the control group. The levels of follicle-stimulating hormone (3.77±0.86 U/L), luteinizing hormone (8.11±2.21 U/L) and testosterone (0.51±0.07 ng/ml) of the patients in the experimental group were significantly lower than those (4.61±0.79 U/L, 9.95±2.61 U/L and 0.64±0.14 ng/ml) of the patients in the control group, but the progesterone level (0.89±0.09 nmol/L) of the patients in the experimental group was significantly higher than that (0.72±0.04 nmol/L) of the patients in the control group (all P<0.05). Conclusion: Bushenhuoxue prescription for treating patients with PCOS is helpful to increase their receptivity of endometrium, to promote their normal development of follicle and to correct sex hormone disorder, to increase their ovulation rate and to improve their pregnancy outcomes, and which has good clinical efficacy.

To explore the correlation between the marital support and health literacy of patients with cervical cancer and their fear of cancer recurrence, and to provide reference for the clinical nursing of the patients. Methods: A total of 206 patients with cervical cancer who had undergone surgical treatment in hospital from July 2021 to June 2023 were investigated by the general information questionnaire, the couple support questionnaire (CSQ), the health literacy scale for patients with chronic diseases (HeLMS) and the fear of progression simplified scale (FoPQSF). Pearson regression analysis was used to analyze the correlation between the scores of CSQ and HeLMS of the patients and their FoPQSF score. Multiple linear regression analysis was used to identify the related factors affecting the fear of cancer recurrence of the patients. Results: A total of 206 questionnaires were distributed, and 189 were effectively recovered, with an effective recovery rate of 91.8%. The FoPQSF score of the patients with cervical cancer was 39.52±6.74 points, of which, the score of 105(55.6%) cases was ≥34 points. The scores of CSQ and HeLMS of the patients with cervical cancer were 68.6±10.59 points and 65.77±9.56 points. Pearson regression analysis showed that the CSQ score and HeLMS score of the patients with cervical cancer were negatively correlated with their FoPQSF score (P<0.001). Multivariate analysis showed that the young age, the low family per capita monthly income, the advanced tumor stage, and the low scores of CSQ and HeLMS of the patients with cervical cancer were all the factors influencing their cancer recurrence fear (all P<0.001). Conclusion: The incidence and the level of cancer recurrence fear of the patients with cervical cancer are all high. The marital support and the health literacy of patients with cervical cancer are negatively correlated with their fear of cancer recurrence. The nursing intervention measures should be taken according to these influencing factors of the cancer recurrence fear of the patients with cervical cancer in order to reduce their fear of cancer recurrence and improve their treatment effect.

To analysis the self-efficacy correlation factors and countermeasure of neonatal father support breastfeeding. Methods: From March 2021 to January 2024, neonatal 170 fathers in hospital were selected as the study subjects, and a questionnaire survey was conducted on these fathers before the discharge of the neonates. The general data of the neonatal father and mothers were collected. The comprehensive breastfeeding knowledge scale (CBKS), the Iowa infant feeding attitude scale (IIFAS) and the short father breastfeeding support self-efficacy scale form (FBSES-SF) were used to assesses the knowledge of breastfeeding, attitudes towards infant feeding and self-efficacy in breastfeeding support of the fathers. Pearson analysis was used to analyze the correlation between the infant feeding attitudes and the breastfeeding knowledge level of the neonatal fathers and their self-efficacy in support of breastfeeding. Multiple linear regression analysis was used to analyze the factors affecting the self-efficacy in support of breastfeeding of the neonatal fathers. Results: 170 neonatal fathers filled in the questionnaire, and the qualified rate was 95.3% (162/170). The scores of FBSES-SF, IIFAS  and CBKS of 162 fathers were 49.82±7.14 points, 49.02±5.24 points and 61.14±6.58 points. Univariate analysis showed that the educational level, the couple’s parity, the type of neonates and the neonatal feeding pattern of the fathers before the neonatal discharge were related to their self-efficacy in supporting breastfeeding (P<0.05). There was a positive correlation between the infant feeding attitude and the knowledge level of breastfeeding of the neonatal fathers and their self-efficacy in supporting breastfeeding (P<0.05). The low education level, the parity of one, the low infant feeding attitude and the low knowledge level of breastfeeding of the neonatal fathers, and the feeding pattern without breastfeeding before discharge were the influencing factors of the self-efficacy in supporting breastfeeding of the neonatal fathers (all P<0.05). Conclusion: The level of self-efficacy in supporting breastfeeding of the fathers needs to be improved. The targeted intervention strategies should be conducted according to the relevant factors to improve the self-efficacy in supporting breastfeeding of the neonatal fathers in clinical practice.

To investigate the effect of the dynamic symptom management model for intervening patients with ovarian cancer in their perioperative symptom cluster management. Methods: 118 middle-aged patients with ovarian cancer who had admitted to hospitals were selected and were divided into two groups (59 cases in each group) by random number table method from January 2019 to December 2023. The patients in the two groups were given routine nursing and symptom management records, and the patients in the experimental group were given the intervention of the dynamic symptom management model additionally. The interventions of the patients in the two groups were conducted at 4 time points, including at 1-3 days before surgery (T1), and at the 1st day after surgery (T2), 2nd-3rd day after surgery (T3) and 5th day after surgery (T4). The differences of the symptoms with different severity situation at each time point and the differences of the quality of life of the patients were compared between the two groups. Results: There were significant differences in the time effect, the group effect and the interaction effect of the symptom score of the patients after intervention between the two groups (P<0.05). The evaluations of the society function, the role function, the cognitive function, the emotional function and the general function of the patients in the experimental group after intervention had increased significantly, but the scores of fatigue, pain, loss of appetite, diarrhea and respiratory difficulties of the patients in the experimental group after intervention had decreased significantly. The evaluations of the cognitive function, the emotional function and the general function of the patients in the experimental group were significantly higher than those of the patients in the control group. The scores of fatigue, pain and loss of appetite of the patients in the experimental group after intervention were significantly lower than those of the patients in the control group (all P<0.05). Conclusion: The dynamic symptom management model in the perioperative symptom cluster management of the patients with ovarian cancer has better interventive effect, and which can improve the quality of life and promote the recovery of the patients.

To understand the current situation of the breastfeeding of women within postpartum 6 months in Minhang district of Shanghai, and to explore the relevant factors affecting the breastfeeding of the women. Methods: 836 women within postpartum 6-10 months from Minhang district between February and June 2023 were selected as the respondents in this investigation. The breastfeeding situation of the women was investigated and the relevant factors affecting breastfeeding of the women were analyzed. Results: 814 women were investigated effectively. The breastfeeding rate of the women within postpartum 6 months was 82.9% (675/814) and the exclusive breastfeeding rate of them was 41.4% (337/814) at. Multivariate logistic regression analysis showed that the age of 21-25 years old, 26-30 years old and ≥35 years old (OR=2.446, 2.258 and 2.341, 95%CI 1.106-5.409, 1.203-4.240 and 1.135-4.831) and the breastfeeding (OR=3.837, 95%CI 2.517-5.849) and the no work after delivery (OR=2.785, 95%CI 1.795-4.323) of the women within postpartum 6 months were the promoting factors for their exclusive breastfeeding, while the gestational diabetes mellitus (OR=0.573, 95%CI 0.347-0.946) of the women within postpartum 6 months was the risk factor of their exclusive breastfeeding. Conclusion: In order to increase the breastfeeding rate, especially the exclusive breastfeeding rate of the women within postpartum 6 months, the social support should be given to these women. And the individualized guidance should be given to the women within postpartum 6 months after delivery according to their physiological characteristics and their special situations faced.

To observe the effects of pelvic floor biomimetic electrical stimulation therapy for patients with thin endometrium infertility on their endometrial receptivity and pregnancy outcomes. Methods: 8 patients with mesosterenotic endometrial infertility were randomly divided into observation group (40 cases given conventional hormone therapy combined with pelvic floor biomimetic electrical stimulation) and control group (40 cases given conventional hormone therapy). The patients in both groups were treated for 3 courses of treatment (1 menstrual cycle as 1 course). The endometrial status, such as the endometrium type, the endometrial thickness (EMT) and the endometrial volume, the values of uterine artery hemodynamic parameters, such as pulsatility index (PI), resistance index (RI) and peak systolic velocity/end diastolic velocity (S/D), and the levels of sex hormones of the patients before treatment and after 3 courses of treatment were compared between the two groups. The pregnancy status of the patients in the two groups within 1 year after the end of treatment was statistically analyzed. Results: After 3 courses of treatments, the proportion (67.5%) of type A endometrium, the EMT (9.48±0.65mm) and the endometrial volume (2.34±0.38 cm2) of the patients in the observation group were significantly higher than those (32.0%, 8.23±0.79mm and 1.71±0.36 cm2) of the patients in the control group, and the levels of serum estradiol (E2) and progesterone (P) of the patients in the observation group were significantly higher than those of the patients in the control group, while the values of uterine artery hemodynamic parameters and the levels of the luteinizing hormone (LH), testosterone (T), folliclestimulating hormone (FSH) and prolactin (PRL) of the patients in the observation group were significantly lower than those of the patients in the control group. The pregnancy rate (42.5%) of the patients in the observation group was significantly higher than that (20.0%) of the patients in the control group (all P<0.05). Conclusion: Pelvic floor biomimetic electrical stimulation adjuvanting the hormone therapy for the patients with thin endometrium infertility can effectively enhance their endometrial receptivity and improve their hemodynamics and sex hormones levels, and it has a positive effect on the improvement of the pregnancy rate of the patients.

To explore the status and the influencing factors of the postpartum pelvic floor dysfunction (PFD) of women after vaginal delivery. Methods: 190 women who had successfully undergone vaginal delivery in the hospital from February 2019 to October 2023, and had PFD screened in the 42th day after delivery were selected in this study. According to the presence or absence of PFD, these women were divided into group A (women with PFD) and group B (women without PFD). The maternal clinical and delivery data, the laboratory indicators of the women in the two groups were collected. Univariate and logistic multivariate regression analysis were used to determine the PFD high-risk factors of the women after vaginal delivery. Results: The incidence of PFD of the women after vaginal delivery was 35.8%. Univariate analysis showed that there were significant differences in the age, the gestational weeks, the labor analgesia situation, the second stage of labor, the episiotomy situation, the neonatal weight and the 25-hydroxyvitamin D level of the women after vaginal delivery between group A and group B. Multi-factor analysis showed that the advanced age, the more gestational weeks, the long second stage of labor, the episiotomy and the high neonatal weight of the women were the independent risk factors of their postpartum PFD after vaginal delivery, and the high 25 hydroxy vitamin D level of the women was their protection factor of the postpartum PFD after vaginal delivery (all P<0.05). Conclusion: The incidence of the postpartum PFD of the women after vaginal delivery is still high, and the age ≥35 years old, >40 gestational weeks, the duration of the second stage of labor >1.5h, the episiotomy, the neonatal weight >3.5 kg and the low 25-hydroxyvitamin D level of the women are the risk factors of their postpartum PFD, so which should be great paid attention to in clinic, and the target nursing intervention measures should be conducted to promote the physical and mental health of the postpartum women.

To investigate the effects of the different hemostatic methods during the laparoscopic ovarian cystectomy of patients with ovarian cysts on their immunity and ovarian reserve function. Methods: A total of 56 patients with ovarian cysts who wanted laparoscopic ovarian cystectomy were selected in this study from October 2022 to October 2023. The patients who had received electrocoagulation hemostasis during the operation were in group A and 56 patients who had received suture hemostasis during the operation were in group B. The operation related indexes, the levels of body immunity function indexes, such as CD8+T, CD69 and CD30, the ovarian stromal blood flow and the reserve function situation of the patients were compared between the two groups. Results: The operation time (54.1±2.3 min) and the intraoperative blood loss (41.1±2.7 ml)  of the patients in group A were significantly lower than those  (68.1±2.1 min and 49.9±3.1 ml) of the patients in group B (all P<0.05). There was no significant difference in the hospitalization time of the patients between the two groups (P>0.05). 1 day after operation, the level of CD8+T of the patients in the two groups had decreased significantly, and the levels of CD69 and CD30 of the patients in the two groups had increased significantly, but the changes of the patients in group A were significantly lower than those of the patients in group B. Three months after operation, the values of ovarian vascular matrix pulsatility index (1.26±0.10) and the resistance index (0.78±0.07) of the patients in group A were significantly higher than those (1.19±0.22 and 0.74±0.13) of the patients in group B. The ovarian vascular minimum diastolic flow velocity value (2.57±0.18 cm/s) of the patients in group A was significantly lower than that (2.93±0.31 cm/s) of the patients in group B. The levels of serum follicle-stimulating hormone and luteinizing hormone of the patients in group A were significantly higher than those of the patients in group B, and the serum estradiol level of the patients in group A was significantly lower than that of the patients in group B. The peak velocity value of ovarian vascular stromal blood flow (9.04±1.04 cm/s) and the number of ovarian antral follicles (5.57±0.68 eggs) of the patients in group A were significantly lower than those (10.98±1.01 cm/s and 7.13±0.55 eggs) of the patients in group B (all P<0.05). Conclusion: Compared with those of the suture hemostasis, the electrocoagulation hemostasis during laparoscopic ovarian cystectomy of the patients has shorter operation time, less intraoperative blood loss and less influence on the immune function of the patients, but which has more influence on the ovarian stromal blood flow and reserve function of the patients.

To explore the impacts of the traditional Chinese medicine acupoint massage combined with freeposition during the stages of labor of primiparous with vaginal delivery on their duration of labor and postpartum bleeding volume. Methods: A total of 120 primiparous who wanted vaginal delivery were selected and were randomly divided into two groups from June 2021 to June 2023. The primiparous in the control group were given conventional delivery mode, while the primiparous in the study group were given traditional Chinese medicine acupoint massage combined with free position delivery mode. The duration of labor and the postpartum bleeding volume of the primiparous were compared between the two groups. Results: The rate of vaginal delivery (91.7%) of the primiparous in the study group was significantly higher than that (75.0%) of the primiparous in the control group. The first stage of labor duration (356.4±69.0 min), the second stage of labor duration (34.5±10.8 min) and the third stage of labor duration (6.1±1.1 min) of the primiparous in the study group were significantly shorter than those (442.2±82.6min, 46.1±14.5min and 9.6±1.5min) of the primiparous in the control group. The proportion of the pain degree ≤2 grade during delivery (90.0%) and the delivery control score (178.41±19.68 points) of the primiparous in the study group were significantly higher than those (68.3% and 121.88±16.54 points) of the primiparous in the control group. The amount of postpartum hemorrhage (208.4±30.0ml) and the incidence of complications during delivery (11.7%) of the primiparous in the study group were significantly lower than those (292.5±37.3ml and 31.7%) of the primiparous in the control group (all P<0.05). Conclusion: the traditional Chinese medicine acupoint massage combined with freeposition during the stages of labor of the primiparous can reduce their cesarean section rate, shorten their stages of labor in vaginal delivery, alleviate their labor pain, reduce their postpartum bleeding, and decrease their complications during delivery.

To compare the anesthesic effect and the postoperative analgesia of the epidural anesthesia and the combined lumbar and epidural anesthesia during caesarean section of women. Methods:  92 pregnant women who wanted caesarean section were selected and were divided into control group (45 women with epidural anesthesia) and experimental group (47 women with combined epidural anesthesia) according to the anesthesia method during caesarean section from March 2021 to March 2023. The perioperative indexes values, the intraoperative anesthesia effect, the score of visual analogue scale (VAS) at postoperative 2h (T1), postoperative 5h (T2), postoperative 12h (T3), postoperative 1d (T4) or postoperative 2d (T5), the sedation score (Ramsay) and the adverse reactions rate of the women were compared between the two groups. Results: The operation time, the anesthesia time, and the fetal delivery time of the women in the experimental group were significantly shorter than those of the women in the control group. The proportion of the auxiliary drugs (42.6%) of the women in the experimental group was significantly lower than that (69.9%) of the women in the control group. The total effective rate of anesthesia (97.9%) of the women in the experimental group was significantly higher than that (86.7%) of the women in the control group. The VAS scores of the women in the experimental group at T1, T2, T3, T4 and T5 (5.43±1.01 points, 4.01±1.12 points, 3.81±1.23 points, 2.18±0.11 points and 1.46±0.12 points) were significantly lower than those (6.93±1.25 points, 5.96±1.91 points, 4.96±1.74 points, 2.78±0.38 points and 1.79±0.6 5 points) of the women in the control group. The postoperative Ramsay score of the women in the two groups had increased significantly, and which of the women in the experimental group at T1, T2, T3, T4 or T5 was significantly higher than that of the women in the control group. The incidence of adverse reactions (17.0%) of the women in the experimental group was significantly lower than that (42.2%) of the women in the control group (all P<0.05). Conclusion: The anesthesic effect of the combined lumbar and epidural anesthesia during caesarean section of the women is better than that of the epidural anesthesia, which can relieve the pain and decrease the adverse reaction of the women after caesarean section, and with certain safety.

To investigate the application effect of Thinprep cytology test (TCT) combined with high-risk human papillomavirus (HR-HPV) detection for screening cervical cancer. Methods: The clinical data of 3,500 women who received cervical cancer screening from January 2022 to May 2023 were selected as the study subjects. With the pathological diagnosis as the gold standard, the diagnostic efficacy of TCT, HR-HPV detection, and the combination of TCT and HR-HPV detection of the women for their cervical cancer was analyzed by receiver operating characteristic (ROC) curve. Results: Among 3500 women, there were 33 cases with cervical cancer, 112 cases with cervical intraepithelial neoplasia (CIN)II and CINI II, 267 cases with CIN I and 3,088 cases with cervicitis. ROC curve analysis results showed that the area under the curve (AUC) of the TCT of the women for screening their cervical cancer was 0.741, with the sensitivity of 48.5% and the specificity of 99.7%. The AUC of the HR- HPV detection of the women for screening their cervical cancer was 0.769, with the sensitivity of 54.6% and the specificity of 99.3%. When the women diagnosed as cervical cancer based on the TCT or the HR-HPV detection with positive result, the AUC of the TCT combined with the HR-HPV detection of the women for screening their cervical cancer was 0.980, with the sensitivity of 97.0% and the specificity of 99.1%. Conclusion: TCT combined with HR-PHV detection of the women for screening their cervical cancer has higher diagnostic efficacy, which can improve the diagnostic sensitivity and has higher clinical value for screening the cervical cancer and the precancerous lesions of the women.

To explore the predictive value of the combined levels of serum human epididymal secretion protein 4 (HE4), transmembrane 4-L six-family-1 (TM4SF1) and heat shock protein 90α (HSP90α) of women with ovarian cancer (OC) for their postoperative recurrence. Methods: The clinical data of 122 women with OC (in group A) who had undergone surgical treatment in the hospital from October 2018 to December 2020 were selected in this study, and these women were divided into group A1 (85 women without OC recurrence) and group A2 (37 women with OC recurrence) according to the postoperative recurrence situation of these women within 3 years of follow up after surgery. 102 women with benign ovarian tumor who also had been treated in the hospital during the same period were included in group B, and 115 healthy women who had undergone physical examinations in the hospital were included in group C. The levels of serum HE4, TM4SF1, and HSP90α of the women were compared among group A, group B and group C. Logistic regression was applied to analyze the influencing factors of the postoperative OC recurrence of the women in group A. Receiver operating characteristic (ROC) curve was applied to analyze the predictive values of the levels of serum HE4, TM4SF1, and HSP90αof the women in group A for their postoperative OC recurrence. Results: The levels of serum HE4 (158.49±41.27 pmol/L), TM4SF1 (171.28±42.33 ng/ml) and HSP90α(79.22±15.38 ng/ml) of the women in group A were significantly higher than those (34.53±8.39 pmol/L, 111.18±25.46 ng/ml and 58.19±10.24 ng/ml) of the women in group B and those (33.31±8.12 pmol/L, 102.37±22.39 ng/ml and 56.22±9.27 ng/ml) of the women in group C. The serum HE4, TM4SF1, HSP90αlevels of the women in group A2 were significantly higher than those of the women in group A1 (all P<0.05), while which of the women in group B had no significantly different from those of the women in group C (P>0.05). Logistic regression analysis showed that the FIGO stage III, poor differentiation and lymph node metastasis of OC, and the increased levels of serum HE4, TM4SF1 and HSP90α of the women with OC were the influencing factors of their postoperative OC recurrence. ROC curve analysis showed that the area under curve (AUC) of the HE4 level, the TM4SF1 level, and the HSP90α level of the women with OC for predicting their postoperative OC recurrence were 0.874, 0.828 and 0.872, respectively, the sensitivity of which were 89.2%, 89.2% and 89.2%, respectively, and the specificity of which were 66.8%, 69.2% and 63.3%, respectively. The AUC, the sensitivity and the specificity of the combined levels of serum HE4, TM4SF1 and HSP90αof the women with OC for predicting their postoperative OC recurrence were 0.958, 86.5%, and 78.3%, respectively, and the AUC of the combined levels of serum HE4, TM4SF1 and HSP90α of the women with OC for predicting their postoperative OC recurrence was the best (P<0.05). Conclusion: The levels of serum HE4, TM4SF1, and HSP90α of the women with the postoperative OC recurrence all elevate abnormally, and which are the risk factors of the postoperative OC recurrence of the women. The combined levels of serum HE4, TM4SF1 and HSP90α of the women with OC for predicting their postoperative OC recurrence has certain value.

To analyze the effects of the cesarean section or the vaginal delivery of pregnant women with the premature delivery during different gestational weeks on the maternal and infant outcomes. Methods: The clinical data of 300 pregnant women with the premature delivery during the gestational weeks of 28 weeks to 36+6 weeks in the hospital from January 2019 to December 2021 were analyzed retrospectively. Among the women with the premature delivery during the different gestational weeks, 50 cases with cesarean section were selected in group A and 50 cases with vaginal delivery were selected in group B randomly. The difference of the maternal and infant outcomes was compared between the women with cesarean section and the women with vaginal delivery. Results: The incidences of the neonatal respiratory distress syndrome (NRDS) and the complications during pregnancy, and the proportions of the number of complications ≥3 and the number of preterm neonatal complications ≥2 of the women delivered during 28-31+6 gestational weeks,  of the women delivered during 32-33+6 gestational weeks and of the women delivered during 34-36+6 gestational weeks had decreased gradually, and the neonatal birth weight of these women had increased gradually (all P<0.05). The neonatal mortality rate of the women delivered during 34-36+6 gestational weeks was significantly lower than that of the women delivered during 28-31+6 gestational weeks (P<0.05). Among the women delivered during 28-31+6 and 34-36+6 gestational weeks, there were no significant differences in the age, the body mass index (BMI), the proportion of parity ≥2 times, the incidences of the complications during pregnancy and postpartum hemorrhage, the neonatal birth weight, and the incidences of the neonatal death, NRDS and complications the women between group A and group B (P>0.05). For the pregnant women who delivered during 32-33+6 gestational weeks, there were no significant differences in the age, the BMI, the proportion of parity ≥2 times, the pregnancy complications rate, the neonatal birth weight and the incidence of neonatal death of the women between group A and group B (P>0.05). The incidence of the neonatal NRDS and the neonatal complications number≥2 of the women in group A were significantly lower than those of the women in group B (P<0.05). Conclusion: The cesarean section and the vaginal delivery of the preterm delivery women during different gestational weeks have different effects on the maternal and neonatal outcomes. The cesarean section of the women delivered during 32-33+6 gestational weeks may reduce the risk of their neonatal complications.

To explore the effects of high-risk human papillomavirus (HR-HPV) infection of the patients on their cervical and vaginal microecology and the expressions of miR-149-3p and human cytochrome b reductase 1 (CYBRD1) in their cervical exfoliated cells. Methods: The clinical data of 102 patients who were admitted to the hospital due to cervicitis from December 2020 to December 2022 were selected in this study. These women were divided in to group A (65 cases without HPV infection）and group B (37 cases with HPV infection), and the HPV infection subtypes of the patients in group B were detected. The vaginal microecological changes and the vaginal microecological balance of the patients were compared between the two groups. The levels of miR-149-3p and CYBRD1 in the cervical exfoliated cells of the patients in the two groups were detected by fluorescence quantitative PCR. Spearman method was used to analyze the correlation between the cervical vaginal microecology of the patients and their miR-149-3p and CYBRD1 expressions in the cervical exfoliated cells. Multi-factor logistic stepwise regression analysis was used to explore the influence factors of the HR-HPV infection of the patients. Results: The severity of cervical lesions of the patients in group B was significantly higher than that of the patients in group A. In group B, the subtype HPV16 detection rate was the highest (51.0%), and then followed by HPV18 (18.6%). The vaginal microecological imbalance, the positive rate of ureaplasma urealyticum and the abnormal rate of lactobacillus of the patients in group B were significantly higher than those of the patients in group A. The expression of miR-149-3p (0.53±0.12) of the patients in group B was significantly lower than that (1.67±0.23) of the patients in group A, and the relative expression of CYBRD1 of the patients (1.29±0.27) in group B was significantly higher than that (0.46±0.10) of the patients in group A (all P<0.05). The HR-HPV infection of the patients showed the negative correlation with the miR-149-3 P expression in their cervical exfoliated cells, and was positive correlation with their CYBRD1 expression, vaginal microecological imbalance, rate of positive of ureaplasma urealyticum and abnormal rate of lactobacillus (all P<0.05). The proportion of the adverse pregnancy and childbirth history of the patients in group B was significantly higher than that of the patients in group A (P<0.05). Multivariate analysis showed that the positive rate of ureaplasma urealyticum, the abnormal rate of lactobacillus, the adverse pregnancy and childbirth history, the vaginal microecological imbalance, the decreased miR-149-3p level and the increased CYBRD1 level of the patients were the influencing factors of their HR-HPV infection (all P<0.05). Conclusion: The patients with HR-HPV infection have the low expression of miR-149-3p and the high expression of CYBRD1 in their cervical exfoliated cells, and HPV16 and HPV18 are the main subtypes HPV infection of the patients with HR-HPV infection. Moreover, HR-HPV infection of the patients will affect their imbalance of cervical and vaginal microecology, and will increase the severity of their cervical lesions.

To understand the willingness of primiparas to choose the analgesia delivery, and to analyze its related influencing factors. Methods: A total of 198 primiparas admitted to the hospital from October 2020 to February 2024 were selected as the study subjects. The general information questionnaire was used to investigate these primiparas at their last prenatal examination. According to whether the primiparous had the willingness to choose analgesia delivery or not, these primiparas were divided into the group A (primiparas with the willingness to choose analgesia delivery) and group B (primiparas without the willingness to choose analgesia delivery). The fear of childbirth scale (CAQ) was used to understand the fear of childbirth of the primiparas. The pain threshold of the primiparas was measured by hand-held pressure gauge. Univariate and multivariate logistic regression analysis were used to determine the related influencing factors of the willingness to choose analgesia delivery of the primiparas. Results: A total of 198 questionnaires were distributed, and 187 questionnaires were effectively recovered, with the effective recovery rate of 94.4%. Among 187 primiparas, 92 (49.2%) primiparas had the willingness to choose analgesia delivery before delivery, and 95 (50.8%) primiparas had no the willingness to choose painless labor before delivery. Multivariate logistic regression analysis showed that the higher education level, the occupation and the spouse 's consent of the primiparas had the positive impacts on their willingness to choose analgesic delivery, and the high pain threshold of the primiparas had the negative impact (all P<0.05). Conclusion: There is still much room for the improvement on the choice rate of the analgesia delivery intention of the primiparous. The factors affecting the choice intention of the analgesia delivery of the primiparous may be their education level, occupation, spouse’s consent and pain threshold, so the clinical measures should be taken as soon as possible to promote the application of analgesia delivery of the primiparas.

To explore the comparative effect of nasal intermittent positive pressure ventilation (NIPPV) and synchronized intermittent mandatory ventilation (SIMV) for treating the premature infants with neonatal respiratory distress syndrome (NRDS). Methods: 102 premature infants with NRDS were selected and were divided into group A (51 infants with NIPPV) and group B (51 infants with SIMV) by random number table method from June 2021 to June 2023. The clinical efficacy after 3 days of treatment, the clinical related indicators values, the values of blood gas indicators, such as partial pressure of carbon dioxide (PaCO2), partial pressure of oxygen (PaO2) and arterial oxygen saturation (SaO2), the levels of inflammatory indicators, such as interleukin-6 (IL-6),  high mobility group protein-1 (HMGB-1), tumor necrosis factor-α (TNF-α), and bone morphogenetic protein-7 (BMP-7), and the values of respiratory mechanics indicators, such as tidal volume (VT), dynamic lung compliance (CD) and oxygenation index (OI), of the infants before and after treatment were compared between the two groups. The incidence of the adverse reactions of the infants in the two groups during treatment was recorded. Results: The clinical efficacy (88.2%) of the infants in group A after 3d of treatment was significantly higher than that (70.6%) of the infants in group B. The breathing machine use time, the oxygen cure time, the time of hospital stay, the apnea number of the infants in group A were significantly lower than those of the infants in group B. The PaCO2 value and the levels of IL-6, HMGB-1, TNF-αand BMP-7 of the infants in the two groups had decreased significantly, and which of the infants in group A were significantly lower than those of the infants in group B. The PaO2 and SaO2 values, and the VT, CD and OI levels of the infants in the two groups had increased significantly, and which of the infants in group A were significantly higher than those of the infants in group B. The incidence of adverse reactions (11.8%) of the infants in group A was significantly lower than that (29.4%) of the infants in group B (all P<0.05). Conclusion: NIPPV for treating premature infants with NRDS has better efficacy and can significantly improve the blood gas indicators, enhance the respiratory function, inhibit the inflammatory response and reduce the risk of complications of the infants.

To systematically evaluate the effect of the percutaneous high-frequency electrical stimulation (PHFES) of the Neiguan point for the prevention and treatment of the hypotension of women during cesarean section. Methods: The databases, such as PubMed, Embase, Web of science, Cochrane Library, CNKI, Wanfang Data, VIP and CBM, were searched by computer. The randomized controlled trials (RCTs) on the effect of PHFES of the Neiguan point for the prevention and treatment of the hypotension of the women during cesarean section were included, and the quality of the included literatures was evaluated. The statistical analysis was performed using RevMan5.4 software. Results: 9 RCTs with 826 women were included. Compared with those of the women in the control group, the incidences of the intraoperative hypotension (RR=0.32, 95%CI 0.25-0.42), the vasoconstrictive drugs used (RR=0.34, 95%CI 0.24-0.48), the dizziness (RR=0.22, 95%CI 0.10-0.47), the chest tightness (RR=0.17, 95%CI 0.10-0.30), the dyspnea (RR=0.32, 95%CI 0.18-0.56), the nausea (RR=0.26, 95%CI 0.15-0.46) and the vomiting (RR=0.23, 95%CI 0.09-0.60) of the women in the experimental group were significantly decreased. There was no significant difference in the neonatal Apgar scores (WMD=0.08, 95%CI 0.05-0.20) of the women between the two groups. Conclusion: PHFES of the Neiguan point can significantly reduce the incidence of the rates of the hypotension, dizziness, chest tightness, dyspnea, nausea and vomiting of the women during cesarean section.

 To investigate the effect of the multi-dimensional nursing based on the concept of rapid rehabilitation surgery (FTS) for patients with laparoscopic ovarian cyst removal during the perioperative period. Methods: A total of 132 patients who wanted the laparoscopic ovarian cyst removal in the hospital were included and were divided into two groups (66 cases in each group) by random number table method from November 2020 to November 2023. The patients in the control group were given routine nursing, and the patients in the observation group were given the multidimensional nursing based on the FTS concept. The pain situation at different postoperative time points, the perioperative indexes values, the postoperative complication rate and the postoperative recovery quality of the patients in the two groups were observed. Results: The visual analogue scale scores of the patients in the observation group at 6h (5.01±0.33 points), at 12h (4.03±0.41 points) and at 24h (3.17±0.65 points) after operation were significantly lower than those (5.15±0.45 points, 4.22±0.65 points and 3.65±0.82 points) of the patients in the control group. The postoperative anal exhaust time (19.34±4.11h), the ambulation time (12.45±1.25h) and the hospitalization duration (6.25±0.41d) of the patients in the observation group were significantly shorter than those (23.56±6.22h, 15.33±1.89h and 7.11±0.36d) of the patients in the control group. The incidence of the postoperative complications (6.1%) of the patients in the observation group was significantly lower than that (19.7%) of the patients in the control group. The scores of each dimension of the 40-item recovery quality scale of the patients were significantly higher than those of the patients in the control group (all P<0.05). Conclusion: The multi-dimensional nursing based on the FTS concept for the patients with laparoscopic ovarian cyst removal can effectively relieve their postoperative pain, accelerate their postoperative recovery speed, shorten their duration of the hospital stay, reduce their incidence of postoperative complications and improve their quality of postoperative recovery.