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Abstract To investigate the situation of high-risk human papilloma virus (HPV) infection, and to study positive rate of methylation of SLC5A8 and RASSF2A promoter in the cells of cervical lesions. Methods: The clinical data of 186 patients with cervical diseases from May 2018 to May 2021 were collected. These patients were divided 73 cases with cervical intraepithelial neoplasia grade I in group A, 57 cases with cervical intraepithelial neoplasia grade II in group B, and 56 cases with cervical intraepithelial neoplasia grade III in group C according to their pathological diagnosis results of cervical biopsy under colposcopy. Meantime, another 43 women with normal cervical cells were included in control group. The positive rates of HPV DNA, SLC5A8, and RASSF2A gene promoter methylation in the cervical specimens of the patients in the four groups were detected. Results: The positive rates of DNA in the cervical specimens of the patients in the control group, group A, group B, and group C were 0, 28.8%, 63.2%, and 92.9%, respectively, the positive rates of SLC5A8 in the cervical specimens of the patients in the control group, group A, group B, and group C were 0, 30.1%, 66.7%, and 89.3%, respectively, and the positive rates of RASSF2A gene promoter methylation in the cervical specimens of the patients in the control group, group A, group B, and group C were 0, 13.7%, 49.1%, and 82.1%, respectively, which all had increased gradually (all P＜0.05). Conclusion: The rates of high-risk HPV infection and SLC5A8, and RASSF2A gene promoter methylation in the cervical specimens of the patients with cervical lesions are increase abnormally, and which will increase with the progression of the cervical lesions.
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