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| Effects of the high-risk human papillomavirus infection of patients on their cervical and vaginal microecology and the expressions of miR-149-3p and human cytochrome b reductase 1 |
| Chengdu Seventh People's Hospital, Chengdu, Sichuan Province, 610044 |
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Abstract To explore the effects of high-risk human papillomavirus (HR-HPV) infection of the patients on their cervical and vaginal microecology and the expressions of miR-149-3p and human cytochrome b reductase 1 (CYBRD1) in their cervical exfoliated cells. Methods: The clinical data of 102 patients who were admitted to the hospital due to cervicitis from December 2020 to December 2022 were selected in this study. These women were divided in to group A (65 cases without HPV infection）and group B (37 cases with HPV infection), and the HPV infection subtypes of the patients in group B were detected. The vaginal microecological changes and the vaginal microecological balance of the patients were compared between the two groups. The levels of miR-149-3p and CYBRD1 in the cervical exfoliated cells of the patients in the two groups were detected by fluorescence quantitative PCR. Spearman method was used to analyze the correlation between the cervical vaginal microecology of the patients and their miR-149-3p and CYBRD1 expressions in the cervical exfoliated cells. Multi-factor logistic stepwise regression analysis was used to explore the influence factors of the HR-HPV infection of the patients. Results: The severity of cervical lesions of the patients in group B was significantly higher than that of the patients in group A. In group B, the subtype HPV16 detection rate was the highest (51.0%), and then followed by HPV18 (18.6%). The vaginal microecological imbalance, the positive rate of ureaplasma urealyticum and the abnormal rate of lactobacillus of the patients in group B were significantly higher than those of the patients in group A. The expression of miR-149-3p (0.53±0.12) of the patients in group B was significantly lower than that (1.67±0.23) of the patients in group A, and the relative expression of CYBRD1 of the patients (1.29±0.27) in group B was significantly higher than that (0.46±0.10) of the patients in group A (all P<0.05). The HR-HPV infection of the patients showed the negative correlation with the miR-149-3 P expression in their cervical exfoliated cells, and was positive correlation with their CYBRD1 expression, vaginal microecological imbalance, rate of positive of ureaplasma urealyticum and abnormal rate of lactobacillus (all P<0.05). The proportion of the adverse pregnancy and childbirth history of the patients in group B was significantly higher than that of the patients in group A (P<0.05). Multivariate analysis showed that the positive rate of ureaplasma urealyticum, the abnormal rate of lactobacillus, the adverse pregnancy and childbirth history, the vaginal microecological imbalance, the decreased miR-149-3p level and the increased CYBRD1 level of the patients were the influencing factors of their HR-HPV infection (all P<0.05). Conclusion: The patients with HR-HPV infection have the low expression of miR-149-3p and the high expression of CYBRD1 in their cervical exfoliated cells, and HPV16 and HPV18 are the main subtypes HPV infection of the patients with HR-HPV infection. Moreover, HR-HPV infection of the patients will affect their imbalance of cervical and vaginal microecology, and will increase the severity of their cervical lesions.
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