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Abstract To explore the expressions of DJ-1 and miR-20b-5p in endometriosis (EMT) tissue, and to study the correlation between the DJ-1 and miR-20b-5p levels and the level of tumor suppressor gene PTEN. Methods: The tissues of eutopic endometrium (in group A) and ectopic endometrium (in group B) from 65 patients with EMT who had undergone laparoscopic surgery were selected in this study from March 2019 to March 2021, and another normal endometrial tissues from 60 patients who had undergone uterine myomectomy were selected in group C. The protein expression levels of DJ-1 and PTEN in endometrial tissues of the three groups were detected by immunohistochemistry, and the expression levels of DJ-1, miR-20b-5p, and PTEN mRNA in endometrial tissues of the three groups were detected by real-time fluorescent quantitative PCR (qRT-PCR). Pearson correlation analysis was used to analyze the relationship between the levels of DJ-1 and miR-20b-5p and the PTEN mRNA expression level in endometrial tissues of group A and B. Results: The results of immunohistochemistry showed that the expression level of DJ-1 protein in group C, in group A, and in group B had increased gradually, while the expression level of PTEN protein in group C, in group A, and in group B had decreased gradually (all P＜0.05). PCR results showed that the mRNA expression levels of DJ-1 and Mir-20B-5p in group C, in group A, and in group B had increased gradually, while the mRNA expression level of PTEN in group C, in group A, and in group B had decreased gradually (P＜0.05). Pearson correlation analysis showed that the expression levels of DJ-1 and Mir-20B-5p mRNA were negatively correlated with the PTEN mRNA level (r=-0.716, -0.587, P＜0.01). Conclusion: The expressions of DJ-1 and miR-20b-5p in endometrial tissues of EMT are up-regulated, which lead to the occurrence of EMT by negatively regulating the expression of PTEN and enhancing the proliferation of ectopic endometrial cells.
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