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| Expression of miR-134 in human ovarian cancer cells and its possible mechanisms of drug resistance |
| Qingdao Municipal Hospital, Shandong Province，266000 |
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Abstract To analyze the expression of miR-134 in human ovarian cancer cells of paclitaxel resistance, and to study its possible mechanisms involved in paclitaxel resistance.Methods: From March 2016 to March 2020, the ovarian cancer samples of 19 patients with paclitaxel-resistant of ovarian cancer in group A and the ovarian cancer samples of 21 patients without paclitaxel-resistant of ovarian cancer in group B were included in this study.The miR-134 level in human ovarian cancer cells in the two groups were tested。The chip of Agilent Human miRNA V21.0 was used to spectral analysis of miRNA gene expression in paclitaxel resistant cells and paclitaxel sensitive cells.The gene clusters with large expression differences of miR-134 were selected, and which were verified based on the results of the chip.Bioinformatics analysis software was used to predict the potential target genes of miR-134, and part of target genes were verified by Western blot.Results: The expression level of Mir-134 in ovarian cancer skOV3-TR30 cells with paclitaxel-resistant was low.A total of 128 potential target genes of Mir134 were predicted by analysis software.The expression level of target gene c-MyC protein in ovarian cancer cells with drug-resistance was significantly higher than that in ovarian cancer cells without drug-resistance (P＜0.05).After overexpression of miR-134 in SkOV3-TR30 cells, the expression of C-MyC gene had decreased, and which protein encoded had also decreased.After low expression of miR-134 in SkOV3-TR30 cells, the expression of C-MyC gene had increased, and which protein encoded had also increased (all P＜0.05).Conclusion: MiR-134 expression in ovarian cancer cells with paclitaxel-resistance is less expressed than that in sensitive cancer cells, and which affects the paclitaxel resistance.The mechanism may be produce drug resistance by regulating the expression of c-Myc protein.
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