To explore the influence mechanism of Danggui shaoyao san on immune status and NF-κB signal pathway of model rats with chronic pelvic inflammatory disease (CPID).Methods: 40 rats were selected in this study.Among them, 10 rats were randomly selected in control group, and the other 30 rats were inoculated with mixed bacteria to establish model rat with CPID.30 model rat with CPID were divided into group A (the rate given Danggui shaoyao san), group B (the rate given azithromycin), and group C (the rate given nothing drug).The effects of Danggui shaoyao san on the levels of serum pro-inflammatory and anti-inflammatory factors of the rats were analyzed.The changes of NF-κB signal pathway protein (NF-κBp50 and NF-κBp65) in the in endometrial tissue of these rats were detected.Results: The serum levels of interleukin-6 (IL-6), IL-8, and tumor necrosis factor α (TNF-α) of the rats in group C, group A, and group B were significantly higher than those of the rats in the control group, while the serum levels of IL-2 and IL-10 of the rats in group C were significantly lower than those of the rats in the control group.The serum levels of IL-6, IL-8, and TNF-α of the rats in group A and group B were significantly lower than those of the rats in group C, and the levels of IL-2 and IL-10 of the rats in group A and group B were significantly higher than those of the rats in group C (all P＜0.05).The expressions of NF-κBp50 protein and NF-κBp65 protein in endometrial tissue of the rats in group C were significantly higher than those of the rats in the control group, group A, and group B, but there were no significant differences in the expressions of NF-κBp50 protein and NF-κBp65 protein of the rats between group A and group B (P＞0.05).Conclusion: Danggui shaoyao san can inhibit the expression of NF-κB related protein of the model rats with CPID, which can reduce the level of proinflammatory factors, and can increase the level of anti-inflammatory factors, and then improve the immune status of the rats with CPID.