|
|
|
| The expression of HAS-2 and CD44 in endometrial cells of women with adenomyosis and the effect of estrogen on the expression of HAS-2 and CD44 |
| Beijing Obstetrics and Gynecology Hospital, Capital Medical University, Beijing,100006 |
|
|
|
Abstract Objective: To explore the expression of HAS gene dominant subtypes and CD44 in cultured adenomyosis (ADS) eutopic endometrial cells in vitro, and to analyze the effect of estrogen on the expression of HAS-2 and CD44. Methods: From October 2017 to September 2018, 21 patients with ADS (ADS group) who underwent surgical treatment, and 19 patients without endometrial lesions were selected in control group. RT-qPCR was used to detect the expressions of HAS subtypes (HAS1, HAS2, HAS3) and standard CD44 of cultured endometrial cells, and also measured the responsiveness of HAS subtypes and standard CD44its by estrogen with different concentrations. Results: The expression levels of HAS2 and CD44 in endometrial cells of ADS group (0.302±0.063, 0.254±0.067, respectively) was significant higher than that (0.168±0.025, 0.128±0.018, respectively) of control group (P<0.0001), and there was a positive correlation between the expression of HAS2 and CD44 in endometrial cells of ADS group (r= 0.797, P<0.0001). There was no significant difference in the expression of HAS3 and HAS1 (P>0.05). 17 beta-estradiol in 0.1, 1.0, or 10.0 nmol/L could up-regulate the expression of HAS2 mRNA of cells in ADS group, which was significant different from that of cells without treated by 17 beta-estradiol (P<0.05), while only 17 beta-estradiol in 1.0 nmol/L could up-regulate significantly the expression of HAS2 mRNA in control group (P<0.05). 17 beta-estradiol in 1.0 and 10.0 nmol/L significantly increased the expression of CD44 mRNA in ADS group compared with that of control
group (P<0.05), while only 17 beta-estradiol in 10 nmol/L significantly increased the expression of CD44 mRNA of control group (P<0.05). Conclusion: The expression of HAS2 and CD44 in ADS endometrial cells is abnormally increased, which is regulated by high concentration of estrogen.
|
|
|
|
|
|
|
|
|
