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| A study of miR-132 promoted follicle-stimulating hormone mediated progesterone secretion of granulosa cells |
| 1.National Research Institute for Family Planning，Beijing, 100081；2. Graduate School of Peking Union Medical College |
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Abstract Objective: To explore the role of miR-132 in FSH mediated progesterone secretion in granulosa cells. Methods: Primary granulosa cells were exposed to FSH, the expression of miR-132 was detected by real-time RT-PCR, and the secretion level of progesterone was determined by radioimmunoassay (RIA). For the over expression of miR-132, Granulosa cells were infected by the recombinant adenovirus. Proteins were extracted for Western blot analysis to detect the expression of Foxo3a. Results：After the primary granulosa cells were exposed to 50ng/ml FSH, the expression of miR-132 was significantly up-regulated, and the highest expression occurred at 48 h. Compared with the cell in control recombinant adenovirus Ad-miRNC group and in blank control group, the expression level of cell in miR-132 infected by adenovirus Ad-miR132 group was significantly increased (P<0.001), and the level of progesterone secreted by granulosa cells was significantly increased after infected by Ad-miR132 (P<0.001). After FSH exposure, Foxo3a expression showed gradually decreasing, which suggested that miR-132 might negatively regulate Foxo3a expression. Conclusion: miR-132 promotes FSH mediated progesterone secretion of granulosa cells.
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