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Abstract To explore the microRNAs (miRNAs) and genes associated with asthenospermia based on bioinformatics methods, and to provide the new ideas for the diagnosis and treatment of asthenospermia. Methods: The miRNAs associated with asthenospermia were identified by screening the literatures in pubmed and Web of science, and then their target genes were predicted by TargetScan database. The data set GSE92578 related to asthenospermia selected from gene expression omnibus (GEO) and GEO2R software was used to analyze for obtaining Differential Expression Genes (DEGs). The target genes of the miRNAs and the DEGs from this analysis were intersected to obtain the final DEGs. The analysis of the gene ontology (GO) and the Kyoto encyclopedia and genomes (KEGG) genes signaling pathway enrichment of the DEGs were performed using the DAVID database. Then, the key genes were obtained by the analysis of the protein interaction (PI) network in Gene STRING database, and the node genes were obtained by cytoscape software and its plug in cytoHubba. Results: A total of 7 differentially expressed miRNAs and 84 differentially expressed genes were screened through the literature. The results of GO enrichment analysis showed that the biological processes (BP) involved by the differential genes were mainly included the homologous protein binding, the protein kinase binding and the protein serine/threonine kinase activities. The cellular components (CC) mainly included cytoplasm, cell membrane, lysosomal membrane, and acrosome, etc. The molecular functions (MF) mainly included the positive regulation of transcription from RNA polymerase II promoter, the positive regulation of protein translocation, extracellular matrix organization, and phosphorylation, etc. KEGG related pathways involved PI3K Akt signaling pathway, human papillomavirus infection pathway, platelet activation and chemoattenuation receptor activation pathways. The 10 node genes obtained by cytoscape were AKT1, MAPK3, BRD4, DNMT3A, FURIN, LMNB2, COL5A2, COL5A3, COL11A1 and COL27A1. Conclusion: The miRNAs, hub genes and the related pathways obtained in this study may play the important roles in the pathological process of the spermatogenesis of the patients, and which may provide the reference targets for the subsequent mechanistic studies on the spermatogenesis.
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