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| Research progress on the cryopreservation carriers of small quantity of human spermatozoa |
| 1.Ningxia Medical University, Yinchuan, Ningxia, 750004；2.General Hospital of Ningxia Medical University, Yinchuan；3.Key Laboratory of Fertility Preservation and Maintenance, Ministry of Education, Ningxia Medical University, Yinchuan |
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Abstract Male factors account for about 50% of global infertility, among which, the patients with azoospermia usually need to obtain the small amount of sperm through surgery, or the patients with very severe oligoasthenospermia have a small number of sperm, so it is difficult to find viable sperm after freezing resuscitation with conventional frozen carriers. Therefore, in order to avoid the cancellation of the cycle because of the unavailable sperm on the day of egg retrieval, the small number of sperm are clinically frozen by the microsperm freezing carriers in advance, such as empty egg zona, alginate capsules, micro-drop method, straws, pieces, glass denuding pipette, etc. In this paper, the research on the different cryopreservation carriers of the small quantity of the patients with spermatozoa was reviewed, so as to provide the theoretical basis for optimizing cryopreservation methods of the microsperm of the patients in clinic.
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