To analyze the analgesic effect of ultrasound-guided anterior quadratus lumborum block at the lateral supra-arcuate ligament (QLB-LSAL) and bilateral transverse abdominis plane block (TAPB) for anesthesia during total abdominal hysterectomy (TAH) of patients. Methods: 95 patients who underwent selective TAH were selected in this study from January 2022 to January 2023. According to the single-double number of the admission sequence, these patients were divided into 48 cases with QLB-LSAL for anesthesia in group A (single number of the admission sequence) and 47 cases with TAPB for anesthesia in group B (double number of the admission sequence). The heart rate (HR) and the mean arterial pressure (MAP) values of the patients at the time of entry (T0), at the skin incision (T1), at the end of surgery (T3) and at the time of leaving the anesthesia recovery room (T4), the consumption of sufentanil at 48 h after surgery, the number of patients with remedial analgesia, the number frequency of analgesic pump compression within 48h after surgery, and the scores of pain evaluated by visual analogue scale (VAS) at rest and at cough situations of the patients at 4 h, 8 h, 12 h and 24 h after surgery were compared between the two groups. The rates of postoperative adverse reactions and complications related with nerve block of the patients in the two groups were analyzed statistically. Results: The values of MAP and HR of the patients in the two groups had increased firstly and then decreased significantly, and which of the patients in group A at T1 and T2 were significantly lower than those of the patients in group B (P＜0.05). The consumption of sufentanil (69.45±4.20μg) at postoperative 48h, the rate of remedial analgesia (6.3%) and the number of analgesic pump pressing (10.5±2.3 times) of the patients in group A were significantly lower than those (73.15±4.35μg, 21.3% and 12.2±4.4 times) of the patients in group B (all P＜0.05). The postoperative VAS scores of the patients in both groups at rest and at cough showed a decreasing trend (P＜0.05), but which of the patients had no significant different at postoperative 4h and 8h between the two groups (P＞05). The scores of QLB-LSAL at rest and at cough of the patients in group A at postoperative 12h and 24h were significantly lower than those of the patients in group B (all P＜0.05). There was no significant difference in the adverse reactions rate (6.3% vs. 4.3%) of the patients between the two groups (P＞0.05). There was no any patient with nerve block occurred in the two groups. Conclusion: QLB-LSAL used for anesthesia during TAH of the patient has higher analgesic effect than that of TAPB, and which can effectively reduce the dosages of analgesic drugs used and can relieve the pain stress response of the patients.