To investigate the expression and the significance of the secretory glycoprotein 2 (Wnt2) andβ-catenin in the peripheral blood and in the umbilical cord blood of pregnant women with placenta accreta. Methods: The clinical data of 156 pregnant women with placenta accreta admitted to our hospital from May 2020 to April 2023 were selected in this study, including 78 cases with placenta accreta in group A and 78 cases with placenta implantation in group B. 80 healthy pregnant women who underwent cesarean section were selected in group C. The levels of Wnt2 and β-catenin of the women in the peripheral blood and in the umbilical cord blood were detected after admission and were compared among the three groups. Spearman correlation analysis was used to explore the correlation between the levels of Wnt2 and β-catenin of the pregnant women in the peripheral blood and in the umbilical cord blood and their placenta accreta ultrasound score. Receiver operating characteristic (ROC) curve was used to evaluate the predictive value of the levels of Wnt2 and β-catenin in the peripheral blood of the pregnant women and their placenta accreta. Results: The levels of Wnt2 and β-catenin in the peripheral blood and in the umbilical cord blood of the women in group C, in group A and in group B had increased gradually (all P<0.05). Spearman correlation analysis showed that the Wnt2 level in the peripheral blood and in the umbilical cord blood of the women with placenta implantation was positively correlated with their β-catenin level, and the levels of Wnt2 and β-catenin of the women with placenta implantation were positively correlated with their placenta accreta ultrasound rating scale score (all P<0.05). ROC curve analysis showed that the area under the curve of the Wnt2 level, the β-catenin level or the combination of the Wnt2 and β-catenin levels of the pregnant women for predicting their placenta accreta was 0.830, 0.859 or 0.905, respectively. Conclusion: The levels of Wnt2 and β-catenin in the peripheral blood and the umbilical blood of the pregnant women with placenta implantation abnormally, and which are correlated with their degree of the placenta implantation and have higher evaluation value for the placenta implantation of the pregnant women.