To investigate the clinical effect of the modified uterus-preserving iliopelvic ligament suspension for treating patients with pelvic organ prolapse. Methods: 72 patients with pelvic organ prolapse were selected and were randomly divided into observation group (36 patients treated with modified uterus-preserving laparoscopic iliopelvic ligament suspension) and control group (36 patients treated with laparoscopic abdominal wall suspension) from January 2020 to January 2022. The situations of perioperative indexes, the quantitative pelvic organ prolapse (POP-Q) index, the quality of life improvement, the pain, the complications, and the pelvic floor muscle strength of the patients in the two groups were observed. Results: The intraoperative blood loss (32.2±6.3ml) of the patients in the observation group was significantly lower than that (40.9±8.5ml) of the patients in the control group. The proportion of 0 grade of the POP-Q scale (88.9%) of the patients in the observation group after treatment was significantly higher than that (66.7%) of the patients in the control group. The quality of life score of the patients in the observation group during 1 to 6 months after treatment was significantly lower than that of the patients in the control group. The pain score of the patients in the observation group at 3h, 1d, or 2d after surgery was significantly lower than that of the patients in the control group. The total complication rate (2.8%) of the patients in the observation group was significantly lower than that (16.7%) of the patients in the control group, and the proportion of grade V of pelvic floor muscle strength (63.9%) of the patients in the observation group was significantly higher than that (25.0%) of the patients in the control group (all P<0.05). Conclusion: The modified uterus-preserving iliopelvic ligament suspension for treating patients with pelvic organ prolapsed can better improve their pelvic floor muscle strength and their quality of life.