To explore the postoperative analgesic and sedate effect of dezosin combined with butorphanol of patients after laparoscopic total hysterectomy, and to study their influences on the levels of serum high mobility group protein-1 (HMGP-1), tumor necrosis factor-α (TNF-α), and calcitonin gene-related peptide (CGRP) of the patients. Methods: 80 patients who wanted laparoscopic hysterectomy were selected and were randomly grouped into two groups (40 cases in each group) according to the treatment order from May 2021 to May 2022. The patients in the control group were treated with diazocin for analgesia, and the patients in the study group were treated with butorphanol combined with diazocin for analgesia. The scores of postoperative analgesia and sedation, the serum CGRP, TNFα, HMGp-1 levels, the operation time, the respiratory recovery time, and the adverse reactions rate of the patients were compared between the two groups. Results: The scores of analgesia and sedation of the patients in the study group at 2h, 9h, and 24h after surgery were significantly lower than those of the patients in the control group. The serum CGRP level of the patients in both groups at 48h after surgery had decreased significantly, and which (65.94±6.67 ng/L) of the patients in the study group was significantly lower than that (72.18±6.86 ng/L) of the patients in the control group. The levels of serum HMGP-1 and TNFαof the patients in both groups had increased significantly, and which of the patients in the study group were significantly lower than those of the patients in the control group. The respiratory recovery time (39.6±6.3min) of the patients in the study group was significantly shorter than that (43.5±7.3min) of the patients in the control group, and the incidence of adverse reactions (22.5%) of the patients in the study group was significantly lower than that (50.0%) of the patients in the control group (all P＜0.05). Conclusion: Dezocine combined with butorphanol used after laparoscopic hysterectomy of the patients can reduce their degree of postoperative pain, reduce their pain stress reaction, promote their respiratory recovery, and reduce the incidence of their adverse reactions.