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| Application value of human papillomavirus load detection by BMRT detection kit for screening cervical lesions |
| 1. Taixing Maternity and Child Health Care Hospital, Taixing, Jiangsu Province, 225400；2. Jiangsu Health Development Research Center, National Health Commission Contraceptives Adverse Reaction Surveillance Center, Nanjing |
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Abstract To explore the clinical value of quantitative detection of human papillomavirus(HPV)typing for screening cervical cancer. Methods: The clinical data of 204 patients with HPV positive and cytology and/or histopathology from May 2019 to May 2021 were collected retrospectively. 21 kinds of HPV were typed and quantified by BioPerfectus Multiplex Real Time(BMRT)detection kit. The correlation between the viral load of different subtype HPV and the degree of cervical lesions was compared. The optimal cut off value of the viral load of subtype HPV for predicting ≥ HSIL of cervical lesions was analyzed. Results: The top three high infection rates of subtype HPV were HPV 16(45.6%), HPV 18(14.5%), and HPV 52(5.4%). In different cytological grades, the viral load of subtypes HPV16, 18, 58, and 68 had significant differences and were positively correlated with the grades. In different pathological grades, the viral load of subtypes HPV 16, 52, 58 and 68 had significant differences and were positively correlated with the grades of cervical lesions(all P＜0.05). When the cut off values of subtypes HPV16, 52, and 58 reached 4.18, 3.478, and 3.875, the area under the curve(AUC)of subtypes HPV16, 52, and 58 for predicting the risk of cervical lesions ≥ HSIL were 0.839, 0.738, and 0.837, respectively. Conclusion: The viral loads of subtypes HPV are significantly related to the severity of cervical lesions. The detections of HPV by BMRT have certain significance in the primary screening of cervical lesion.
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