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| Correlation between the levels of miR 26a and miR 200a in cord blood of neonates and their respiratory distress syndrome occurrence and adverse outcomes |
| Changxing Branch of Children's Hospital (Changxing County maternal and Child Health Care Hospital), Affiliated to School of Medicine Zhejiang University, Zhejiang Province, 313100 |
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Abstract To investigate the correlation between the levels of miR 26a and miR 200a in cord blood of neonates and the occurrence of their respiratory distress syndrome (NRDS) and their adverse outcomes. Methods: A total of 48 neonates with NRDS were included in study group, and 110 healthy neonates were included in control group from June 2020 to June 2022. The expression levels of miR 26a and miR 200a in cord blood of the neonates were determined by fluorescent quantitative PCR. Pearson method was used to analyze the correlation between the miR 26a and miR 200a expression levels in cord blood of the neonates and their APACHEII score and inflammatory factors. The predictive efficacy of the levels of miR 26a and miR 200a in cord blood for the adverse outcomes of the neonates with NRDS was evaluated by receiver operating characteristic (ROC) curve. Results：The expression levels of miR 26a (0.77±0.20) and miR 200a (0.55±0.15) of the neonates in the study group were significantly lower than those (1.01±0.32 and 1.00±0.29) of the neonates in the control group, and wich of the neonates with severe NRDS were significantly lower than those of the neonates with mild NRDS. The APACHEII score, the levels of inflammatory factors, and the incidences of birth asphyxia and intrauterine distress of the dead neonates all had increased significantly, while the expressions levels of miR 26a and miR 200a of the dead neonates had decreased significantly (all P＜0.05). The mir-26a level of the neonates with NRDS was positively correlated with their expression level of Mir-200A, and was negatively correlated with their APACHEII score and their inflammatory cytokines levels. The mir -200a level of the neonates with NRDS was negatively correlated with heir APACHEII scores and their inflammatory cytokines levels (all P＜0.05). ROC analysis showed that the areas under the curve of mir-26A level and mir-200A level in cord blood, and the combined levels of mir-26A and mir-200A of the neonates with NRDS for predicting their adverse outcomes were 0.774, 0.734, and 0.832, respectively, and the efficiency of the combined levels of mir-26A and mir-200A of the neonates with NRDS for predicting their adverse outcomes was significantly higher (P＜0.05). Conclusion: The expressions of miR 26a and miR 200a in cord blood of the neonates with NRDS decrease, and which is correlation each other. The decreased expression of miR 26a and miR 200a in cord blood of the neonates with NRDS can increase their adverse prognosis, and both of which can be used as the predictors for the adverse prognosis of the neonates.
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