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Abstract To explore the change difference of immune cell profile in decidua of pregnant women with missed abortion or induced abortion. Methods: From January 2019 to December 2021, 51 pregnant women with missed abortion were selected in study group, and another 51 pregnant women with normal early pregnancy who required induced abortion were selected in control group. The decidual tissues of the women in the two groups were collected during abortion. The expressions of immune cells in decidual tissues of the women in the two groups were detected by flow cytometry, and the comparative analysis of which was performed between the two groups. Results: The NK cells (CD56+), macrophages (CD14+), and T lymphocytes (CD3+) dominated the immune cell composition in decidua tissues of the women in the two groups. There were no significant differences in the proportions of CD56+, CD14+, CD3+, CD4+, CD8+, and CD19+ in decidua tissues of the women between the two groups (P＞0.05). In the different subgroups, the proportion of CD49a+CD103+ cells in NK cells, the proportion of M2 cells in macrophages, the proportion of Treg cells in CD4+T cells, and the expression of CD39 in the decidua tissue of the women in the study group were significantly lower than those of the women in the control group. The proportion of M1 cells and the expression of CD80 on the surface of the macrophages, and the expressions of PD-1 and CD161 on the surface of CD4+T and CD8+T cells of the women in the study group were significantly higher than those of the women in the control group (all P＜0.05). Conclusion: Compared with those of the women with normal pregnancy who experienced induced abortion during early pregnancy, the expressions of CD49a+ CD103+ in NK cells, the M1 macrophages, and the T cells with immune killing ability effector in decidual tissue of the pregnant women with missed abortion increase, and their Treg cells with immunosuppressive function decrease, and the changes of which may contribute to the occurrence of missed abortion of the women.
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