To observe the effect of laparoscopic total hysterectomy with uterus free lifting for treating patients with high-grade cervical intraepithelial neoplasia (CIN). Methods: A total of 58 patients with high-grade CIN were selected and were divided into two groups according to the blind drawing method from October 2021 to May 2022. 30 patients in observation group were given laparoscopic total hysterectomy with uterus free lifting, and 28 patients in control group were given of laparoscopic total hysterectomy with uterus lifting. The surgery related indicators of the patients were compared between the two groups. Results: The postoperative pain score (2.0, 1.0-2.25)of the patients in the observation group was significantly lower than that (3.0, 2.0-3.75)of the patients in the control group (P＜0.05). There were no significant differences in the operation time, the intraoperative and postoperative blood loss, the hemoglobin (Hb)value, and the duration of hospital stay of the patients between the two groups (P>0.05). Analysis of the learning curve showed that the operation time (105.0±31.74 min)of the first 15 patients was significantly longer than that (79.67±22.64 min)of the later 15 patients (P＜0.05). Conclusion: Laparoscopic total hysterectomy with uterus free lifting for treating the patients with high-grade CIN is safe and feasible, but which can be mastered by the surgeon after completing the surgeries at least 15 patients.