|
|
|
| Value of MiR-21 level of patients with premature ovarian insufficiency for evaluating the pregnancy outcomes after treated by estradiol combined with dydrogesterone |
| 1. Puyang Maternity and Child Health Care Hospital, Puyang, Henan Province, 457000；2. The Fifth Affiliated Hospital of Zhengzhou University, Henan Province |
|
|
|
|
Abstract To investigate the value of serum miR-21 level of patients with premature ovarian insufficiency (POI) for evaluating the efficacy and their pregnancy outcomes after treated by estradiol combined with dydrogesterone. Methods: 92 patients with POI who had reproductive demand were selected in this study from July 2018 to July 2021. These patients were treated with estradiol combined with dydrogesterone, and the levels of serum miR-21 level of the patients before and after treatment were measured. The correlation between the serum miR-21 level of the patients and their estrogen level was analyzed. Receiver operating characteristic (ROC) curve of the serum miR-21 expression level for diagnosing POI was drawn. The patients were divided into group A (55 patients with high miR-21 expression level ≥ 0.76) and group B (patients with low miR-21 expression level) according to the cutoff value of the serum miR-21 expression level. The pregnancy outcomes of the patients with different miR-21 expression level were analyzed. Results: After treatment, the serum miR-21 and estradiol (E2) levels of the patients with POI had increased significantly, while their follicle stimulating hormone (FSH) and luteinizing hormone (LH) levels had decreased significantly (all P＜0.05). Pearson correlation analysis showed that the serum miR-21 expression level of the patients with POI was positively correlated with their E2 level (r=0.615, P＜0.05), and was negatively correlated with their FSH and LH levels (r=-0.549, -0.511, P＜0.05). After 3 months of treatment, the serum miR-21 expression level of the patients with effective treatment was significantly higher than that of the patients with ineffective treatment (P＜0.05). The AUC of the serum miR-21 level for diagnosing POI of the patients was 0.827, and the optimal cut-off value of which was 0.76. The rates of clinical pregnancy (54.6%) and biochemical pregnancy (65.5%) of the patients in group A were significantly higher than those (18.9% and 29.7%) of the patients in group B (P＜0.05). Conclusion：The serum miR-21 level of the patients with POI has certain value for evaluating the effect treated by estradiol combined with dydrogesterone, and the low expression of serum miR-21 level suggests the increased risk of adverse pregnancy outcomes of the patients.
|
|
|
|
|
|
|
|
|
