To study the value and clinical significance of follicular output rate (FORT) combined with serum let-7f levels for evaluating ovarian response to controlled ovarian hyperstimulation (COH). Methods: The clinical data of 97 women who underwent in vitro fertilization-embryo transfer (IVF-ET) due to the factors of fallopian tube or male from January 2020 to December 2021 were collected. These women were divided into group A (women with low ovarian response), group B (women with medium ovarian response), and group C (women with high ovarian response) according to the number of oocytes retrieved. The FORT, and the serum let-7g and let-7f levels of the women were compared among the three groups. The clinical characteristics, the effect of ovulation induction, the laboratory indicators, and the treatment outcomes of the women with different pregnancy outcomes and FORT were analyzed. The value of FORT, and the levels of serum let-7g and let-7f for evaluating the ovarian response of these women was analyzed. Results: The levels of serum let-7g and let-7f of the women in group B were significantly higher than those of the women in groups A and C (P＜0.05), but which of the women had no significant difference between group A and group C (P＞0.05). The FORT of the women in group A, B, and C had increased gradually (P＜0.05). The serum let-7g and let-7f level, the FORT, the number of eggs obtained, the fertilization rate, and the good quality embryo rate of the women with pregnancy were significantly higher than those of the women without pregnancy (P＜0.05). The AFC number of the women with low FROT was significantly lower than that of the women with medium FROT or with high FROT (P＜0.05), and the number of eggs obtained, the fertilization rate, the good embryo rate, and the clinical pregnancy rate of the women with low FROT, middle FROT, and high FROT had increased gradually (P＜0.05). The serum let-7g level of the women were positively correlated with their et-7f level, but the serum let-7g and let-7f levels of the women were not significantly correlated with their TORF. FORT, the serum let-7g level, and let-7f level for predicting ovarian hyporeactivity or hyperreactivity, the let-7f level had the highest value (AUC=0.754 or 0.767), then followed by FORT (AUC=0.750 or 0.734). The combined of FORT, the serum let-7g level, and the serum let-7f level for predicting ovarian hyporeactivity or hyperreactivity improved the efficiency (AUC=0.859 or 0.848). Conclusion: Both FORT and the serum let-7g and let-7f expression levels have certain efficacy in predicting ovarian response, and which can be used as the potential indicators to guide the formulation of COH regimen.