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| Correlation between the levels of serum anti Jo-1 antibody and anti-double stranded DNA antibody of women and their recurrent abortion |
| Changxing Branch of Children's Hospital, Zhejiang University School of Medicine (Changxing County Maternal and Child Health Care Hospital), Changxing, Zhejiang Province, 313100 |
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Abstract To detect the expressions of anti-double stranded DNA (dsDNA) antibody and anti Jo-1 antibody of women with recurrent abortion, and to explore the correlation between the expression levels of serum anti Jo-1 antibody and anti dsDNA antibody of these women and their recurrent abortion. Methods: A total of 100 women with recurrent abortion were selected in study group, and 100 women without adverse pregnancy history and with normal childbirth history were selected in control group from January 2019 to February 2020. The expression levels of anti Jo-1 antibody, anti dsDNA antibody, and anticardiolipin antibody (ACA) of the women in the two groups were detected by enzyme-linked immunosorbent assay (ELISA). The diagnostic value of anti Jo-1 antibody， anti dsDNA antibody， and ACA of the women for their recurrent abortion was analyzed. Logistic regression analysis was used to analyze the related factors affecting the recurrent abortion of the women. Results: The expression rates of anti-JO-1 antibody (23.0%), anti DSDNA antibody (17.0%), and ACA positive (22.0%) of the women in the study group were significantly higher than those (5.0%, 4.0%, and 8.0%) of the women in the control group (all P＜0.05). In the study group, the positive rate of anti-JO-1 antibody combined with anti-DSDNA antibody detections was significantly higher than that of the anti-JO-1 antibody detection, antiDSDNA antibody detection, or ACA detection alone, or the anti-JO-1 antibody combined with ACA antibody detections, or anti-DSDNA antibody combined with ACA antibody detections (P＜0.05). Receiver operating characteristic (ROC) curve analysis showed that the areas under curves of the anti-JO-1 antibody level, the anti-DSDNA antibody level, and the combination of anti-JO-1 antibody level and anti-DSDNA antibody level of the women for diagnosing their recurrent abortion were 0.590, 0.570, and 0.660, respectively, the sensitivity of which were 95.0%, 97.0%, and 94.0%, respectively, and the specificity of which were 23.0%, 17.0%, and 38.0%, respectively. The serum anti-JO-1 antibody positive expression, anti-DSDNA antibody positive expression, and ACA positive expression of the women were the independent risk factors affecting their recurrent abortion. Conclusion: The anti Jo-1 antibody and anti dsDNA antibody of the women with recurrent abortion are highly expressed, and both of which are the risk factors of recurrent abortion. It is suggested that anti Jo-1 antibody and anti dsDNA antibody can be used as potential biological indicators for early diagnosis of recurrent abortion.
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