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| Correlation between the human chorionic gonadotropin and soluble human leukocyte differentiation antigen-C levels in embryo culture medium of women and their clinical pregnancy after assisted reproduction |
| 1. Meishan City People’s Hospital，Meishan, Sichuan Province, 620010； 2. Shanghai First Maternity and Infant Hospital，Shanghai |
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Abstract To investigate the correlation between the levels of human chorionic gonadotropin(HCG) and soluble human leukocyte differentiation antigen-C(sHLA-G) in embryo culture medium of women during in vitro fertilization- embryo transfer(IVF-ET) and their clinical pregnancy after assisted reproduction. Methods: The clinical data of 120 women who were being treated for infertility and underwent IVF-ET from February 2018 to May 2020 were analyzed retrospectively. Two embryos were transferred for each of 120 patients in every IVF-ET cycle. According to the clinical pregnant situation, 120 woen were divided into group A(60 women with successful pregnancy) and group B(60 women with pregnancy failure). Logistic regression model was used to analyze the risk factors affecting pregnancy of the women after IVF-ET. Spearman method was used to analyze the correlation between the HLA-G and HCG levels of the women and their pregnancy and the number of excellent embryos. Receiver operating characteristic curve(ROC) curve was used to analyze the efficacy of the levels of HLA-G and HCG of the women for predicting their pregnancy outcomes. Results: There were no significant differences in age, insemination method, infertility time, basal sex hormone level, Gn days, Gn total dosage, endometrial thickness, number of oocytes obtained, number of fertilization eggs, and number of embryos of the women between the two groups(P>0.05). The levels of HCG and SHLA-G in embryo culture medium and number of good embryos of the women in group A were significantly higher than those of the women in group B(all P<0.05). Multivariate logistic regression analysis showed that the SHLA-G and HCG levels, and the number of good embryos of the women after IVF-ET were the independent risk factors of their pregnancy(P<0.05). Spearman correlation analysis showed that the SHLA-G and HCG levels of the women were positively correlated with their pregnancy after IVF-ET(r=0.559, 0.682, all P<0.05) and their number of good embryos(r=0.602, 0.564, all P<0.05). ROC curve analysis showed that the area under the curve of HCG level and SHLA-G level for predicting clinical pregnancy were 0.823 and 0.894, respectively. The optimal cut-off value, the sensitivity, the specificity, and Youden index of HCG level for predicting clinical pregnancy were 1.29IU/L, 66.7%, 85.0%, and 0.517, respectively. The optimal cut-off value, the sensitivity, the specificity, and Youden index of SHLA-G level for predicting clinical pregnancy were 4.73 IU/ml, 78.3%, 90.0%, and 0.683, respectively. Conclusion: The levels of HCG and sHLA-G in embryo culture medium of the women after assisted reproduction have certain correlation with their early embryo development and clinical pregnancy, and which can be used as the important reference indicators for predicting pregnancy of the women after assisted reproduction.
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