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Abstract To explore the influence of age and semen pH value of adult males on their sperm quality indicators. Methods：A total of 1246 adult males (excluding azoospermia）who came to hospital for semen routine detections from 2020 to 2021 were selected as the research objects. According to the age, these males were divided into three groups, which included the males with aged ≤30 years in group A, the males with aged 31-35 years in group B, and the males with aged≥36 years in group C. Sperm motility and kinematic parameters were selected as the indicators of sperm quality, and which of the males with different age and with different semen pH were observed. Results：The values of progressive sperm (PR), total sperm motility (PR + non-progressive sperm (NP)), velocity of average path (VAP), velocity of straight-line (VSL), velocity of curve line (VCL), and straight-line sperm motility of the males had significantly difference among group A, B, and C (P<0.01), and which of the males had decreased with their age increase. There was a significant negative correlation between the semen pH value of the males and their NP value (r=-0.064, P<0.05). The values of PR+NP, PR, and VCL of the males with the semen pH value of 7.2 were the highest. The values of VAP and VSL of the males with the semen pH value of 8.0 were the highest, but the NP value of the males with the semen pH value of 8.0 was the lowest. Conclusion：The sperm motility and kinematic parameters of the adult males decrease with their age increase, and which are the best when their semen pH values of the males are 7.2-8.0.
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