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| The expression and significance of murine double minute 4 and microRNA-144-3p in endometrial tissues of infertility patients with endometriosis infertility during the nidation window period |
| Sanmenxia Central Hospital, Henan Province, 472000 |
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Abstract To explore the expression and clinical significance of murine double minute 4 (MDM4) and microRNA-144-3p (miR-144-3p) in endometrial tissues of infertility patients with endometriosis (EMS) during the nidation window period. Methods: During October 2019 to August 2021, 62 infertility patients with EMS who had completed data were included in group A, and 59 infertility patients due to fallopian tube factors were included in group B during the same period. Realtime fluorescence quantitative-polymerase chain reaction (RT-qPCR) method was used to detect the expression levels of MDM4 mRNA and miR-144-3p in the endometrial tissues of the patients in the two groups, and immunohistochemistry was used to detect the expression level of MDM4 protein in endometrial tissue of the patients in the two groups. Pearson method and Spearman method were used to analyze the correlation between the levels of MDM4 mRNA, protein of the patients with EMS in the endometrial tissues during the nidation window period and follicular phase and their miR-144-3p level. Results: The mRNA level and the protein positive rate of MDM4 in the endometrial tissue of the patients in group A were significantly higher than those of the patients in group B, and the mir-144-3p level of the patients in group A was significantly lower than that of the patients in group B (P<0.05). There were no significant differences in the Mir-144-3p and MDM4 mRNA levels, and the positive rate of MDM4 protein of the patients between the two groups (P>0.05). Pearson method analysis showed that the MDM4 mRNA level in the endometrial tissue of the patients with EMS during the nidation window period and follicular phase was negatively correlated with their Mir-144-3p level (r=-0.679, -0.359, P<0.05). Spearman analysis showed that the MDM4 protein level in the endometrial tissue of the patients with EMS during the nidation window period and follicular phase was negatively correlated with their Mir-144-3p levels (r=-0.502, -0.432, P<0.05). Conclusion: The expression of miR-144-3p in the endometrial tissue of the patients with EMS during the nidation window period and follicular phase is low, but the expression of MDM4 is high, and they are negatively correlated each other. Both MiR-144-3p and MDM4 may be jointly involved in the occurrence of infertility caused by EMS.
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