To explore the effect of butorphanol tartrate combined with sufentanil used during perioperative period of patients who had undergone cervical cancer operation. Methods: A total of 360 patients with cervical cancer were selected and randomly divided into observation group and control group (80 cases in each group) from May 2016 to December 2019. The women in the observation group were given butorphanol tartrate combined with sufentanil, and the women in the control group were given sufentanil. The values of perioperative hemodynamic indexes, the visual analog pain score (VAS) at different time point, and Rasmay scores after operation, the rate of adverse reactions, the frequency of postopertative patient controlled intravenous analgesia (PCIA) pressed, and the analgesic satisfaction rate of the patients were compared between the two groups. Results: There were no significant differences in the values of heart rate, systolic blood pressure, and diastolic blood pressure of the patients before entering the operating room, during anesthesia, 30 min after anesthesia, and after the operation between the two groups (P＞0.05). VAS scores of the patients in the observation group at postoperative 6h (2.34±0.71 points), at postoperative 12h (2.25±0.63 points), and at postoperative 24h (2.16±0.57 points) of the patients were significantly lower than those (2.86±0.71 points, 2.71±0.75 points, and 2.58±0.68 points) of the patients in the control group. Rasmay scores of the patients in the observation group at postoperative 6h (3.37±0.71 points), at postoperative 12h (3.48±0.75 points), and at postoperative 24h (3.65±0.76 points) of the patients were significantly higher than those (3.05±0.67 points, 3.11±0.70 points, and 3.26±0.81 points) of the patients in the control group (all P＜0.05). The total incidence (6.7%) of the adverse reactions, such as nausea and vomiting, dizziness, and drowsiness of the patients in the observation group was significantly lower than that (17.2%) of the patients in the control group, the number of PCIA pressed after operation (10.3±3.3 times) of the patients in the observation group was significantly less than that (13.9±4.1 times) of the patients in the control group, and the analgesia satisfaction rate (95.6%) of the patients in the observation group was significantly higher than that (86.1%) of the patients in the control group (all P＜0.05). Conclusion: Butorphanol tartrate combined with sufentanil has good perioperative analgesia effects for the patients with cervical cancer, which has no significantly influence on the intraoperative hemodynamics of the patients, and can reduce the occurrence of adverse reactions and can increase the analgesia satisfaction of the patients.