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| Relatinship between the expression of MicroRNA -26b of patients with endometriosis and their level of cyclooxygenase-2 and the significances of the expression of MicroRNA -26b and the level of cyclooxygenase-2 |
| Rugao People's Hospital, Nantong, Jiangsu Province, 226500 |
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Abstract To observe the expression of cyclooxygenase (COX)-2 in endometrial tissue of patients with endometriosis (EMS). Methods: 195 patients with EMS were selected in study group, and 50 patients without EMS underwent myomectomy or hysterectomy due to cervical lesions were selected in control group from May 2015 to December 2019. The expression of COX-2 protein of these patients was detected by immunohistochemistry and MiR-26b expression in endometrial tissue of these patients was detected by real-time PCR, and the difference of which of the patients were compared between the two groups. The relationship between the COX-2 level of the patients in the study group and their mir-26b expression was analyzed. The relationship between the COX-2 level and the expression of the mir-26b of the patients in the study group and their EMS stage and pain grade was also analyzed. Results: The expression level of COX-2 of the patients in the study group was significantly higher than that of the patients in the control group, but MiR-26b mRNA expression (0.81±0.27) of the patients in the study group was significantly lower than that (1.93±0.46) of the patients in the control group (P＜0.05). There was a positive correlation between the COX-2 expression of the patients in the study group and their pain grade (r=0.724), and there was a weak negative correlation between the Mir-26b expression and their pain grade (r=-0.308) (all P＜0.05). There was no significant correlation between r-AFS stage of Ems of the patients and their expressions of COX-2 and Mir-26b. The expression of COX-2 of the patients in the study group was negatively correlated with their Mir-26b expression (r=-0.522) (P＜0.05). There was no correlation between the COX-2 expression of the patients in the control group and their Mir-26b expression. Conclusion: The expression level of COX-2 in endometrial tissue of the patients with Ems is high, but the expression of MiR-26b is low, and the expression level of COX-2 is negative correlation with the expression of MiR-26b. MiR-26b may be involved in the regulation of COX-2 level in the pathological process of the patients with EMS.
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