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| The expression of NOB1 and miR-21 in cervical lesions tissues of women and its association with the cervical high-risk human papillomavirus infection of the women |
| The Fifth Central Hospital of Tianjin, Tianjin, 300450 |
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Abstract To explore the expressions of NOB1 and miR-21 in cervical lesions tissues of women, and to study its association with the cervical high-risk human papillomavirus (HR-HPV) infection of the women. Methods: The clinical data of 138 women with cervical lesions from January 2018 to May 2020 were selected for retrospective analysis. These women included 52 cases with cervical cancer in group A, 54 cases with cervical squamous intraepithelial lesions in group B, and 32 cases with chronic cervicitis group C. The positive rate of NOB1, the proportion of positive cells, and the comprehensive score of staining intensity of the cells, the expression level of miR-21, the positive rate of HR-HPV, and the DNA load of the women were compared among the three groups. The correlation between the positive rate of NOB1 and the miR-21 level of the women with their HR-HPV infection was analyzed. The efficiency of the positive rate of NOB1 and the miR-21 level of the women for diagnosing the cervical cancer and for predicting their HR-HPV infection were evaluated. Results: The positive rate of NOB1, the proportion of positive cells, the comprehensive score of staining intensity of the cells, and the expression level of Mir-21 of the women in group A, group B, and group C had decreased gradually. The HR-HPV positive rate, and the proportions of HR-HPV DNA load of 100-1000 and ≥1000 of the women in group A were significantly higher than those of the women in the other two groups (P＜0.05), but there was no significant difference in the proportion of HR-HPV DNA load of 1-100 of the women among the three groups (P>0.05). Kendall's TaU-B correlation analysis showed that the proportion of NOB1 positive cells, the comprehensive score of staining intensity of the cells, and the miR21 expression level of the women with HR-HPV infection were positively correlated with their HR-HPV DNA load (r=0.137, 0.143, all P<0.05). Multiple stepwise regression analysis showed that the proportion of NOB1 positive cells and the comprehensive score of staining intensity of the cells were the influencing factors of HR-HPV positive rate. ROC curve analysis showed that the AUC of the proportion of NOB1 positive cells and the comprehensive score of staining intensity of the cells, and the miR21 expression level of the women for diagnosing their cervical cancer were 0.950 and 0.975 (P<0.05). The AUC of the proportion of NOB1 positive cells and the comprehensive score of staining intensity of the cells, and the miR21 expression level of the women for predicting their HR-HPV infection were 0.739 and 0.712 (P<0.05). Conclusion: NOB1 of the women with cervical cancer is positive. The miR-21 level of the women with cervical cancer is high and is positively correlated with their HR-HPV DNA load, which has some values for diagnosing their cervical cancer and for predicting their HR-HPV infection.
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