|
|
|
| Expressions and correlation of anti-Mullerian hormone type II receptor, granulosa cell follicle stimulating hormone receptor, and luteinizing hormone receptor of women with different ovarian reserve |
| 1.Haikou Maternal and Child Health Care Hospital，Haikou, Hainan Province, 571100；2.Hainan Women and Children's Medical Center |
|
|
|
|
Abstract To investigate the expressions and correlation of anti-Mullerian hormone type II receptor(AMHRII), granulosa cell follicle stimulating hormone receptor(FSHR) and luteinizing hormone receptor(LHR) in follicular fluid and granulosa cells of women with different ovarian reserve. Methods: A total of 110 women undergone in vitro fertilization-embryo transfer were enrolled in this study from December 30, 2016 to May 30, 2019. These women were divided into group A(43 cases with normal ovarian function), group B(37 cases with ovarian dysfunction(DOS)), and group C(30 cases with ovarian premature aging(POF)) according to their ovarian function. The follicular fluid and granulosa cells of the women in the three groups were obtained, and the relative expressions of AMHRII, FSHR, and LHR mRNA in follicular fluid and granulosa cells were detected by qRT-PCR. Results: The age, the body mass index value, the FSH and LH levels of the women in group C were significantly higher than those of the women in group A and group B, but the number of ovarian follicles, the number of eggs obtained, and the levels of AMH, estradiol(E2), and progesterone(P) of the women in group C were significantly lower than those of the women in group A and group C(P<0.05). The relative expression levels of AMHR Ⅱ, FSHR, and LHR of the women in group C were the lowest, followed by those of the women in group B, and which of the women in group A were the highest(P<0.05). Correlation analysis showed that FSHR level of the women in group A was positively correlated with their LHR and AMHR Ⅱ expression levels, the LHR level of the women in group A was positively correlated with their AMHR Ⅱ expression level(all P<0.05). In group B, the FSHR level of the women was positively correlated with their LHR and AMHR Ⅱlevels(P<0.05). In group C, the FSHR level of the women was positively correlated with their AMHR Ⅱlevel(P<0.05). Conclusion: The expression levels of AMHRII, FSHR, and LHR of the women with different ovarian reserve are different, which may be related to the mechanism of abnormal ovarian reserve.
|
|
|
|
|
|
|
|
|
