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Abstract To investigate the correlation between the expressions of miR-484 and microtubule-associated protein 2(MAP2)in ovarian malignant cyst tissue of patients and their prognosis. Methods: A total of 154 patients with ovarian malignant cysts from June 2015 to June 2018 were collected in study group retrospectively, and 154 patients with the similar general data who had undergone surgery for benign ovarian cysts were selected in control group. The cyst tissues of the patients in the two groups were collected. The expression of MAP2 in cyst tissues was detected by immunohistochemistry and Western blot, and the expression of miR484 in cyst tissues was detected by real-time fluorescence quantitative PCR(qRT-PCR)method. The correlation between the miR-484 expression and the MAP2 expression was analyzed by Pearson method. Kaplan-Meier method was used to analyze the relationship between the expression levels of different miR-484 and MAP2 of the patients and their survival time. Multivariate Cox regression risk model was used to analyze the prognostic factors of the patients with ovarian malignant cysts. Results: The expression of MAP2 of the patients in the study group was significantly higher than that of the patients in the control group, and the expression of miR-484 was of the patients in the study group was significantly lower, and the expression of MAP2 of the patients in the two groups was negative correlation with their expression of miR-484(r=-0.498)(all P＜0.05). The expressions of MAP2 and miR-484 in ovarian malignant cysts tissues of the patients were not related to their age, pathological type, tumor diameter and degree of invasion, but were related to their degree of differentiation, lymph node metastasis, omental metastasis, FIGO stage, and histopathological grade(all P＜0.05). Kaplan-Meier analysis showed that the 3-year cumulative survival rate(44.9%)of the patients with high MAP2 expression was significantly lower than that(65.8%)of the patients with low MAP2 expression(Log rank χ2=7.432, P=0.006). The 3-year cumulative survival rate(65.8%)of the patients with high miR-484 expression was significantly higher than that(44.0%)of the patients with low miR-484 expression(Log rank χ2=8.819, P=0.003). Cox results showed that the high expression of MAP2, the low expression of miR-484, the lymph node metastasis, the FIGO stage, the degree of differentiation, the high expression of MAP2, the low expression of miR-484, and the omentum metastasis of the patients were all the risk factors affecting their prognosis. Conclusion: The expression of miR-484 in ovarian malignant cyst tissues of the patients is low and the expression of MAP2 is high. The expression of miR-484 and the expression of MAP2 are negatively correlated each other, and both of which are closely related to the development and prognosis of ovarian malignant cysts of the patients.
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