To analyze the influence of laparoscopic hemostasis by suture or bipolar electrocoagulation for treating patients with corpus luteum rupture on their stress hormone level, ovarian function, and immune function. Methods: 127 patients with hemorrhage caused by corpus luteum rupture were included and were divided into group A (66 cases with suture hemostasis) and group B (61 cases with bipolar electrosurgery for hemostasis) according to the different hemostasis methods of laparoscopic surgery from July 2016 to July 2021. The surgical indexes, stress hormones levels, ovarian function, ovarian stromal hemorrhage, immune function indexes levels, and postoperative complications rate of the patients were compared between the two groups. Results: The operation time (41.9±7.5min) and the hospital stay (3.2±0.9d) of the patients in group B were significantly shorter than those (55.3±10.1min and 4.8±1.4d) of the patients in group A. The intraoperative blood loss (50.4±9.6ml) and the postoperative VAS score (3.0±0.9 points) of the patients in group B were significantly lower than those (86.4±12.8ml and 4.6±1.1) of the patients in group A (P＜0.05). The Cor and ADL levels of the patients in both groups after operation had increased significantly, and which of the patients in group B were significantly lower than those of the patients in group A. The levels of FSH, LH, and E2 in both groups after operation had increased significantly, and which of the patients in group B were significantly higher than those of the patients in group A (P＜0.05). The E2 level of the patients in group A after operation had decreased significantly (P＜0.05), but the levels of FSH and LH of the patients in group A after operation had no changes (P＞0.05). The values of AFC and PSV of the patients in group A after operation had no change, but which of the patients in group B after operation had decreased significantly and were significantly lower than those of the patients in group A (P＜0.05). The levels of CD3+, CD4+ and CD8+ of the patients in both groups after operation had decreased significantly, and which of the patients in group B were significantly higher than that of the patients in group A (all P＜0.05). The total complication rate (7.6%) of the patients in group A was significantly lower than that (19.7%) of the patients in group B (P＜0.05). Conclusion: Both suture and bipolar electrocoagulation for hemostasis has their own advantages and disadvantages. Surgical suture hemostasis has little effect on the ovarian stromal hemodynamics and the ovarian function of the patients. Bipolar electrocoagulation hemostasis is more conducive to inhibiting the stress response and protecting the immune function of the patients.