|
|
|
| The expression and significance of extracellular regulatory protein kinase and nuclear factor κB in the endometrium tissue of rats with endometriosis |
| 1.School of Clinical Medicine, Ningxia Medical University, Yinchuan, Ningxia Province, 750004; 2. General Hospital of Ningxia Medical University; 3. Key Laboratory of Fertility Conservation, Ningxia Medical University |
|
|
|
|
Abstract To investigate the expressions of extracellular regulatory protein kinase (ERK1/2) and nuclear factor κB (NF-κBp65) in (EMs) model rat endometrial tissue, and to study their association with the proliferation and invasion of endometriosis cell. Methods: 20 non-pregnant female SD rats were selected, and among them, the autologous transplantation of endometrium was used to induce the establishment of 10 models of rats with Ems and another 10 rats were as control. The expression of ERK1/2 and NF-κBp65 protein in rat endometrial tissue was detected by immunohistochemistry (IHC) and Western blot. The expressions of proliferating cell nuclear antigen (PCNA) and matrix metalloproteinase 9 (MMP9) were used to verify the changes of proliferation and invasiveness of lesions tissue of the ectopic endometrium. Results: In the lesions of model rats with EMs, the enlarged implantation was seen to be transparent or translucent cystic structure, containing light yellow clear or coffee-colored liquid, and blood vessel formation was observed on the surface of cystic structure and around. After hematoxylin eosin stain, the growth of glandular, stroma, or glandular epithelial cells were observed in the ectopic lesion. Immunohistochemical results showed that ERK1/2 was mainly expressed in the cytoplasm of endometrial glandular epithelial cells, and was less expression in the stroma of the cells, and had a very small amount in the nucleus of the cells. NF-κBp65 was mainly expressed in the nuclei of endometrial glands cells and stroma of the cells, and was less expression in the cytoplasm of the cells. Western blot results showed that the expression levels of ERK1/2 and NF-κBp65 protein in ectopic endometrium and eutopic endometrium of the model rats with Ems were significantly higher than those in normal endometrium of the rats, and which in ectopic endometrium of the model rats with Ems were significantly higher than those in eutopic endometrium of the model rats with Ems (P＜0.05). The expression levels of PCNA and MMP9 protein in ectopic endometrium and normal endometrium of the model rats of Ems were significantly higher than those in normal endometrium of the rats, and which in ectopic endometrium of the model rats with Ems were significantly higher than those in eutopic endometrium of the model rats with Ems (P＜0.05). Pearson correlation analysis showed that there was positive correlation between ERK1/2 level and NF-κBp65 protein expression level (r=0.657, P＜0.05) and in eutopic endometrium of the model rats of Ems (r=0.709, P＜0.05). Conclusion: The expressions of ERK1/2 and NF-κBp65 in the eutopic and ectopic endometrial tissues of the EMs rat model increase, which may improve the occurrence and development of EMs by affecting the proliferation and invasion ability of the ectopic endometrium.
|
|
|
|
|
|
|
|
|
