To analyze the expression of leukocyte antigen class I (HLA-I) and microrna-95-5p (miR-95-5p) of women with gestational hypertension, and to study their correlation with the maternal and infant outcomes of the patients. Methods: 95 women with gestational hypertension were selected in study group and 80 normal pregnant women who underwent physical examination were selected in control group from January 2019 to January 2020. The level of HLA-I of the women was detected by enzyme-linked immunosorbent assay（ELISA), and Western Blot was used to detect the protein level of HLA-E, HLA-F, and HLA-G of the patients. The expression of miR-95-5p in blood of the women was detected by RT-PCR. The correlation between the expressions of HLA-I, HLA-E, HLA-F, HLA-G, and miR-95-5p of the women and their maternal and infant outcomes was analyzed. Results: The expressions levels of HLA-I, HLA-E, HLA-F, and HLA-G of the women in the study group were significantly lower than those of the women in the control group, but the expression level of Mir-95-5P of the women in the study group was significantly higher (P<0.05). The expression levels of HLA-I and miR-95-5p of the women with gestational hypertension were correlated with their family history of hypertension and history of gestational hypertension (P<0.05). The incidence of adverse maternal and infant outcomes of the women in the study group was significantly higher than that of the women in the control group (P<0.05). In the women with gestational hypertension, the expression levels of HLA-I molecule of sHLA- I, HLA-E, HLA-F, and HLA-G of the women with adverse maternal and infant outcomes were significantly lower than those of the women with normal maternal and infant outcomes, but the expression of miR-95-5p of the women with adverse maternal and infant outcomes was significantly higher (P<0.05). The expression levels of sHLA-I, HLA-E, HLA-F, and HLA-G of the women were negatively correlated with their Mir-95-5p expression (P<0.05). Conclusion: HLA-I molecule of the women with gestational hypertension is low expression, but miR-95-5p of the women with gestational hypertension is high expression, both of which are related to the maternal and infant outcomes of the women. According to the expression changes of HLA- I molecules and miR-95-5p of the pregnant women with gestational hypertension, their maternal and infant outcomes can be predicted in clinic.