To explore the clinical efficacy of transumbilical laparoendoscopic single-site combined with auxiliary port used in cystectomy of the patients with giant ovarian cyst. Methods: The clinical data of 84 patients with giant ovarian cyst (diameter ≥10cm) from January 2017 to June 2020 were analyzed retrospectively. According to the surgery, these patients were divided 41 patients experienced transumbilical laparoendoscopic single-site cystectomy with auxiliary port in group A and 43 patients experienced routine porous laparoscopic cystectomy in group B. The surgery-related indicators, the satisfaction of abdominal wall appearance, and the complications rate of the patients were compared between the two groups. The pain visual analog scale (VAS) score was evaluated the pain degree of the patients at 24h and 72h after surgery. The levels of serum estradiol (E2), follicle stimulating hormone (FSH), luteinizing hormone (LH), and anti-Müllerian hormone (AMH) of the patients were measured before surgery, and 1 and 3 month after surgery. Results: The operative time (78.7±16.9min) of the patients in group A was significant longer than that of the patients in group B, and the anal exhaust time (25.7±8.1h) of the patients in group A was significant shorter than that of the patients in group B (both P<0.05). There were no significant differences in intraoperative blood loss, postoperative hospital stay time, and postoperative hemoglobin reduction value of the patients between the two groups (P>0.05). The rate of fluid leakage (2.4%) of the patients in group A was significant lower than that (16.3%) of the patients in group B (P<0.05). The VAS scores of the patients in group A in postoperative 24h and 72h were significant lower than those of the patients in group B, and the satisfaction score of abdominal wall appearance of the patients in postoperative 1 month in group A were significant higher than those of the patients in group B (P<0.05). 1 month after surgery, the levels of E2 and AMH of the patients in group A were significant higher than those of the patients in group B, but the levels of FSH and LH of the patients in group A were significant lower than those of the patients in group B (P<0.05), there were no significant differences in the levels of E2, AMH, FSH, and LH of the patients in 3 months after surgery between the two groups (P>0.05). Conclusion: The transumbilical laparoendoscopic single-site combined with auxiliary port used in cystectomy of the patients with giant ovarian cyst has good instrument manipulation and safety, which can reduce the postoperative pain, shorten the recovery time of gastrointestinal function, and improve the satisfaction of abdominal wall appearance, and has lower risk of cystic fluid leakage.